ABSTRACT
A higher incidence of chromosomal instability in the infertile population is widely recognized. An increased level of micronuclei has been shown to be a marker of chromosome damage. Therefore, micronuclei frequencies were assessed in cytokinesis-blocked lymphocytes of 130 patients (65 couples) with idiopathic infertility or with two or more spontaneous abortions, and 30 healthy fertile donors (15 couples). The frequency of micronucleated cells in the cohort with reproductive failure and healthy controls averaged 14.95+/-6.04 per 1000 and 10.60 +/-2.57 per 1000 (P<0.0001), respectively. When micronuclei frequency sums in particular couples (male + female) were analyzed in the same order, identical statistical significance was reached (P<0.0001). We found no effect of age or sex on micronuclei frequency. In summary, the cytokinesis-blocked micronuclei assay revealed increased micronucleus frequency in couples with infertility or two or more spontaneous abortions, suggesting a possible role of chromosomal instability in reproductive failure.
Subject(s)
Abortion, Habitual/genetics , Infertility, Female/genetics , Infertility, Male/genetics , Micronuclei, Chromosome-Defective , Adult , Cell Division/drug effects , Cells, Cultured , Chromosome Breakage , Cohort Studies , Cytochalasin B/pharmacology , Female , Humans , Karyotyping , Lymphocytes/drug effects , Male , Micronucleus Tests , Middle Aged , Pregnancy , Reference ValuesABSTRACT
Antitumor effect of N-9-[2-(phosphonomethoxy) ethyl]-2,6-diaminopurine (PMEDAP) was studied in an in vivo model of s.c. transplanted Sprague-Dawley (SD/cub) rat T-cell lymphomas. Three individual SD/cub neoplasias (SD10/96, SD14/97, SD1/90) of different phenotypes were used. During the treatment, survival of the rats, increase of lymphoma mass, and DNA fragmentation detected by APO/BRDU kit, as well as Bcl2 and p53 protein expression, were followed. The study gives evidence of the positive therapeutic effect of PMEDAP in two of the three tested lymphomas, SD10/96 and SD14/97. Slowly growing SD1/90 lymphoma differs from the others in a uniform karyotype with trisomy of chromosome 11, CD4- immunophenotype, heterogeneous cellular morphology and constitutive expression of p53 protein found in some neoplastic cells. Thus, the diverse anticancer efficacy of PMEDAP treatment among SD/cub lymphomas could be associated with the different phenotypes of individual neoplasias.
