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1.
Antimicrob Agents Chemother ; 53(5): 1874-83, 2009 May.
Article in English | MEDLINE | ID: mdl-19223616

ABSTRACT

Antibiotic treatment of Staphylococcus aureus infections is often problematic due to the slow response to therapy and the high frequency of infection recurrence. The intracellular persistence of staphylococci has been recognized and could offer a good explanation for these treatment difficulties. Knowledge of the interplay between intracellular antibiotic activity and the overall outcome of infection is therefore important. Several intracellular in vitro models have been developed, but few experimental animal models have been published. The mouse peritonitis/sepsis model was used as the basic in vivo model exploring a quantitative ex vivo extra- and intracellular differentiation assay. The intracellular presence of S. aureus was documented by electron microscopy. Five antibiotics, dicloxacillin, cefuroxime, gentamicin, azithromycin, and rifampin (rifampicin), were tested in the new in vivo model; and the model was able to distinguish between their extra- and intracellular effects. The intracellular effects of the five antibiotics could be ranked as follows as the mean change in the log(10) number of CFU/ml (Delta log(10) CFU/ml) between treated and untreated mice after 4 h of treatment: dicloxacillin (3.70 Delta log(10) CFU/ml) > cefuroxime (3.56 Delta log(10) CFU/ml) > rifampin (1.86 Delta log(10) CFU/ml) > gentamicin (0.61 Delta log(10) CFU/ml) > azithromycin (0.21 Delta log(10) CFU/ml). We could also show that the important factors during testing of intracellular activity in vivo are the size, number, and frequency of doses; the time of exposure; and the timing between the start of infection and treatment. A poor correlation between the intracellular accumulation of the antibiotics and the actual intracellular effect was found. This stresses the importance of performing experimental studies, like those with the new in vivo model described here, to measure actual intracellular activity instead of making predictions based on cellular pharmacokinetic and MICs.


Subject(s)
Anti-Bacterial Agents/pharmacology , Peritonitis , Sepsis , Staphylococcal Infections , Staphylococcus aureus/drug effects , Animals , Animals, Outbred Strains , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/pharmacokinetics , Anti-Bacterial Agents/therapeutic use , Colony Count, Microbial , Dicloxacillin/administration & dosage , Dicloxacillin/pharmacokinetics , Dicloxacillin/pharmacology , Dicloxacillin/therapeutic use , Dose-Response Relationship, Drug , Female , Humans , Mice , Microbial Sensitivity Tests , Peritoneum/cytology , Peritoneum/drug effects , Peritoneum/microbiology , Peritonitis/drug therapy , Peritonitis/microbiology , Rifampin/administration & dosage , Rifampin/pharmacokinetics , Rifampin/pharmacology , Rifampin/therapeutic use , Sepsis/drug therapy , Sepsis/microbiology , Staphylococcal Infections/drug therapy , Staphylococcal Infections/microbiology , Treatment Outcome
2.
Open Microbiol J ; 2: 29-31, 2008.
Article in English | MEDLINE | ID: mdl-19088908

ABSTRACT

Helicobacter cinaedi is an infrequent, but well recognized cause of gastroenteritis in immunosuppressed patients. Here we report a case of an extra-intestinal infection in a previous healthy 61-year old heterosexual male. Focus for the infection was most likely cellulitis on the lower right leg. The bacterium was cultured from blood twice within one week. Electron microscopy of the isolate visualized bipolar flagella. Partial DNA sequencing of the 16S rRNA gene and phenotypic characterization of the isolate established the species diagnosis. The patient was treated with rifampicin. After end of treatment blood cultures were negative and the cellulitis had disappeared.

3.
FEMS Immunol Med Microbiol ; 50(2): 264-7, 2007 Jul.
Article in English | MEDLINE | ID: mdl-17567285

ABSTRACT

On the basis of biochemical, phenotypic and 16S rRNA analyses, Helicobacter canis was isolated and identified from an otherwise healthy 7-month-old girl with intermittent fever. Blood cultures signalled bacterial growth after 5 days that was characterized as small gram-negative spiral rods. Subculturing on Colombia plates with 5% sheep blood, chocolate agar and brucella agar, aerobically and anaerobically as well as in a microaerophilic atmosphere, showed scanty growth after an additional 4 days. Secondarily seeded with fluid from the original bottle, the paediatric blood bottles repeatedly signalled growth after one night's incubation, whereas the conventially treated bottles did not support growth after 7 days' incubation. From the secondary seeded paediatric bottles a pure culture was isolated on chocolate agar plates, and identified as H. canis. This case indicates that blood culture systems should be compared and improved for their capacity to detect Helicobacter and related pathogenic bacteria species. Further studies are also needed to determine the importance of H. canis as a primary pathogen, and the role of cats in the possible zoonotic spread of H. canis to humans.


Subject(s)
Bacteremia/microbiology , Helicobacter Infections/microbiology , Helicobacter/classification , Bacterial Typing Techniques , Female , Genes, rRNA , Helicobacter/genetics , Helicobacter/isolation & purification , Helicobacter/ultrastructure , Humans , Infant , Microscopy, Electron, Transmission , Molecular Sequence Data , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid
4.
J Clin Microbiol ; 45(4): 1366-9, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17267638

ABSTRACT

A Caulobacter sp. isolate was recovered from the dialysis fluid of a patient undergoing peritoneal dialysis. Bacterial identification included electron microscopy and 16S rDNA sequencing. To our knowledge, this is the first report of human Caulobacter infection. Special growth requirements suggest that Caulobacter spp. may be overlooked in the clinical microbiology laboratory.


Subject(s)
Caulobacter/isolation & purification , Gram-Negative Bacterial Infections/microbiology , Peritonitis/microbiology , Anti-Bacterial Agents/pharmacology , Bacterial Typing Techniques , Caulobacter/genetics , Caulobacter/ultrastructure , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Dialysis Solutions , Humans , Male , Microbial Sensitivity Tests , Microscopy, Electron, Transmission , Middle Aged , Peritoneal Dialysis , Sequence Homology, Nucleic Acid
5.
J Virol Methods ; 134(1-2): 92-8, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16417929

ABSTRACT

It is possible to visualize rapidly viral particles by electron microscopy (EM) in patient samples and in cell cultures, and characterize the particles on the basis of their size and morphology. In many instances, EM has contributed to the diagnosis of specific infectious agents. Four different types of viruses with different characteristics of particle size, capsid structure, the presence or absence of an envelope, genomic content and stability outside the host were screened and diagnosed by EM at the level of family/genus. The results were confirmed at the species level by elution of the sample material from the grids used for EM examination and nucleic acid amplification. This approach could be valuable in situations where the immediate diagnosis is unclear, or when new infectious agents appear.


Subject(s)
Adenoviridae/isolation & purification , Cytomegalovirus/isolation & purification , Influenza A virus/isolation & purification , Microscopy, Electron, Transmission , Norovirus/isolation & purification , Nucleic Acid Amplification Techniques , Adenoviridae/genetics , Adenoviridae/ultrastructure , Adenoviridae Infections/diagnosis , Adenoviridae Infections/virology , Animals , Caliciviridae Infections/diagnosis , Caliciviridae Infections/virology , Cytomegalovirus/genetics , Cytomegalovirus/ultrastructure , Cytomegalovirus Infections/diagnosis , Cytomegalovirus Infections/virology , DNA, Viral/genetics , Eye/virology , Feces/virology , Humans , Influenza A virus/genetics , Influenza A virus/ultrastructure , Influenza, Human/diagnosis , Influenza, Human/virology , Norovirus/genetics , Norovirus/ultrastructure , RNA, Viral/genetics , Sensitivity and Specificity , Species Specificity , Tissue Embedding , Trachea/virology , Urine/virology
6.
Infect Immun ; 73(8): 4626-33, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16040975

ABSTRACT

The capsular polysaccharide and type 1 fimbriae are two of the major surface-located virulence properties associated with the pathogenesis of Klebsiella pneumoniae. The capsule is an elaborate polysaccharide matrix that encases the entire cell surface and provides resistance against many host defense mechanisms. In contrast, type 1 fimbriae are thin adhesive thread-like surface organelles that can extend beyond the capsular matrix and mediate d-mannose-sensitive adhesion to host epithelial cells. These fimbriae are archetypical and consist of a major building block protein (FimA) that comprises the bulk of the organelle and a tip-located adhesin (FimH). It is assumed that the extended major-subunit protein structure permits the FimH adhesin to function independently of the presence of a capsule. In this study, we have employed a defined set of K. pneumoniae capsulated and noncapsulated strains to show that the function of type 1 fimbriae is actually impeded by the concomitant expression of a polysaccharide capsule. Capsule expression had significant effects on two parameters commonly used to define FimH function, namely, yeast cell agglutination and biofilm formation. Our data suggest that this effect is not due to transcriptional/translational changes in fimbrial gene/protein expression but rather the result of direct physical interference. This was further demonstrated by the fact that we could restore fimbrial function by inhibiting capsule synthesis. It remains to be determined whether the expression of these very different surface components occurs simply via random events of phase variation or in a coordinated manner in response to specific environmental cues.


Subject(s)
Bacterial Capsules/metabolism , Fimbriae, Bacterial/metabolism , Klebsiella pneumoniae/metabolism , Bacterial Capsules/genetics , Bacterial Capsules/ultrastructure , Biofilms , Fimbriae Proteins/biosynthesis , Fimbriae Proteins/genetics , Fimbriae, Bacterial/genetics , Fimbriae, Bacterial/ultrastructure , Klebsiella pneumoniae/ultrastructure , Microscopy, Electron , Promoter Regions, Genetic
7.
J Microbiol Methods ; 62(2): 233-43, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16009280

ABSTRACT

An indirect enzyme-linked immunosorbent assay (ELISA) based on Salmonella re-polymerized flagella was employed to measure levels of immunoglobulin (Ig) G, IgM and IgA antibodies in sera from 303 Danish patients diagnosed with either Salmonella enteritidis or Salmonella typhimurium. The antibody-levels were assessed at one, three and six months after onset of salmonellosis, and sera from a control-group of 170 healthy blood donors were additionally analysed in order to establish cut-off values for the analysis. Cross-reactions to other Salmonella serotypes, as well as to Escherichia coli, Yersinia enterocolitica, Campylobacter jejuni, Campylobacter coli and Helicobacter pylori were observed. At one month after onset of symptoms, 70% of the patients recovering from a S. enteritidis infection carried detectable levels of anti-flagella antibodies, as did 77% of the patients recovering from S. typhimurium infection. Three months after onset of symptoms these detection rates had decreased to 46% and 40%; and six months after onset of symptoms the detection rates were 34% and 38%. This rapid decrease in the serum levels of flagella antibodies is in conflict with the "common knowledge" statement of a long-lasting anti-flagella immunoresponse. The present study suggests that such a tenacious statement is (or may be) inaccurate.


Subject(s)
Antibodies, Bacterial/blood , Gastroenteritis/immunology , Gastroenteritis/microbiology , Salmonella Infections/immunology , Salmonella/immunology , Adolescent , Adult , Aged , Aged, 80 and over , Antigens, Bacterial/immunology , Antigens, Bacterial/ultrastructure , Child , Denmark , Enzyme-Linked Immunosorbent Assay , Female , Flagella/immunology , Flagella/microbiology , Flagella/ultrastructure , Follow-Up Studies , Humans , Male , Mass Spectrometry , Microscopy, Electron , Middle Aged , Salmonella Infections/microbiology , Salmonella enteritidis/immunology , Salmonella typhimurium/immunology
8.
Appl Environ Microbiol ; 68(1): 11-9, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11772603

ABSTRACT

Helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called U forms. The importance of nonculturable coccoid forms of H. pylori in disease transmission and antibiotic treatment failures is unclear. Metabolic activities of actively growing as well as nonculturable H. pylori were investigated by comparing the concentrations of cellular ATP and total RNA, gene expression, presence of cytoplasmic polyphosphate granules and iron inclusions, and cellular morphology during extended broth culture and nutritional cold starvation. In addition, the effect of exposing broth-cultured or cold-starved cells to a nutrient-rich or acidic environment on the metabolic activities was investigated. ATP was detectable up to 14 days and for at least 25 days after transformation from the spiral form to the coccoid form or U form in broth-cultured and cold-starved cells, respectively. mRNAs of VacA, a 26-kDa protein, and urease A were detected by using reverse transcription-PCR in cells cultured for 2 months in broth or cold starved for at least 28 months. The ATP concentration was not affected during exposure to fresh or acidified broth, while 4- to 12-h exposures of nonculturable cells to lysed human erythrocytes increased cellular ATP 12- to 150-fold. Incubation of nonculturable cold-starved cells with an erythrocyte lysate increased total RNA expression and ureA mRNA transcription as measured by quantitative real-time reverse transcription-PCR. Furthermore, the number of structurally intact starved coccoids containing polyphosphate granules increased almost fourfold (P = 0.0022) under the same conditions. In conclusion, a specific environmental stimulus can induce ATP, polyphosphate, and RNA metabolism in nonculturable H. pylori, indicating viability of such morphological forms.


Subject(s)
Cold Temperature , Gene Expression Regulation, Bacterial , Heat-Shock Response , Helicobacter pylori/metabolism , Adenosine Triphosphate/metabolism , Culture Media , Helicobacter pylori/growth & development , Helicobacter pylori/ultrastructure , Hydrogen-Ion Concentration , Microscopy, Electron , RNA, Bacterial/metabolism , RNA, Messenger/metabolism
9.
J Med Microbiol ; 50(1): 96-100, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11192512

ABSTRACT

Leptospira fainei serovar Hurstbridge is a recently discovered Leptospira species and so far it has only been cultured from animal sources. Based on positive serology and positive PCR for L. fainei among patients suspected of having leptospirosis, a role in human disease seems likely. This study describes two patients with Weil's disease from whom L. fainei was cultured. A local source of the infections was suspected, as these two patients resided in the same area of Denmark, were hospitalised approximately at the same time and had not been travelling recently. The Leptospira species was determined by serology, PCR and sequencing of bacterial DNA. One patient developed autoimmune hepatitis in the course of the L. fainei infection and was treated with both antibiotics and immunosuppression with good effect. The other patient had a self-limiting disease and did not receive any treatment.


Subject(s)
Leptospira/classification , Leptospira/isolation & purification , Weil Disease/microbiology , Culture Media , DNA, Bacterial/analysis , DNA, Bacterial/genetics , DNA, Ribosomal/analysis , DNA, Ribosomal/genetics , Humans , Leptospira/genetics , Leptospira/immunology , Male , Microscopy, Electron , Middle Aged , Molecular Sequence Data , Polymerase Chain Reaction , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA
10.
J Med Microbiol ; 50(12): 1087-1094, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11761194

ABSTRACT

The pathogenesis of campylobacter infection in man is largely unknown, although cytolethal distending toxin (CDT) has been incriminated as a virulence factor. However, little is known about the cdt genes in Campylobacter spp. isolated from broiler chickens. A total of 350 cloacal swabs was collected and tested by conventional culture and PCR. Of the 114 Campylobacter isolates obtained, 101 (88.6%) were identified as C. jejuni and 13 (11.4%) as C. coli by conventional methods. cdt genes were detected by PCR in all the isolates except one C. jejuni isolate. Cytotoxic effects were produced in a Vero cell line, by 100 of the C. jejuni isolates. In contrast, 10 C. coli isolates produced much lower levels of toxin and 3 produced no detectable toxin. These results confirm the common occurrence of campylobacter infection in chickens and indicate that cdt genes are commonly present in both C. jejuni and C. coli isolates from broilers, but that there are distinct differences in CDT production in these two closely related species.


Subject(s)
Bacterial Toxins/genetics , Campylobacter Infections/veterinary , Campylobacter/genetics , Chickens , Poultry Diseases/microbiology , Animals , Bacterial Toxins/toxicity , Base Sequence , Campylobacter/isolation & purification , Campylobacter Infections/epidemiology , Campylobacter Infections/microbiology , Cells, Cultured , Chick Embryo , Chlorocebus aethiops , DNA, Bacterial/analysis , Denmark/epidemiology , Feces/microbiology , Polymerase Chain Reaction/veterinary , Poultry Diseases/epidemiology , Prevalence , Vero Cells
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