1.
Anal Biochem
; 151(2): 403-8, 1985 Dec.
Article
in English
| MEDLINE
| ID: mdl-4096378
ABSTRACT
A simple manual method for the routine analysis of glucose, fructose, mannose, galactose, and sucrose in plant material is described. The technique is demonstrated using various seeds known to form part of the diet of the bullfinch (Pyrrhula pyrrhula L.), a pest of commercial orchards in southeast England. The method involves the overnight extraction of the sugars with 62.5% aqueous methanol, followed by the conversion of the individual sugars to gluconate 6-phosphate (or, in the case of galactose, to galactono-gamma-lactone) by specific enzymes, and their determination spectrophotometrically.
Subject(s)
Carbohydrates/analysis , Plant Extracts/analysis , Fructose/analysis , Fruit , Galactose/analysis , Galactose Dehydrogenases , Glucose/analysis , Glucose-6-Phosphate Isomerase , Glucosephosphate Dehydrogenase , Glycoside Hydrolases , Hexokinase , Mannose/analysis , Mannose-6-Phosphate Isomerase , Spectrophotometry, Ultraviolet/methods , Sucrose/analysis , beta-Fructofuranosidase
2.
Bull Environ Contam Toxicol
; 30(3): 373-7, 1983 Mar.
Article
in English
| MEDLINE
| ID: mdl-6850124
Subject(s)
Birds/blood , Esterases/blood , Animals , Birds/genetics , Blood Protein Electrophoresis , Esterases/genetics , Female , Isoelectric Focusing , Male , Sex Factors
3.
Ecotoxicol Environ Saf
; 4(1): 1-16, 1980 Mar.
Article
in English
| MEDLINE
| ID: mdl-6991242