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1.
Vestn Otorinolaringol ; (5): 58-60, 2000.
Article in Russian | MEDLINE | ID: mdl-11051861

ABSTRACT

Chlamydia trachomatis DNA was detected by PCR in 12 patients with continuously recurrent catarrhal pharyngolaryngitis. These included 9 males and 3 females whose age ranged 16 to 72 years. All the patients were found to have degrees II-III alpha- and g-INF deficiencies. The interferon inducer amixine in combination with sumamed was used in the treatment of patients with chlamydial laryngitis. After treatment, there was a significant improvement in two thirds of patients. The pain syndrome was relieved, hoarseness diminished, and the laryngoscopic and microlaryngostroboscopic picture and interferon status became normal. Control studies showed no Ch. trachomatis in this group of patients. Despite positive changes in the course of the disease, the control study again detected Ch. trachomatis in 4 patients, which required repeated treatment courses.


Subject(s)
Chlamydia Infections/diagnosis , Chlamydia Infections/drug therapy , Interferon Inducers/therapeutic use , Laryngitis/diagnosis , Laryngitis/drug therapy , Tilorone/therapeutic use , Adolescent , Adult , Aged , Chlamydia Infections/microbiology , Chlamydia trachomatis/isolation & purification , Female , Humans , Laryngitis/microbiology , Male , Middle Aged , Severity of Illness Index
2.
Vestn Akad Med Nauk SSSR ; (4): 56-61, 1990.
Article in Russian | MEDLINE | ID: mdl-2368483

ABSTRACT

New principles of management, planning, and finance of medical science with the aim to increase its efficacy are discussed. The transfer from financing the scientific administration to financing research is taken into account. The technology of expert examination is analysed as this is a basis for adopting decisions in the management of medical science.


Subject(s)
Health Planning Technical Assistance/trends , Health Planning/trends , Health Services Research/trends , Health Services Research/economics , Humans , USSR
4.
Vopr Virusol ; 27(4): 488-90, 1982.
Article in Russian | MEDLINE | ID: mdl-7135930

ABSTRACT

Comparative studies of various factors of cellular immunity in human chronic herpetic stomatitis (CHS) in periods of relapses and remission of the infection revealed no significant changes in specific cellular immune response, blasttransformation of lymphocytes to herpes simplex virus antigen. At the same time, the indices of nonspecific cellular responsiveness: the rosette-forming activity of T-lymphocytes and the level of leukocyte interferon were markedly reduced in the period of recurrence of the infection and increased as remission developed. The role of immune factors in the pathogenesis of herpes relapses is discussed.


Subject(s)
Stomatitis, Herpetic/immunology , Adolescent , Adult , Child , Child, Preschool , Chronic Disease , Humans , Immunity, Cellular , Immunity, Innate , Interferon Type I/immunology , Lymphocyte Activation , Middle Aged , Recurrence , Rosette Formation , Stomatitis, Herpetic/etiology
5.
Vopr Virusol ; 27(3): 323-7, 1982.
Article in Russian | MEDLINE | ID: mdl-7123914

ABSTRACT

A comparative study of various factors of humoral immunity in human chronic herpes stomatitis (CHS) in periods of relapses and remission of the infection showed the exacerbations of the disease to occur in the presence of complement-fixing (CF) and virus-neutralizing (VN) antibodies in the blood serum the titres of which moderately rose with the progress of the infection. A relapse of the disease occurs during marked decline in the content of secretory IgA and true secretory Sc-IgA, serum and secretory IgG, low levels of serum interferon, and a small rise in titres of 19S specific CF antibodies. Remission comes as these values become normal. Although specific immunological changes do occur in CHS, they are not very marked, whereas the factors of nonspecific host resistance change considerably in close correlation with the periods of relapse and remission of the infection.


Subject(s)
Stomatitis, Herpetic/immunology , Adolescent , Adult , Antibody Formation , Child , Child, Preschool , Chronic Disease , Humans , Immunity, Innate , Immunoglobulin A/analysis , Immunoglobulin A, Secretory/analysis , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Middle Aged , Neutralization Tests , Recurrence , Stomatitis, Herpetic/etiology
7.
Vopr Virusol ; (2): 161-5, 1980.
Article in Russian | MEDLINE | ID: mdl-6247843

ABSTRACT

A preparation of herpes simplex virus type 2 (HSV-2) nucleocapsid (NC) was obtained after treatment of intracellular virus with detergents followed by centrifugation in linear sucrose density gradient. Electron microscopic analysis revealed polymorphism of HSV-2 NC population. Three main types of capsid structures were found: empty capsids, NC containing various amounts of nucleoid material, and NC with complete nucleoid. Morphological heterogeneity correlated with heterogenous sedimentation profile of the total NC preparation of labeled virus represented by light, intermediate, and heavy structures. Specific blasttranformation tests with lymphocytes from rabbits immunized with HSV-2 NC demonstrated differences in the primary and secondary cellular immune responses. The secondary cellular immune response was characterized by a more rapid and strong increase in BTT values than the primary immune response.


Subject(s)
Antigens, Viral/immunology , Immunity, Cellular , Lymphocyte Activation , Simplexvirus/immunology , Animals , Capsid/immunology , Capsid/isolation & purification , Immunization Schedule , Nucleic Acids/analysis , Nucleic Acids/immunology , Nucleic Acids/isolation & purification , Rabbits , Simplexvirus/isolation & purification , Simplexvirus/ultrastructure , Virion/immunology , Virion/isolation & purification
8.
Vopr Virusol ; (3): 277-82, 1979.
Article in Russian | MEDLINE | ID: mdl-462927

ABSTRACT

The influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (HSV-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-MCA), was studied. Solid tumors developed in 20-MCA-treated mice on the side of the carcinogen administration within 81--89 days. The simultaneous administration of 70 intracerebral LD50 of HSV-2 subcutaneously into a hind leg pad and 0.1 mg 20-MCA subcutaneously into the inguinal area was accompanied by a synergistic action of the two factors. The number of tumors in the animals treated with HSV-2 in combination with 20-MCA at all intervals of observation was 1.5--2.5 times greater than in mice treated with 20-MCA alone. Mice weighing 10--12 g were more sensitive to the synergistic effect of HSV-2 and 20-MCA, whereas animals weighing 25--30 g were more sensitive to the carcinogenic effect of 20-MCA alone. No tumors developed in mice infected with HSV-2 alone by 167 days (the observation period). Mice weighing 10--12 g were found to be more sensitive to the fatal effect of HSV-2 and HSV-2 plus 20-MCA, particularly in the first 2--3 weeks after inoculation. Six months after inoculation with HSV-2 the virus was isolated from posterior root ganglia of the sacral and lumbar spinal cord by cocultivation of the ganglia with human embryo skin-muscle tissue cells.


Subject(s)
Carcinogens , Disease Models, Animal , Herpes Simplex/complications , Neoplasms, Experimental/etiology , Animals , Chronic Disease , Ganglia, Spinal/microbiology , Methylcholanthrene , Mice , Neoplasm Transplantation , Time Factors , Virus Cultivation
9.
Vopr Virusol ; (2): 142-8, 1979.
Article in Russian | MEDLINE | ID: mdl-219611

ABSTRACT

The results of comparative studies on concentration and purification of herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2) by ficol density gradient centrifugation are presented. A two-phase distribution of extracellular HSV was established in phycol density gradient centrifugation: in zones with density of 1.110-1.114 and 1.088-1.085 g/ml. The effectiveness of purification of HSV preparations recovered from the corresponding gradient zones was determined by electron microscopy and quantitation of the contaminating cellular (radioactive) proteins in virus purification from a mixture of the culture fluid from infected cultures and the culture fluid from uninfected labeled human embryo skin-muscle tissue cultures (HESM) and a mixture of unlabeled extracellular HSV and a homogenate of labeled uninfected HESM cultures. In HSV purification from the virus-containing culture fluid the amount of cellular proteins was shown to decrease 500-fold in 150-fold virus concentration. In purification of extracellular HSV from the mixture with cell homogenate the amount of cellular proteins decreased 70- and 100-fold for HSV-2 and HSV-1, respectively. The infectious virus yield in phycol gradient centrifugation of a precipitate obtained by the addition of polyethylene glycol-6000 to the culture fluid for HSV-1 was 34.7% (in titrations in HESM cultures) and 38.4% (by intracerebral inoculation of mice weighing 5-6 g), and for HSV-2 20.2% and 26.3%, respectively.


Subject(s)
Simplexvirus/isolation & purification , Animals , Centrifugation, Density Gradient , Chemical Phenomena , Chemistry, Physical , DNA, Viral/isolation & purification , Ficoll , Mice , Simplexvirus/analysis , Simplexvirus/pathogenicity , Viral Proteins/isolation & purification , Virion/analysis
10.
Vopr Virusol ; (6): 690-4, 1977.
Article in Russian | MEDLINE | ID: mdl-74909

ABSTRACT

Isopicnic centrifugation in cesium chloride gradient produces sufficiently homogeneous fractions of large (42--45 nm) and small (20--22 nm) particles detectable in sera from patients with viral hepatitis and donors with antigenemia. In addition to morphological criteria, differences between these particles were indicated by a greater buoyant density (1.24 g/cm3), higher sensitivity to lower pH values and a lower resistance between. No antigenic determinats inherent in small particles could be found in large particles.


Subject(s)
Hepatitis B Surface Antigens/ultrastructure , Hepatitis B/blood , Centrifugation, Isopycnic , Epitopes , Hepatitis B/microbiology , Hepatitis B Surface Antigens/isolation & purification , Humans
11.
Vopr Virusol ; (3): 364-9, 1977.
Article in Russian | MEDLINE | ID: mdl-71790

ABSTRACT

Comparative studies of the biological properties, antigenic structure and ultrastructure of influenza A viruses isolated in the USSR, A/New Jersey/8/76 and A/swine/31 were carried out. Antigenic differences between A/USSR/02/76 and A/swine/31 strains as well as A/New Jersey/8/76 strain were found in the hemagglutination-inhibition and neuraminidase activity-inhibition tests. These differences were confirmed by serological examination of human sera.


Subject(s)
Influenza A virus , Antigens, Viral , Epitopes , Hemagglutination Inhibition Tests , Hemagglutination, Viral , Humans , Influenza A virus/immunology , Influenza A virus/ultrastructure , Microscopy, Electron , Military Medicine , Neuraminidase/metabolism , New Jersey , Species Specificity , USSR
13.
Vopr Virusol ; (2): 196-202, 1977.
Article in Russian | MEDLINE | ID: mdl-898896

ABSTRACT

The effect of exogenous leukocyte interferon on the course of chronic oncornavirus infection of lymphoblastoid J-96 cells chronically producing type B virus was studied. By means of radio-isotope analysis and electron microscope examinations it was shown that upon long-term passage of the cells (18-34 passages) in the presence of interferon (10 units/ml) the process of virion formation in the cells was inhibited 2.0-4.4-fold and virus budding to a lower extent (1.6-2.7-fold). Interferon exerted no inhibiting effect on the formation of intracytoplasmic virions of type A. The employment of KC-test showed the oncornavirus produced by J-96 cells in the presence of interferon to have retained its biological activity, being able to induce synthesis of the indicator KC cells. Examination of the cell membranes by electrophoresis in polyacryl amide gel showed that interferon contributed to accumulation of glycoproteins with high molecular weights (115 000, 100 000 and 68 ooo daltons) in this cell fraction. Simultaneously the experimental cells were found to have 2-3-fold increased amount of inter-species group-specific antigen of Mason-Pfizer virus. The mechanism of action of interferon on multiplication of J-96 cell oncornavirus is discussed.


Subject(s)
Interferons/pharmacology , Oncogenic Viruses/drug effects , Cell Line , Cell Membrane/metabolism , Glycoproteins/metabolism , Interferons/therapeutic use , Microscopy, Electron , Virus Diseases/drug therapy , Virus Diseases/metabolism , Virus Replication/drug effects
14.
Vopr Virusol ; (2): 164-8, 1977.
Article in Russian | MEDLINE | ID: mdl-70886

ABSTRACT

The evidence has been obtained indicating the presence of the antigen of leukocyte leukosis virus (LLV) in specimens from patients with leukosis. Immunofluorescence tests with rabbit immune serum to LLV revealed specific fluorescence of the cytoplasm of cells in 19 out of 32 bone marrow punctates and leukocytes in 10 out of 12 blood smears from patients with acute leukosis and chronic myeloleukosis. In these tests, the difference from analogous control materials (from donors, patients with polyarthritis, reticulosclerodermia, mveloma, etc. is highly statistically significant. By the CFT, antibody to LLV-infected cells were detected in 60.8% of patients with leukosis and 18.5% of donors. When examined by the gel precipitation test with the appropriate test-systems LLV antigen was found to contain no group-specific antigen of murine leukosis virus or antigen of oncornaviris D from HEp-2 continuous cell line.


Subject(s)
Bone Marrow Cells , Bone Marrow/microbiology , Leukemia/microbiology , Leukocytes/microbiology , Retroviridae , Acute Disease , Animals , Complement Fixation Tests , Epitopes , Fluorescent Antibody Technique , Humans , Leukemia Virus, Murine/immunology , Leukemia, Lymphoid/microbiology , Leukemia, Myeloid/microbiology , Oncogenic Viruses/immunology , Rabbits/immunology , Retroviridae/immunology
16.
Vopr Virusol ; (6): 660-3, 1976.
Article in Russian | MEDLINE | ID: mdl-193302

ABSTRACT

The evidence of mutual stimulation of herpes simplex virus (HSV) and oncornaviruses (OV) reproduction was obtained. HSV titers in mice pre-infected with Rauscher leukosis virus (RLV) were 2.25-3.0 lg LD50 higher than in mice of the same batch but without RLV infection. The continuous HEp-2 culture chronically infected with oncornaviruses was found to be more sensitive to the cytopathic effect of HSV strains, types 1 and 2, and in the culture fluid HSV accumulated to a greater titer than in primarily trypsinized culture of chick embryo fibroblasts. Electron microscope examinations of the continuous J-96 cell culture chronically infected with OV and HSV simultaneously revealed an 8.3-fold increase in the number of oncornavirus particles as compared to the control culture, infected with OV alone.


Subject(s)
Oncogenic Viruses , Rauscher Virus , Simplexvirus , Virus Diseases/microbiology , Virus Replication , Animals , Cell Line , Cyclophosphamide/pharmacology , Lymphocytic choriomeningitis virus , Mice , Mice, Inbred BALB C
17.
Biull Eksp Biol Med ; 82(8): 995-8, 1976 Aug.
Article in Russian | MEDLINE | ID: mdl-799941

ABSTRACT

A continuous cell line of human spleen has been obtained. The methods of seeding large numbers of cells and prolonged cultivation of the culture played an important role in isolation of this cell line; both methods have contributed to the creation of conditions for gradual reconstruction of the cellular metabolism in the absence of which the cells wound not be able to get adapted to contiuous cultivation. As the authors failed to obtain a contiuous cell line from a single cell colony the method of "shaking" was applied. The culture was obtained only in case of a 1% PHA solution was added to the nutrient medium.


Subject(s)
Spleen/cytology , Cell Line , Cells, Cultured , Culture Media , Cytological Techniques , Cytopathogenic Effect, Viral , Humans , Lectins/pharmacology , Time Factors
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