ABSTRACT
A field chick infectious bronchitis virus (IBV) was isolated from the pathological material on chick embryos. The nucleotide sequence of the S1 gene was determined and comparatively analyzed with some sequences of this gene of foreign and Russian vaccine strains and isolates. A cross-neutralization test using sera to various IBV seroptypes was performed. The isolate was shown to antigenically differ from the reference strains. Bioassay was carried out, by using one-day chicks and the immunogenic properties of the virus were investigated.
Subject(s)
Coronavirus Infections/veterinary , Disease Outbreaks , Infectious bronchitis virus/classification , Poultry Diseases/epidemiology , Poultry Diseases/virology , Animals , Antibodies, Viral/immunology , Cells, Cultured , Chick Embryo , Chickens/virology , Coronavirus Infections/epidemiology , Coronavirus Infections/virology , Genes, Viral/genetics , Hemagglutinins, Viral , Infectious bronchitis virus/genetics , Infectious bronchitis virus/immunology , Infectious bronchitis virus/isolation & purification , Infectious bronchitis virus/pathogenicity , Membrane Glycoproteins/genetics , Molecular Sequence Data , Neutralization Tests , Russia/epidemiology , Sequence Analysis, Protein , Species Specificity , Spike Glycoprotein, Coronavirus , Viral Envelope Proteins/genetics , VirulenceABSTRACT
A field isolate of Newcastle disease virus (NDV) was isolated in the Russko-Vysotskaya poultry farm, Leningrad region. Within four days after infection, the isolate caused 100% mortality in 60-day-old susceptible chickens. The HA titer of the allantoic fluid samples collected after one passage in SPF-chicken embryos was 1:512, and it reacted only with the NDV specific antiserum in HI test. Intracerebral pathogenicity index and mean embryo death time were 1.97 and 49 hours, respectively. The isolate has the amino acid sequence of the protease cleavage site of the fusion protein F0 (112R-R-Q-R-R-F117), which is similar to that in the velogenic strains of NDV. Therefore, it was concluded that the virus isolated in this work was an ethiological agent of the ND outbreak in this poultry farm.
Subject(s)
Disease Outbreaks/veterinary , Newcastle Disease/epidemiology , Newcastle disease virus/isolation & purification , Poultry/virology , Animals , Catalytic Domain/genetics , Chick Embryo , Chickens , Endopeptidases/metabolism , Hemagglutination Tests , Newcastle Disease/virology , Newcastle disease virus/genetics , Newcastle disease virus/metabolism , Polymerase Chain Reaction , RNA, Viral/analysis , Russia/epidemiology , Viral Fusion Proteins/genetics , Viral Fusion Proteins/metabolismABSTRACT
Experience gained in the hygienic training of those who are engaged at public catering enterprises and grocery stores in different regions of the Russian Federation is presented. Hygienic training courses have been set up in the State Sanitary and Epidemiological Surveillance centers. Programs for training workers at public catering enterprises and grocery stores have been approved by the board and the Russian Federation State Sanitary and Epidemiological Surveillance Committee and the Trade Committee of the Russian Federation.
