ABSTRACT
Chitinases are ubiquitous chitin-fragmenting hydrolases. Recently we discovered the first human chitinase, named chitotriosidase, that is specifically expressed by phagocytes. We here report the identification, purification, and subsequent cloning of a second mammalian chitinase. This enzyme is characterized by an acidic isoelectric point and therefore named acidic mammalian chitinase (AMCase). In rodents and man the enzyme is relatively abundant in the gastrointestinal tract and is found to a lesser extent in the lung. Like chitotriosidase, AMCase is synthesized as a 50-kDa protein containing a 39-kDa N-terminal catalytic domain, a hinge region, and a C-terminal chitin-binding domain. In contrast to chitotriosidase, the enzyme is extremely acid stable and shows a distinct second pH optimum around pH 2. AMCase is capable of cleaving artificial chitin-like substrates as well as crab shell chitin and chitin as present in the fungal cell wall. Our study has revealed the existence of a chitinolytic enzyme in the gastrointestinal tract and lung that may play a role in digestion and/or defense.
Subject(s)
Chitinases/metabolism , Hexosaminidases/metabolism , Amino Acid Sequence , Animals , Base Sequence , Chitinases/chemistry , Chitinases/genetics , DNA, Complementary/chemistry , Hexosaminidases/chemistry , Hexosaminidases/genetics , Humans , Hydrogen-Ion Concentration , Isoelectric Point , Mice , Molecular Sequence Data , Molecular Weight , RNA, Messenger/analysis , Species SpecificityABSTRACT
Recent research carried out in several laboratories has indicated that, in addition to their role as intermediates in many metabolic pathways, amino acids can interact with insulin-dependent signal transduction. In this short review, the current state of this rapidly expanding field is discussed.
Subject(s)
Amino Acids/metabolism , Signal Transduction , Signal Transduction/physiologyABSTRACT
3-Methyladenine which stops macroautophagy at the sequestration step in mammalian cells also inhibits the phosphoinositide 3-kinase (PI3K) activity raising the possibility that PI3K signaling controls the macroautophagic pathway (Blommaart, E. F. C., Krause, U., Schellens, J. P. M., Vreeling-Sindelárová, H., and Meijer, A. J. (1997) Eur. J. Biochem. 243, 240-246). The aim of this study was to identify PI3Ks involved in the control of macroautophagic sequestration in human colon cancer HT-29 cells. An increase of class I PI3K products (phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-triphosphate) caused by either feeding cells with synthetic lipids (dipalmitoyl phosphatidylinositol 3, 4-bisphosphate and dipalmitoyl phosphatidylinositol 3,4, 5-triphosphate) or by stimulating the enzymatic activity by interleukin-13 reduced macroautophagy. In contrast, an increase in the class III PI3K product (phosphatidylinositol 3-phosphate), either by feeding cells with a synthetic lipid or by overexpressing the p150 adaptor, stimulates macroautophagy. Transfection of a specific class III PI3K antisense oligonucleotide greatly inhibited the rate of macroautophagy. In accordance with a role of class III PI3K, wortmannin (an inhibitor of PI3Ks) inhibits macroautophagic sequestration and protein degradation in the low nanomolar range (IC(50) 5-15 nM). Further in vitro enzymatic assay showed that 3-methyladenine inhibits the class III PI3K activity. Dipalmitoyl phosphatidylinositol 3-phosphate supplementation or p150 overexpression rescued the macroautophagic pathway in HT-29 cells overexpressing a GTPase-deficient mutant of the Galpha(i3) protein suggesting that both class III PI3K and trimeric G(i3) protein signaling are required in the control macroautophagy in HT-29 cells. In conclusion, our results demonstrate that distinct classes of PI3K control the macroautophagic pathway in opposite directions. The roles of PI3Ks in macroautophagy are discussed in the context of membrane recycling.
Subject(s)
Autophagy/physiology , Phosphatidylinositol 3-Kinases/metabolism , Phosphatidylinositol Phosphates/metabolism , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins/metabolism , Signal Transduction/physiology , Adenocarcinoma , Androstadienes/pharmacology , Autophagy/drug effects , Chromones/pharmacology , Colonic Neoplasms , Enzyme Inhibitors/pharmacology , Homeostasis , Humans , Isoenzymes/genetics , Isoenzymes/metabolism , Kinetics , L-Lactate Dehydrogenase/analysis , Morpholines/pharmacology , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositol Phosphates/pharmacology , Proto-Oncogene Proteins c-akt , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured , WortmanninABSTRACT
The rate of proteolysis is an important determinant of the intracellular protein content. Part of the degradation of intracellular proteins occurs in the lysosomes and is mediated by macroautophagy. In liver, macroautophagy is very active and almost completely accounts for starvation-induced proteolysis. Factors inhibiting this process include amino acids, cell swelling and insulin. In the mechanisms controlling macroautophagy, protein phosphorylation plays an important role. Activation of a signal transduction pathway, ultimately leading to phosphorylation of ribosomal protein S6, accompanies inhibition of macroautophagy. Components of this pathway may include a heterotrimeric Gi3-protein, phosphatidylinositol 3-kinase and p70S6 kinase. Recent evidence indicates that lysosomal protein degradation can be selective and occurs via ubiquitin-dependent and -independent pathways.
Subject(s)
Autophagy/physiology , Liver/metabolism , Lysosomes/metabolism , Proteins/metabolism , Amino Acids/metabolism , Animals , Autophagy/drug effects , Cell Size , Cytosol/metabolism , Humans , Insulin/metabolism , Insulin Secretion , Liver/cytology , Liver/ultrastructure , Lysosomes/drug effects , Lysosomes/ultrastructure , Microscopy, Electron , Neoplasms/metabolism , Neoplasms/pathology , Phosphoprotein Phosphatases/metabolism , Phosphorylation , Protein Kinases/metabolism , Ribosomal Proteins/metabolism , Sensitivity and Specificity , Signal Transduction/drug effects , Signal Transduction/physiology , Ubiquitins/pharmacologyABSTRACT
Recent studies indicate that phosphatidylinositol 3-kinase is essential in the regulation of many processes dependent on membrane flow. Autophagy is a complex pathway in which cell material, including proteins, can be degraded. Membrane flow plays a pivotal role in this process. To find out whether phosphatidylinositol 3-kinase is also required for autophagy, we tested the effects on autophagy of two structurally unrelated phosphatidylinositol 3-kinase inhibitors, wortmannin and 2-(4-morpholinyl)-8-phenylchromone (LY294002). The addition of low concentrations of each of these inhibitors to incubations of hepatocytes in the absence of amino acids resulted in a strong inhibition of proteolysis. The antiproteolytic effect of wortmannin (IC50 30 nM) and LY294002 (IC50 10 microM) was accompanied by inhibition of autophagic sequestration and not by an increase in lysosomal pH or a decrease in intracellular ATP. No further inhibition of proteolysis by the two compounds was observed when autophagy was already maximally inhibited by high concentrations of amino acids. 3-Methyladenine, which is commonly used as a specific inhibitor of autophagic sequestration, was an inhibitor of phosphatidylinositol 3-kinase, thus providing a target for its action. It is proposed that phosphatidylinositol 3-kinase activity is required for autophagy. 3-Methyladenine inhibits autophagy by inhibition of this enzyme.
Subject(s)
Androstadienes/pharmacology , Autophagy/drug effects , Chromones/pharmacology , Enzyme Inhibitors/pharmacology , Liver/cytology , Morpholines/pharmacology , Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors , Adenine/analogs & derivatives , Adenine/pharmacology , Animals , Cell Membrane/physiology , Cells, Cultured , Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/metabolism , Male , Phosphatidylinositol 3-Kinases , Phosphorylation , Protein Serine-Threonine Kinases/metabolism , Rats , Rats, Wistar , Ribosomal Protein S6 , Ribosomal Protein S6 Kinases , Ribosomal Proteins/metabolism , Signal Transduction , Starvation/physiopathology , WortmanninSubject(s)
Autophagy , Liver/metabolism , Proteins/metabolism , Amino Acids/metabolism , Amino Acids/pharmacology , Androstadienes/pharmacology , Animals , Cells, Cultured , Chromones/pharmacology , Enzyme Inhibitors/pharmacology , Food Deprivation , Glucagon/pharmacology , Insulin/pharmacology , Male , Morpholines/pharmacology , Phosphates/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Phosphoinositide-3 Kinase Inhibitors , Phosphorylation , Polyenes/pharmacology , Rats , Rats, Wistar , Ribosomal Protein S6 , Ribosomal Protein S6 Kinases/antagonists & inhibitors , Ribosomal Protein S6 Kinases/metabolism , Ribosomal Proteins/metabolism , Sirolimus , Sucrose/metabolism , WortmanninABSTRACT
In rat hepatocytes, autophagy is known to be inhibited by amino acids. Insulin and cell swelling promote inhibition by amino acids. Each of the conditions leading to inhibition of autophagic proteolysis was found to be associated with phosphorylation of a 31-kDa protein that we identified as ribosomal protein S6. A combination of leucine, tyrosine, and phenylalanine, which efficiently inhibits autophagic proteolysis, was particularly effective in stimulating S6 phosphorylation. The relationship between the percentage inhibition of proteolysis and the degree of S6 phosphorylation was linear. Thus, inhibition of autophagy and phosphorylation of S6 are under the control of the same signal transduction pathway. Stimulation of S6 phosphorylation by the presence of amino acids was due to activation of S6 kinase and not to inhibition of S6 phosphatase. The inhibition by amino acids of both autophagic proteolysis and autophagic sequestration of electro-injected cytosolic [14C]sucrose was partially prevented by rapamycin, a compound known to inhibit activation of p70 S6 kinase. In addition, rapamycin partially inhibited the rate of protein synthesis. We conclude that the fluxes through the autophagic and protein synthetic pathways are regulated in an opposite manner by the degree to which S6 is phosphorylated. Possible mechanisms by which S6 phosphorylation can cause inhibition of autophagy are discussed.