ABSTRACT
The anti-inflammatory properties of non-modified fullerene C60 (FC60) by adjuvant arthritis in Wistar rats have been studied. It was shown that the intraperitoneal introduction of FC60 (50 ng) reveals an anti-inflammatory and chondroprotective actions in the phase of systemic manifestation of adjuvant arthritis. The effect was carried out by limitation of inflammation of damaged limb, normalization of body weight, the decrease in body temperature. Introduction of FC60 promote the reduction of leukocyte level, the erythrocyte sedimentation rate, concentrations of sialic acids and the ceruloplasmin levels, processes degeneration of cartilaginous tissues of the joint of rats. It has been concluded that the therapeutic effectiveness of non-modified FC60 in experimental adjuvant arthritis is comparable with the action of water-soluble forms of fullerenes. The results substantiate the future investigations of non-modified FC60 for design of therapeutic agents for treatment of rheumatoid arthritis in clinics.
Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Arthritis, Experimental/drug therapy , Cartilage/drug effects , Fullerenes/pharmacology , Knee Joint/drug effects , Animals , Arthritis, Experimental/immunology , Arthritis, Experimental/pathology , Blood Sedimentation/drug effects , Body Temperature/drug effects , Body Weight/drug effects , Cartilage/immunology , Cartilage/pathology , Ceruloplasmin/antagonists & inhibitors , Inflammation/prevention & control , Injections, Intraperitoneal , Knee Joint/immunology , Knee Joint/pathology , Leukocytes/drug effects , Leukocytes/immunology , Leukocytes/pathology , Male , Rats , Rats, WistarABSTRACT
The aim of this study was to assess the influence of fullerene C60 on lipid peroxidation (POL) and antioxidant protection during the induction of the immune response to heteroantigen. Balb/c mice were immunized intraperitoneal (i.p.) with sheep erythrocytes for the primary immunization. Water dispersion of fullerene C60 was injected i.p. once at the dose 50 ng to mice on first, third and sixth days after immunization. During immune response, the increment ofmalonic dialdehide (MDA) was enhanced in liver, kidneys and heart tissues. Fullerene C60 induced POL during the latent phase of immune response, but inhibited this process during progression of immune response. Activities of superoxide dismutase (SOD) and catalase in liver and spleen tissues were induced after injection of fullerene C60 to intact mice. After immunization, high level of activity of antioxidant enzymes and low level of organs mass factor were determined. Injection of fullerene C60 reduced the activities of SOD and catalase in spleen tissues. The results of our study indicate that fullerene C60 can display positive effect on POL processes and antioxidant enzymes activity which is probably due to membrane's stabilization action or the ability of fullerene C60 to bind free radicals independently.
Subject(s)
Antigens, Heterophile/immunology , Antioxidants/therapeutic use , Erythrocytes/immunology , Fullerenes/therapeutic use , Lipid Peroxidation/drug effects , Animals , Antigens, Heterophile/administration & dosage , Antioxidants/administration & dosage , Catalase/metabolism , Free Radicals/antagonists & inhibitors , Free Radicals/metabolism , Fullerenes/administration & dosage , Heart/drug effects , Injections, Intraperitoneal , Kidney/drug effects , Kidney/immunology , Kidney/metabolism , Lipid Peroxidation/immunology , Liver/drug effects , Liver/immunology , Liver/metabolism , Male , Malondialdehyde/analysis , Mice , Mice, Inbred BALB C , Sheep , Spleen/drug effects , Spleen/immunology , Spleen/metabolism , Superoxide Dismutase/metabolismABSTRACT
We investigated the population prevalence of polymorphisms of Arg753Gln TLR2gene, and Asp299Gly, Thr399Ile TLR4 genes among people living in the Poltava region, as well as data communication polymorphisms with the presence of diseases caused by urogenital infections. The group of population control was a random sample of residents of the Poltava region (n = 299). The group of patients with urogenital diseases included 156 people. Genotyping of these groups TLR2 Arg753Gln polymorphism and the gene TLR4 Asp299Gly, Thr399Ile was performed using PCR and subsequent restriction analysis. Statistically significant association of allele A ofgene TLR2 (p = 0.0018) and allele G of gene TLR4 (p = 0.085) with the presence of urogenital diseases was confirmed.
