Voice Outcomes Following Multiple Surgeries for Recurrent Respiratory Papillomatosis.

OBJECTIVES: Recurrent Respiratory Papillomatosis (RRP) is a rare disease caused by the human papilloma virus that presents as warty, exophytic growths in the upper airway. RRP in the larynx can lead to severe airway obstruction and voice changes. It is clinically known that patients with RRP frequently experience dysphonia. The purpose of this study was to assess the impact of multiple surgical treatments on RRP patients' voice outcomes, and to determine whether a higher number of repeated surgeries lead to decreased voice quality. METHODS: A retrospective cohort study was conducted of adult RRP patients (n = 23) who underwent multiple surgeries. Group 1 included patients (n = 11) who had <5 surgeries (range 2-4 surgeries) and Group 2 included patients (n = 12) who had ≥5 surgeries (range 5-50+ surgeries). Voice recordings were selected from the following clinic visits: initial office visit (initial presurgery), first postsurgery, and the last clinic encounter(s) with no immediate planned surgery. Blinded auditory-perceptual ratings along with cepstral and spectral acoustic measures assessed voice severity. RESULTS: There was significant improvement from the initial presurgery visit compared to each postoperative visit over time for all voice outcome measures for both Group 1 and Group 2. The results of the study indicated that the number of surgeries did not significantly affect mean postoperative voice outcomes, and there were no significant differences between patient groups for voice quality over time. CONCLUSIONS: The results of this study suggest that RRP patients' voice quality may not suffer cumulative negative effects when using modern tissue-sparing surgical techniques.

Effects of Volume, Pitch, and Phonation Type on Oscillation Initiation and Termination Phases Investigated With High-speed Videoendoscopy.

OBJECTIVES: This study aimed to investigate the effects of varying volume, pitch, and phonation types on the initiation and termination phases of vocal fold oscillation using high-speed digital videoendoscopy. Specifically, it addressed the effects of the variation of volume, pitch, and phonation type (normal, pressed, and breathy) on the transient duration of the vibrating glottal length (length transient duration, Tlen), the transient duration of the glottal area waveform (area transient duration, Tarea), the time offset between the beginning (or the end) of the full-length vibration and the full-amplitude vibration, TΔ, and the variation of the fundamental frequency during the vocal fold oscillation initiation and termination segments (pitch instability, %PI). METHODS: A female subject with no voice problem produced voices with varying pitch and loudness, including comfortable pitch and comfortable loudness, normal pitch loud, high pitch and comfortable loudness, and high pitch and loud. Breathy and pressed phonations were also recorded. Each of the six phonation types was recorded six times, which resulted in 72 transient segments (each recording included both initiation and termination phases). Mixed model statistical analyses were employed to the five objective high-speed digital videoendoscopy parameters. RESULTS: Preliminary findings demonstrated significant findings for voice type effects for the length and area transient durations for the oscillation initiation segment but not for the oscillation termination segment. CONCLUSIONS: This study demonstrates that voice types appear to influence vibration initiation patterns more than the vibration termination patterns.

Comparative Effectiveness of Potential Elicitors of Plant Resistance against Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in Four Crop Plants.

Feeding by insect herbivores activates plant signaling pathways, resulting in the enhanced production of secondary metabolites and other resistance-related traits by injured plants. These traits can reduce insect fitness, deter feeding, and attract beneficial insects. Organic and inorganic chemicals applied as a foliar spray, seed treatment, or soil drench can activate these plant responses. Azelaic acid (AA), benzothiadiazole (BTH), gibberellic acid (GA), harpin, and jasmonic acid (JA) are thought to directly mediate plant responses to pathogens and herbivores or to mimic compounds that do. The effects of these potential elicitors on the induction of plant defenses were determined by measuring the weight gains of fall armyworm, Spodoptera frugiperda (J. E. Smith) (FAW) (Lepidoptera: Noctuidae) larvae on four crop plants, cotton, corn, rice, and soybean, treated with the compounds under greenhouse conditions. Treatment with JA consistently reduced growth of FAW reared on treated cotton and soybean. In contrast, FAW fed BTH- and harpin-treated cotton and soybean tissue gained more weight than those fed control leaf tissue, consistent with negative crosstalk between the salicylic acid and JA signaling pathways. No induction or inconsistent induction of resistance was observed in corn and rice. Follow-up experiments showed that the co-application of adjuvants with JA failed to increase the effectiveness of induction by JA and that soybean looper [Chrysodeixis includens (Walker)], a relative specialist on legumes, was less affected by JA-induced responses in soybean than was the polyphagous FAW. Overall, the results of these experiments demonstrate that the effectiveness of elicitors as a management tactic will depend strongly on the identities of the crop, the pest, and the elicitor involved.

Integrating flood depth and plant resistance with chlorantraniliprole seed treatments for management of rice water weevil, Lissorhoptrus oryzophilus (Coleoptera: Curculionidae).

Chlorantraniliprole seed treatments in rice provide effective suppression of rice water weevil populations in the United States; however, heavy reliance on prophylactic insecticide treatments as a sole strategy could destabilize management programs for this insect. The present research evaluated the compatibility of seed treatments with two other potential management tactics-plant resistance and shallow flooding-by conducting two split-plot experiments in 2009 and 2011. In both experiments, no substantial antagonism was found among the 3 different tactics. Statistical interactions in these experiments arose from the strong and persistent effects of chlorantraniliprole on larval densities rather than incompatibility of tactics. In 2009, weevil densities differed among varieties and were significantly lower on the cultivar "Jefferson." In 2011, weevil densities were reduced significantly in shallow-flooded plots compared to deep-flooded plots. Significant reductions in weevil numbers by chlorantraniliprole seed treatments, even at application rates 5 fold lower than commercially recommended rates, demonstrated the potential to reduce application rates of this highly potent larvicide. These latter results suggest that future studies on the relationship between chlorantraniliprole seed treatment rate and weevil fitness are warranted.

Evaluation of alfalfa inter-seeding effect on bahiagrass baleage fermentation and lactating Holstein performance.

BACKGROUND: Previous research indicates that bahiagrass may be successfully conserved as baleage, but nutritive value is typically low for lactating dairy cows. The purpose of this study was to determine the effect of adding modest amounts of alfalfa forage (22%), achieved by inter-seeding alfalfa into an existing bahiagrass pasture, on baleage nutritive value and lactation performance of Holstein cows. Forage treatments employed were monoculture bahiagrass baleage (MBB; negative control), bahiagrass-alfalfa mixture baleage (BAB) and conventional corn silage (CCS; positive control). Thirty six mid lactation Holstein cows [34.8 ± 5.8 kg 3.5% fat-corrected milk and 112 ± 19 d in milk (DIM)] were stratified according to milk yield and DIM and assigned randomly to 1 of 3 forage treatments. Cows were trained to Calan feeding gates and were offered a common CCS-based TMR in a 10-d covariance period followed by a 42-d treatment feeding period. RESULTS: The BAB contained more protein and less NDF than MBB (12.6 vs 10.3% CP and 71.8 vs 76.6% NDF). Diet DMI was similar for MBB and BAB (19.5 vs 21.6 kg/hd/d), but cows consumed more of the CCS diet (25.5 kg/hd/d) than either baleage-based diet. Cows offered BAB tended to produce more milk than cows offered MBB based TMR (28.4 vs 26.1 kg/hd/d), but both baleage diets generated less milk than CCS-based diets (33.1 kg/hd/d). Milk composition was similar across diets except for milk protein concentrations which were higher for CCS than either MBB or BAB diets; however, milk urea nitrogen (MUN) was lowest for cows fed CCS diets. Cow BW gain was higher for BAB than MBB implying that a portion of the higher energy contributed by the alfalfa was being used to replenish weight on these mid lactation cows. CONCLUSIONS: Data from this study indicate that alfalfa inter-seeded in bahiagrass sod that produces BAB with as little as 22% alfalfa may improve nutritive value compared to monoculture bahiagrass baleage and marginally improve lactation performance of Holstein cows. However, the CCS diet was vastly superior to either MBB or BAB-based diets for milk production.

On confidence intervals for within-subjects designs.

Confidence intervals (CIs) for means are frequently advocated as alternatives to null hypothesis significance testing (NHST), for which a common theme in the debate is that conclusions from CIs and NHST should be mutually consistent. The authors examined a class of CIs for which the conclusions are said to be inconsistent with NHST in within-subjects designs and a class for which the conclusions are said to be consistent. The difference between them is a difference in models. In particular, the main issue is that the class for which the conclusions are said to be consistent derives from fixed-effects models with subjects fixed, not mixed models with subjects random. Offered is mixed model methodology that has been popularized in the statistical literature and statistical software procedures. Generalizations to different classes of within-subjects designs are explored, and comments on the future direction of the debate on NHST are offered.

The difference between t and z and the difference it makes.

Tests of the null hypothesis for comparisons involving sample means use the t test when the conditions of the z test cannot be met. The 2 tests have different rationales and can lead to different conclusions regarding significance. In the present study, the authors compared the properties of t and z in simulation runs. The differences in the results are a result of fluctuations in the t test sample variances that do not exist in the z test, and those differences lead to differences in designating the significance of comparisons.