[Dynamics of cellular immunity indices in patients with ophthalmic herpes treated with a herpetic polyvaccine]. / Dinamika nekotorykh pokazatelei kletochnogo immuniteta u bol'nykh oftal'mogerpesom pri lechenii gerpeticheskoi polivaktsinoi.

Specific vaccine therapy resulted in clinical improvement or stabilization of the pathological process in some patients with herpetic conjunctivitis. The vaccine exerted an immunopotentiating effect on the immune status of the subjects under study the direction of which depended upon the initial status of the parameters examined. In some cases the positive clinical effect of the vaccine therapy was accompanied by a decrease in lymphocyte sensitization to herpes simplex virus and normalization of the lymphocyte count.

[Immune status of subjects inoculated with inactivated influenza vaccines]. / Izuchenie immunnogo statusa liudei, privitykh inaktivirovannymi grippoznymi vaktsinami.

Inactivated whole-virion and split-virion influenza vaccines adsorbed on aluminium hydroxide did not suppress the immune system of the vaccinees. Immunization with these vaccine preparations induced the formation of both cell-mediated and humoral immunity in over 60% of the vaccinees.

[Indices of lymphocyte functional activity in patients with recurrent cutaneous-genital herpes during treatment with a herpes vaccine]. / Pokazateli funktsional'noi aktivnosti limfotsitov bol'nykh retsidiviruiushchim kozhno-genital'nym gerpesom v protsesse lecheniia gerpeticheskoi vaktsinoi.

The functional activity of lymphocytes of patients with severe forms of recurrent cutaneous-genital herpes was studied in the course of treatment with inactivated polyvalent herpes vaccine on the basis of lymphocyte blast-transformation response to polyclonal mitogens and herpes simplex virus antigen. In the group of patients who showed a marked therapeutic effect as a result of the treatment the initially high values of the antigen-dependent lymphocyte blast-transformation test declined and became normal. Thus, in some cases the herpes vaccine exerts an immunomodulating effect normalizing the functions of different lymphocyte subpopulations.

[Experimental study of the immunological reactivity of inbred mice with varying sensitivity to the tick-borne encephalitis virus]. / Eksperimental'noe izuchenie immunologicheskoi reaktivnosti inbrednykh myshei s razlichnoi chuvstvitel'nost'iu k virusu kleshchevogo éntsefalita.

Immunological responsiveness of two lines of inbred mice, BALb/c and CBA, was studied by immunization of the animals with concentrated tick-borne encephalitis vaccine, and the role of cell-mediated immunity in formation of resistance of the two genetic lines of mice was evaluated. Determinations of the minimal immunizing dose of the vaccine revealed differences in parameters of the immunizing effect. Studies of the effect of TBE vaccine on the status of cell-mediated immunity by lymphocyte blastogenesis test led to a conclusion that vaccination of BALb/c mice contributed to the realization of the mitogenic effect of the antigen used to much greater extent.

[Radioimmunological study of the antigenic conformity of the hemagglutinins of vaccinal and epidemic strains of influenza virus]. / Izuchenie radioimmunologicheskim metodom antigennogo sootvetstviia gemaggliutininov vaktsinnykh i épidemicheskikh shtammov virusa grippa.

Antigenic relationships of the vaccine A/Victoria/35/72 strain and vaccine candidate A/Khabarovsk/15/76 and recombinant RK-5 strains with hemagglutinins of the epidemic A/Victoria/3/75, A/Leningrad/173/75, and A/Victoria/112/76 strains were studied by radioimmunoassay (RIA) in two test systems. Alongside with other tests RIA is useful and expedient for rapid and accurate quantitative evaluation of the antigenic conformity of influenza virus strains under comparison.

[Heterogeneity of monospecific antibodies to the hemagglutinin of influenza virus H3N2 studied by radioimmunological analysis]. / Izuchenie geterogennosti monospetsificheskikh antitel k gemaggliutininu virusa grippa H3N2 metodom radioimmunologicheskogo analiza.

Heterogeneity of antibodies against influenza virus hemagglutinin has been confirmed. Various subpopulations of antibodies to the A/Khabarovsk/15/76 hemagglutinin were demonstrated some of which showed greater affinity to MRC-11 hemagglutinin than to the HA of the strain inducing this antibody synthesis. Apparently subpopulations of antibodies with low affinity to their "own" epitopes in the hemagglutinin-immunogen may develop which, however, may show a sufficiently high affinity to these epitopes in hemagglutinins of other strains.

Immune response to different tick-borne encephalitis vaccines as revealed by lymphocyte blast transformation and virus neutralization tests.

Immunological memory has been demonstrated in lymphocytes of persons vaccinated against tick-borne encephalitis (TBE). Stimulation indices (SI) of lymphocytes in response to TBE virus antigens in lymphocyte blast transformation test increased after complete vaccination course with the commercial vaccine; by this time, sera of vaccinees contained virus neutralizing antibodies. The concentrated purified vaccine had a higher immunogenic activity, as evidenced by increased SI and virus neutralizing antibodies (NA) in the vaccinated subjects already after the first and second vaccinations.

Blastogenic lymphocyte response as indicator of cell-mediated immunity in humans vaccinated with live and inactivated influenza vaccines.

The level and dynamics of lymphocyte blastogenesis in response to phytohaemagglutinin (PHA) and to specific influenza virus antigen were studied in 3 groups of humans, vaccinated with live or inactivated whole virion influenza vaccines (H3N2 type) and placebo (control group). Both live and inactivated influenza vaccines did not change significantly the functional activity of T lymphocytes as determined by the mean values of stimulation index (SI). The analysis of individual values of PHA-dependent blastogenic response, however, revealed a decrease in SI as compared with its prevaccination level in 33.3 +/- 11.4% of the vaccinees given the live influenza vaccine.

[Blast transformation reaction of the peripheral blood lymphocytes from persons inoculated with a tick-borne encephalitis vaccine]. / Reaktsiia blasttransformatsii limfotsitov perifericheskoi krovi liudei, privitykh vaktsinoi protiv kleshchevogo éntsefalitita.

The indices of blast transformation of lymphocytes to polyclonal mitogens of phytohemagglutinin (PHA) and Pokeweed mitogen (PWM) and to tick-borne encephalitis virus antigen (TBE) were studied in 80 individuals in the course of vaccination against tick-borne encephalitis. In the time course of vaccination reactions of blast transformation of lymphocytes was found to decrease to PHA and to increase to TBE and PWM antigens; spontaneous blast transformation also increased. Increased blast transformation reaction to TBE and PWM antigen correlated with the results of serological studies.

[Antigenic specificity of influenza type A virus hemagglutinin by the method of radioimmunological quantitative analysis]. / Issledovanie antigennoi spetsifichnosti gemaggliutinina virusov grippa tipa A metodom radioimmunologicheskogo kolichestvennogo analiza.

The use of a test system o fa highly purified, antigenically homogeneus hemagglutinin of influenza A/MRC-11 virus and monospecific antiserum permitted to evaluate the potentials of competitive radioimmunoassay in model experiments investigating the antigenic characteristics and content of influenza A (H3N2) virus hemagglutinin. The method proved to be sufficiently specific to differentiate influenza virus strains by antigenic determinants of hemagglutinin, as well as highly sensitive, detecting very small (down to 1 ng) amounts of H3 hemagglutinin in a sample.

[Study of the antigenic specificity of hemagglutinin of influenza viruses type A by quantitative radioimmunoassay. Comparative study of differences in the specificity of H3 hemagglutinin in epidemiologically active strains]. / Issledovanie antigennoi spetsifichnosti gemaggliutinina virusov grippa tipa A metodom radioimmunologicheskogo kolichestvennogo analiza. Sravnitel'noe izuchenie razlichii v spetsifichnosti gemaggliutinina H3 u epidemiologicheski aktivnykh shtammov.

Comparative radioimmunoassays (RIA) of hemagglutinin of influenza type A viruses were carried out. The RIA was shown to detect definite type differences of influenza A and B virus hemagglutinin and subtype differences (HO and H3), as well as to determine both qualitative and quantitative characteristics of renewal of determinants within the antigenic drift. The A/Victoria/3/75 strain was found to have more renewed determinants than the A/Scotland/870/74 strain but less than the A/Victoria/112/76 strain which could be considered as a next drift variant. The epidemic strains circulating after 1972 had no more than 10% of the antigenic determinants characteristics of the original A/Hong Kong/1/68 strain. One of the advantages of the RIA was demonstrated to be its high sensitivity and economy as the specific antiserum can be used in dilutions 1:20,000 and higher. The test antigens may be both freshly isolated strains and virus-containing fluids showing no hemagglutinating activity (a titer in HA test 1:0).