[The combined action of drinking mineral water and low-intensity electromagnetic radiation under the immobilization stress conditions (an experimental study)].

The present study carried out on white male rats in experiments with the use of biochemical, radioimmunological, and electron- microscopic methods. It was shown that the combined treatment with potable mineral water (MV) and low-intensity electromagnetic radiation (LIEMR) of ultrahigh frequency (power density less than 1 pW/cm2, the frequency about 1000 MHz) facilitated the activation of metabolic and intracellular regenerative processes in the liver and testes. One of the advantages of the combined application of MV and LIEMR over the single-factor treatment manifested itself as the weakening of stress reactions, the increase in the frequency of the plastic processes, and the more harmonious development of different forms of intracellular regeneration. The results of the study provide a deeper insight ino the mechanisms underlying the combined actions of drinking mineral water and low-intensity electromagnetic radiation; also, they justify the application of these factors for the protection of the reproductive system and the entire body from stress-induced disorders.

[The application of low-intensity electromagnetic radiation under immobilization stress conditions (an experimental study)].

The experiments carried out on outbred male white rats with the use of optical, electron-microscopic, biochemical, and radioimmunological methods have demonstrated that the application of low-intensity electromagnetic radiation (LI-EMR) with a flow density of 1 mcW/cm2 and a frequency of around 1,000 MHz both in the primary prophylaxis regime and as the therapeuticpreventive modality arrested the development of post-stress disorders in the rat testicles, liver, and thymus; moreover, it promoted activation of the adaptive, preventive, and compensatory processes. The data obtained provide a rationale for the application of low-intensity electromagnetic radiation to protect the organism from negative effects of stressful factors.

[Comparative analysis of the methods for the estimation of the biological age].

The present article was designed to report the results of comparative analysis of the methods for the estimation of the biological age based on the measurement of a number of anthropometric and functional characteristics in the relatively healthy persons at the age varying from 30 years to 75 years. The study involved 277 individuals (143 women and 134 men). All the patients underwent clinical, biochemical, hormonal and instrumental examination. The following two methods were used to determine the biological age of the participants of the study: the ontogenetic anthropometrical method (L.M. Belozerova, 1999) and the Kiev method (V.P. Voytenko, 1984). These mathematical models are based on a specific set of calculation techniques for each anthropometric and instrumental parameter and approximately equally reflect the mismatch between the calendar and biological age; however, they are different in the degree of correlation with the disturbances of metabolism, enzymatic maintenance of metabolic processes, and other parameters. By way of example, the method of L.M. Belozerova for the calculation of the anthropometric biological age largely correlates with the insulin resistance index, blood concentrations of aldosterone, triglycerides and glucose, whereas the results obtained by the method of V.P. Voytenko show stronger correlation with the activity of alkaline phosphatase, the ceruloplasmin level and self-estimation of the patients' health.

Immunorehabilitating effect of ultrahigh frequency electromagnetic fields in immunocompromised animals.

We observed immunorehabilitation effects of ultrahigh frequency electromagnetic fields (microwaves) in immunocompromised animals. It was shown that microwave irradiation of the thyroid gland area could abolish actinomycin D- and colchicine-induced immunosuppression and did not affect immunosuppression caused by 5-fluorouracil. These findings suggest that changes in the hormonal profile of the organism during microwave exposure can stimulate the processes of transcription and mitotic activity of lymphoid cells.

[The application of drinking mineral water in combination with zinc and silicon in rats undergoing immobilization stress].

The experiments carried out on male rats have demonstrated that the primary prophylactic application of drinking sulfate mineral water (MW) in combination with such microelements as zinc and silicon results in a more pronounced decrease of the magnitude of alterations in the liver, thymus, and blood hormonal spectrum under the action of immobilization stress than the application of mineral water alone. This effect is believed to be due to the activation of the organisms' adaptive reactions that enhances its resistance to stress.

[Prevention of early post-stress disorders in rat testicles under effect of drinking sulfate mineral water containing zinc and silicium].

Experiments performed on outbred male rats have demonstrated that consumption of drinking sulfate mineral water containing microelements (zinc and silicium) considerably slows down the development of stress-induced pathological changes in the testicles compared with the action of pure mineral water containing no admixtures. This finding confirms the important role of zinc and silicon in combination with the ion-salt base of mineral water in the enhancement of the efficacy of the adaptive and protective reactions in the testicles during the early post-stress period.

[The application of trans-auricular low-frequency electrical puncture for the treatment of arterial hypertension].

Clinical studies involving 67 patients with arterial hypertension (AH) have demonstrated that a single course of trans-auricular low-frequency electrical puncture produced a marked stress-relieving effect that was manifested as the decreased anxiety level, normalization of neurohumoral regulation of arterial pressure and central hemodynamics, improved tolerance of controlled psycho-emotional loading, beneficial effect on cerebral blood circulation, lipid and electrolyte metabolism. In addition, the proposed therapeutic modality resulted in substantial reduction of arterial pressure. Taken together, these observations give reason to consider it as a promising tool for the treatment of patients presenting with arterial hypertension.

[Combined effect of musically-modulated electrical current and mineral drinking water from Khadyzhensky spring in experimental atherosclerosis]. / Sochetannoe deistvie muzykal'no-modulirovannogo élektricheskogo toka i pit'evoi vody Khadyzhenskogo mestorozhdeniia pri éksperimental'nom ateroskleroze.

Male rats with experimental atherosclerosis drank mineral water (Khadyzhensky spring) and were exposed to music-modulated electric current. This combined treatment showed synergism of physical (current) and balneological (mineral water) factors providing lipolytic, antioxidant, stress-limiting and antiinflammatory intravascular effects and recovery of microcirculatory processes.

[Adaptive reactions of rat testicular endocrine cells exposed to mineral drinking waters after radiation exposure]. / Adaptivnye reaktsii éndokrinnykh kletok semennikov drys pri deistvii pit'evykh mineral'nykh vod posle radiatsionnogo oblucheniia.

Early after irradiation (2 Gy) white male rats were given sulfate mineral water, sulfate mineral water with silicon, iodine-containing mineral water (iodine concentration 30 mg/l). It was found that sulfate mineral water alone or with silicon reduces the number of endocrine Leidig's cells, inhibits their functional activity, lowers blood level of testosterone. These effects were not observed after intake of iodine-containing mineral water.

[Interference therapy in combined treatment of patients with chronic nonspecific salpingoophoritis]. / Iterferentsterapiia v kompleksnom lechenii bol'nykh khronicheskim nespetsificheskim sal'pingooforitom.

Neurotropic interference therapy (NIT) was studied as monotherapy and in combination with iodine-bromide water in 50 patients with chronic nonspecific salpingoophoritis. NIT was found effective in the above disease as it improved clinical picture, reproductive function, psychoemotional condition of the patients. The addition of iodine-bromide water to NIT potentiates and prolongs neurotropic, endocrine, analgetic and anti-inflammatory effects of NIT.

[Experimental study of the role of essential trace elements of mineral waters in prevention of atherosclerosis risk factors]. / Uchastie éssentsial'nykh mikroélementov mineral'nykh vod v ustranenii faktorov riska ateroskleroza v éksperimente.

Hormonal and lipid spectra of the blood serum; antioxidant, biosynthetic, microcirculatory and elastic-inhibitory activity in the myocardium and liver; proliferative and genetic activity in the thymus; microcirculation and destruction in rats with atherosclerosis drinking Khadyzhenskaya mineral water and solution of natural macro- and trace elements in the spring water (temarox) in two dilutions. It was found that elimination of atherosclerosis risk factors in rats was most complete in a course temarox drinking in dilution 1/8000.

[The physiological and physicochemical mechanisms of the therapeutic and prophylactic actions of a musically modulated electrical current in experimental atherosclerosis]. / Fiziologicheskie i fiziko-khimicheskie mekhanizmy lechebnogo i profilakticheskogo deistviia muzykal'no-modulirovannogo élektricheskogo toka pri éksperimental'nom ateroskleroze.

In experimental atherosclerosis it is shown that frontomastoid course application of music-modulated electric current prevents oxidant modification of low density lipoproteins in the myocardium, cerebral cortex and liver. This antioxidant effect eliminates one of the principal risk factors of atherosclerosis.

[Recovery processes in the cerebral cortex, myocardium and thymus of rats with experimental atherosclerosis exposed to low-frequency electromagnetic fields on the head]. / Vosstanovitel'nye protsessy v kore golovnogo mozga, miokarde i timuse krys s éksperimental'nym aterosklerozom pri vozdeistviiakh nizkochastotnymi élektromagnitnymi poliami na golovu.

Studies of animals with experimental sclerosis has shown that a course of 10 procedures of alternative magnetic field (AMF) (50 Hz, 30 mT, 3 min daily) promotes partial recovery of the lipid spectrum and corrects vasomotor-metabolic disturbances in the cerebral cortex, myocardium and thymus caused by atherosclerosis. Combination of AMF with constant magnetic field in the same regime and location does not produce a hypolipidemic effect in atherosclerotic animals and this, in combination with increased vascular permeability may aggravate the condition. Activated microcirculation, antioxidant and antiproteinase effects in activation of biosynthetic processes in the cerebral cortex reflect inhibition in the CNS in this combined effect and create conditions for a hypotensive effect.

[Activity of the genome of cardiomyocytes as an indicator of the development of adaptive changes in the myocardium following exposure of the central nervous system to electromagnetic fields]. / Aktivnost' genoma kardiomiotsitov kak pokazatel' razvitiia adaptivnykh izmenenii v miokarde pri vliianii élektromagnitnykh polei na tsentral'nuiu nervnuiu sistemu.

Methods of cardiomyocyte nuclei isolation from the myocard homogeneous mixture, and of cardiomyocyte genome activity estimation were elaborated. In the experiments with hyperlipoproteidemic rats, cardiomyocyte genome activity was shown to reflect the primary adaptive changes in the myocard, and to serve a reliable index of their influence on the CNS regulatory centres exposed to electromagnetic field, which is used for hyperlipoproteidemia treatment. The cardiomyocyte genome activity was used to distinguish between three types of development of adaptive reactions in the myocard.

[The biological action of a musically modulated electric current]. / Biologicheskoe deistvie muzykal'no-modulirovannogo élektricheskogo toka.

The authors suggest to use music-modulated electric current as an adequate physiologic stimulator and stress-limiting factor as it appeared to enhance functional ability of the central nervous system and activate biosynthesis in the tissues supporting active adaptive reactions.

[The combined action of infrared radiation and permanent and alternating magnetic fields in experimental atherosclerosis]. / Kombinirovannoe deistvie infrakrasnogo izlucheniia, postoiannogo i peremennogo magnitnykh polei pri éksperimental'nom ateroskleroze.

Paravertebral exposure to infrared radiation (0.87 micron, 5 mW) and permanent magnetic field in combination with one- and two-semiperiodic alternative magnetic fields (50 Hz, 15-30 mT) was studied in respect to the action on adaptive reactions in animals with experimental atherosclerosis. Complex consisting of infrared radiation, permanent magnetic field and one-semiperiodic pulse alternative magnetic field was most effective in restoration of vasomotor-metabolic and immune disturbances accompanying development of atherosclerosis.

[The transcerebral use of an impulse current in experimental alimentary hypercholesterolemia]. / Transtserebral'noe primenenie impul'snogo toka pri alimentarnoi giperkholesterinemii v éksperimente.

25 rats with alimentary hypercholesterolemia induced by diet rich in cholesterol and mercazoline were exposed to frontomastoid impulse current (1000 Hz, 0.75 mA). The treatment promoted normalization of lipid and protein metabolism in the myocardium and liver, of thyroid hormones and antiproteinase activity of the serum. Positive trends were noted in the activity of thymocyte nuclei and lipid peroxidation in the cerebral cortex.

[Experimental research on the physicochemical action of impulse currents when applied transcerebrally]. / Eksperimental'noe issledovanie fiziko-khimicheskogo deistviia impul'snykh tokov pri transtserebral'nom primenenii.

Thymocyte function and lipid peroxidation in cell membranes of the cerebral cortex and the myocardium of 72 rats were examined to study the stress-limiting mechanism of 10 and 1000 Hz impulse current effect. The additional measurements were as follows: rat and thymus weight, their proportional relations, thymocyte nuclear population, 11--OCS, thyroid hormones (T3, T4), plasma testosterone and insulin, myocardial and cerebral cortical protein. The animals were immobilized for 6 days in individual tight cages where they had food and water. Antioxidant and antistress effects of impulse currents were revealed at definite regimens of their application: 10 Hz stress limiting action was more potent when applied to stressed animals, but 1000 Hz current proved to be an effective prophylactic procedure.