ABSTRACT
Antitumor activity of ultralow doses of cytostatic doxorubicin was studied on BDF1 mice with Lewis lung carcinoma. The preparation was injected intraperitoneally in single doses of 10(-5), 10(-10), 10(-15), and 10(-20) M on the next day after tumor inoculation. The effect of ultralow doses was compared with that of a standard therapeutic dose of doxorubicin (8 mg/kg, 1.4 x 10(-3) M). Doxorubicin in ultralow doses produced an antitumor effect comparable with that induced by the preparation in standard doses. On day 12 after administration of doxorubicin in ultralow and standard doses, tumor size in mice did not exceed 20% of the control level.
Subject(s)
Antineoplastic Agents/therapeutic use , Doxorubicin/therapeutic use , Animals , Antineoplastic Agents/administration & dosage , Carcinoma, Lewis Lung/drug therapy , Dose-Response Relationship, Drug , Doxorubicin/administration & dosage , Mice , Neoplasms, Experimental/drug therapySubject(s)
DNA Methylation , Nitric Oxide/physiology , Animals , Kinetics , Male , Oxidation-Reduction , Rats , Rats, WistarABSTRACT
The level in urine of 8-hydroxy-2'-deoxyguanosine (8-oxodG) is the standard biochemical marker of endogenous oxidation of DNA in humans and animals. A new method for assay of 8-oxodG in mouse and rat urine was developed. A modified method for solid phase extraction of this substance was developed. The baseline levels of 8-oxodG and 8-oxoG was measured in control rats and the effects of various compounds added to the diet or removed from the diet were assessed. The contribution of iron and copper ions to endogenous oxidation of DNA is close to 50%. Among the tested microelements (Zn, Cr, Se), the effect of selenium was the highest and depended on the duration of the selenium-deficient diet. Carcinogens including 20-methylcholanthrene and caffeic acid and a substituted quinone significantly enhanced guanine oxidation of rat DNA.
