ABSTRACT
MicroRNA375 (miR375) serves an important role in cancer development and growth. However, little is known about the role of miR375 in the regulation of oral squamous cell carcinoma (OSCC) metastasis and invasion. The present study measured the expression levels of miR375 in Tca8113, UM2, UM1 and CAL27 cell lines, using reverse transcriptionquantitative polymerase chain reaction. The results demonstrated that miR375 expression levels were significantly reduced in UM1 and CAL27 (highly metastatic) compared with Tca8113 and UM2 (less aggressive) OSCC cell lines. Furthermore, it was revealed that overexpression of miR375 suppressed the migration and invasion of UM1 cells. Based on a luciferase reporter assay, plateletderived growth factorA (PDGFA) was identified as a direct target gene of miR375. Additionally, overexpression of PDGFA significantly reversed the effect of miR375 on cell migration and invasion in UM1 cells. These data demonstrated that miR375 suppressed OSCC cell migration and invasion by targeting PDGFA, which may be a potential therapeutic target for the treatment of OSCC.
