Blusher mushroom (Amanita rubescens Pers.): A Study of Mercury Content in Substrate and Mushroom Samples from Slovakia with Respect to Locality and Developmental Stages.

The edible mushroom Amanita rubescens Pers., regularly collected and consumed in Slovakia, was assessed for health risk due to the mercury content in its fruiting body parts. For this purpose, 364 both from the soil/substrate and mushroom samples from 40 localities in Slovakia were evaluated. At the same time, 21 samples of 7 developmental stages of the fruiting body of A. rubescens were taken in the Zakýlske pleso locality. The total mercury content in the soil and mushroom samples was determined using an AMA-254 analyzer. The contamination factor (Cf) and index of geoaccumulation (Igeo) were used to detect the level of soil pollution by mercury. The ability of A. rubescens to accumulate mercury from the soil environment was evaluated using the bioconcentration factor (BCF), and the distribution of mercury in the mushroom body was evaluated using the translocation quotient (Qc/s). To determine the health risks resulting from mushroom consumption, the percentages of provisional tolerable weekly intake (%PTWI) and target hazard quotient (THQ) were used. The obtained results have confirmed serious content of mercury soil pollution, especially in former mining areas, where the situation is alarming from a health risk point of view. Consumption of A. rubescens was found to be risky, not only in former mining areas, but higher values of mercury were also detected in other parts of Slovakia. Evaluation of the developmental stages of the fruiting body of A. rubescens showed that the highest bioconcentration factor was determined at developmental stage no. VI for caps with a value of 2.47 mg kg-1 and developmental stage VII for stipes with a value of 1.65 mg kg-1 DW.

Mercury in scarletina bolete mushroom (Neoboletus luridiformis): Intake, spatial distribution in the fruiting body, accumulation ability and health risk assessment.

In the present work, we focused on two aspects of mercury (Hg) bioconcentration in the above-ground parts of Neoboletus luridiformis. In the first part, we monitored the bioconcentration potential of individual anatomical parts of a particular fruiting body and evaluated the obtained data by the spline interpolation method. In the second part, we focused on assessing the mercury content in 378 samples of N. luridiformis and associated samples of substrates from 38 localities with different levels of Hg content in Slovakia. From the obtained data of Hg content in samples of substrate and fungi, we evaluated ecological indicators (geoaccumulation index - Igeo, contamination factor - Cf a potential ecological risk - PER), bioconcentration indicators (bioconcentration factor - BCF; cap/stipe quotient - Qc/s) and health indicators (percentage of provisional tolerable weekly intake - %PTWI a target hazard quotient - THQ). Based on the Hg distribution results, the highest Hg content was found in the tubes & pores (3.86 mg/kg DW), followed by the flesh of cap (1.82 mg/kg DW). The lowest Hg content was in the stipe (1.23 mg/kg DW). The results of the BCF values indicate that the studied species can be included in the category of mercury accumulators. The results of the ecological indices representing the state of soil pollution pointed out that two localities (Malachov and Nizná Slaná) stood apart from all monitored localities and showed a state of an extremely disturbed environment. This fact was also reflected in the values of Hg content in the fruiting bodies of the studied mushroom species. In the case of the consumption of mushrooms from these localities, it can be stated that long-term and regular consumption could have a negative non-carcinogenic effect on the health of consumers. It was confirmed by the %PTWI (Malachov: 57.8%; Nizná Slaná: 53.2%) and THQ (Malachov: 1.11 Nizná Slaná: 1.02). The locality Cacín-Jelsovec is interesting from the bioconcentration characteristics point of view, where the level of environmental pollution was the lowest (Hg content in the soil was below the background value) compared to other localities, however, the THQ value was the highest (1.29).

Mercury Content in Three Edible Wild-Growing Mushroom Species from Different Environmentally Loaded Areas in Slovakia: An Ecological and Human Health Risk Assessment.

Three edible mushroom species (Imlera badia, Boletus subtomentosus, Xerocomellus chrysenteron) sampled in 60 different localities of Slovakia were evaluated to determine health risks (associated with mercury content) arising from their consumption. Total content of mercury in soil and mushroom samples was determined by an AMA-254 analyzer. Soil pollution by mercury was evaluated by contamination factor (Cf), pollution load index (PLI), and geoaccumulation index (Igeo). Bioaccumulation factor (BAF), translocation factor (Qc/s), provisional tolerably weekly intake (%PTWI), estimated daily intake (EDI), and target hazard quotient (THQ) were used to describe and compare uptake and transition abilities of mushrooms, and the health risk arising from mushroom consumption. Mercury content in soil significantly influences the content of mercury in mushroom fruiting body parts. Caps and stipes of Boletus subtomentosus were found to be the best mercury accumulator. According to the EDI, consumption of the evaluated mushroom species represents a serious threat for adults and children. The lowest values of THQ were found for Xerocomellus chysenteron.

Evaluation of Soil and Ambient Air Pollution Around Un-reclaimed Mining Bodies in Nizná Slaná (Slovakia) Post-Mining Area.

Thirty soil samples were taken, and the same number of moss (Dicranum scoparium) and lichen (Pseudevernia furfuracea) bags were exposed to detect environmental pollution in the former mining area Nizná Slaná. Soil and ambient air are influenced by hazardous substances, which leak from old mining bodies due to insufficient or completely missing reclamation. The total content of the risk elements (As, Cd, Co, Cu, Fe, Hg, Mn, Ni, Sb, Se, Pb, Zn) was determined in soil, moss, and lichen samples and in the bodies of Leccinum pseudoscabrum. Biological (soil enzymes-urease, acid phosphatase, alkaline phosphatase, fluorescein diacetate (FDA), ß-glucosidase) and chemical properties (pH) were determined in soil samples. Contamination factor (Cf), degree of contamination (Cd), pollution load index (PLI), and enrichment factor (EF) were used for soil and relative accumulation factor (RAF) for air quality evaluation. Contamination factor values show serious pollution by Cd, Fe, Hg, and Mn. Pollution load index confirmed extremely high pollution almost at all evaluated areas. Soil enzymes reacted to soil pollution mostly by decreasing their activity. Mosses and lichens show differences in the accumulation abilities of individual elements. Regular consumption of L. pseudoscabrum would provide the dose of Cd and Hg below the limit of provisional weekly intake. Based on the bioaccumulation index (BAF) values, L. pseudoscabrum can be characterized as an Hg accumulator.

Open mining pits and heaps of waste material as the source of undesirable substances: biomonitoring of air and soil pollution in former mining area (Dubnik, Slovakia).

Moss and lichen bag technique (Dicranum sp., Hypnum sp., Polytrichum sp., Hypogymnia physodes) and activity of soil enzymes (urease, acid and alkaline phosphatase, fluorescein diacetate, ß-glucosidase) were used as bioindicators of air and soil pollution in Dubník former mining area (East Slovakia). Ten open mining pits and 8 heaps of waste material were chosen for the research purposes. Contamination factor (Cf), degree of contamination (Cd), and pollution load index (PLI) were used to evaluate the level of soil pollution and relative accumulation factor (RAF) expressed the level of air pollution by risk elements (As, Cd, Cu, Fe, Mn, Ni, Sb, Pb, Zn). Based the degree of contamination results, the study area was polluted by individual elements in the following order: Fe>Cd>As>Pb>Sb>Zn>Cu>Ni>Mn. The highest values of Mn and Ni and lower values of urease were determined in open mining pits comparing heaps of waste material. The results of PLI index confirmed extreme pollution at all sampling sites. Considering the average RAF values showed the decrease of accumulation abilities of evaluated taxa in the following order: Dicranum sp.>Hypnum sp.>Hypogymnia physodes>Polytrichum sp.

A case study of soil food web components affected by Fallopia japonica (Polygonaceae) in three natural habitats in Central Europe.

This study determined the effect of the invasive plant Fallopia japonica on soil physical properties, microbial respiration, microbial biomass carbon content, enzymatic activities, and soil nematode communities. We established in total 30 plots in three natural habitats (forest, grassland, wetland) that were either uninvaded or mostly monospecifically invaded by F. japonica. The soil physical and microbial properties differed among the investigated plots, but the differences were observed to be non-significant between the invaded and the uninvaded plots. Non-metric multidimensional scaling based on nematode species diversity indicated that the total number of identified nematode species and their abundance were higher in the uninvaded compare to the invaded plots. Negative effect of F. japonica on omnivores, plant parasites, and root-fungal feeder nematodes was confirmed by their lower abundance in the invaded compared to the uninvaded plots. In the invaded plots, we also confirmed lower Maturity and Channel index, but higher Enrichment index. Our results thus indicated that the invasive plant F. japonica could affect nematode communities, more than physical or microbial properties, regardless of habitat.This study determined the effect of the invasive plant Fallopia japonica on soil physical properties, microbial respiration, microbial biomass carbon content, enzymatic activities, and soil nematode communities. We established in total 30 plots in three natural habitats (forest, grassland, wetland) that were either uninvaded or mostly monospecifically invaded by F. japonica. The soil physical and microbial properties differed among the investigated plots, but the differences were observed to be non-significant between the invaded and the uninvaded plots. Non-metric multidimensional scaling based on nematode species diversity indicated that the total number of identified nematode species and their abundance were higher in the uninvaded compare to the invaded plots. Negative effect of F. japonica on omnivores, plant parasites, and root-fungal feeder nematodes was confirmed by their lower abundance in the invaded compared to the uninvaded plots. In the invaded plots, we also confirmed lower Maturity and Channel index, but higher Enrichment index. Our results thus indicated that the invasive plant F. japonica could affect nematode communities, more than physical or microbial properties, regardless of habitat.

Activity of the soil enzymes and moss and lichen biomonitoring method used for the evaluation of soil and air pollution from tailing pond in Nizná Slaná (Slovakia).

The surrounding of the poorly maintained tailing ponds is endangered by the toxic substances and represents a serious risk for the health of the local population. The aim of the study was to determine the soil pollution by the hazardous elements (As, Cr, Cd, Cu, Fe, Mn, Ni, Pb, Zn) around the tailing pond using contamination factor (Cf), degree of contamination (Cd) and pollution load index (PLI). The health and the condition of soil were evaluated by soil enzyme activity (urease, acid and alkaline phosphatase, florescein diacetate, and ß-glucosidase). The spreading of the airborne hazardous elements from the body of the tailing pond was evaluated by moss and lichen bag technique and relative accumulation factor was used for the result expression. Cd, Fe, and Mn in soils reached above the limit values at all sampling sites. According to the degree of contamination (Cd), the soils at the sampling area were very high contaminated by As, Cd, Cu, Fe, Mn, Ni, and Pb. The most part of the assessed area was according to the PLI values extremely polluted. The air pollution was the most serious around the tailing pond, but serious levels of some hazardous elements were determined also in the remote distances.

Impact of nitrogen additions on soil microbial respiration and temperature sensitivity in native and agricultural ecosystems in the Brazilian Cerrado.

Soil microbial respiration (SMR1) is a major flux of CO2 from terrestrial ecosystems into the atmosphere, which depends on several abiotic factors, including soil temperature and nutrient availability. A nutrient of great interest in soil ecology is nitrogen (N) because of its use in agriculture and an expected increase of depositions in soils. Models on the relationship between SMR and temperature may be able to describe the effects of N addition on the temperature sensitivity of soil respiration (Q102). Hence, this study aimed to investigate the effects of N addition on SMR and Q10 values in soils of native Brazilian Cerrado (NC3), and of an agricultural ecosystem (AE4) cultivated over the past 17 years. SMR was stimulated by N additions (100 and 200 mg N kg dwt soil-1) in samples of Cerrado sites depending on the land use type and temperature assessed. The addition of 200 N kg dwt soil-1 to NC showed higher SMR values at 25 °C compared to soil without N input, whereas the same happened in AE at 30 °C. Overall, NC presented higher Q10 values than AE. N inputs increased Q10 in the Cerrado sites. In NC, the highest Q10 value occurred when 200 mg N kg dwt soil-1 was added to soil, whereas in AE the greatest value was observed for a lower dose (100 mg N kg dwt soil-1). This study shows that N additions to tropical soils alter Q10 values depending on dose and land use. These are important findings to compare the fragility of pristine and cultivated Brazilian Cerrado ecosystems in the perspective of climate change.

Accumulation and environmental risk assessment of heavy metals in soil and plants of four different ecosystems in a former polymetallic ores mining and smelting area (Slovakia).

Heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in soils and plants of four different ecosystems (forest, grassland, agro and urban ecosystem) at different distances from the source of the pollution were analyzed in order to assess and compare soil contamination in the various ecosystems and determine the potential accumulation of plants depending on the place they inhabit. Correlation relationships among heavy metals in soils differ depending on the ecosystem, and between soil and plant, the heavy metals showed significant correlation for Cu, Mn, Ni, Pb and Zn. Contamination factor (Cf), degree of contamination (Cd) and pollution load index (PLI) were used in order to determine the level of environmental contamination of the study area. All studied ecosystems were rated as moderately contaminated (except agroecosystem, which was found as low contamination ecosystem) according to Cd and extremely polluted according to PLI. The highest pollution in both cases was found in urban ecosystem, and Cd, Cu and Fe were determined as the biggest pollutants.