ABSTRACT
Adenoviruses isolated from pigs (RO-KL; SA3; RO; and R10) and the prototypic strain Compton 25R, belonging to the 1st serologic group, are responsible for the formation of intranuclear bodies of the Cowdry-A type, while the virus Ad5 strain (isolated from humans) cause the formation of another variant of nuclear inclusions of the Cowdry-B type known as Central mass. All virus strains propagate in primary cell cultures of pig kidney and calf and rabbit kidney, calf testis, and in the stable cell line PK-15, showing negligible variations. Resistance and stability experiments have demonstrated that the investigated strains posses physico-chemical and morphologic properties that are characteristic of the adenoviruses. The virus strain isolated in Bulgaria, and the strains isolated in Hungary can be classified in the IVth serologic group of swine adenoviruses. The study of serum samples taken in different regions of Bulgaria and Hungary in order to establish adenovirus antibodies have revealed that in about 20.5 per cent of the swine sera in Hungary, and in 19 per cent, on an average, of the sera taken in Bulgaria there are adenovirus antibodies.
Subject(s)
Adenoviridae/isolation & purification , Swine/microbiology , Adenoviridae/ultrastructure , Animals , Antibodies, Viral/analysis , Cells, Cultured , DNA, Viral/analysis , Serotyping , Swine/immunology , Virus Cultivation , Virus ReplicationSubject(s)
Cattle Diseases/microbiology , Herpesviridae Infections/veterinary , Herpesviridae/isolation & purification , Animals , Bronchopneumonia/microbiology , Bronchopneumonia/veterinary , Cattle , Cells, Cultured , Chemical Phenomena , Chemistry, Physical , Cytopathogenic Effect, Viral , Guinea Pigs , Herpesviridae/immunology , Herpesviridae/pathogenicity , Herpesviridae Infections/microbiology , Immune Sera , Microscopy, Electron , Rabbits , Rats , Serotyping , Virology/methods , Virus CultivationABSTRACT
Kidneys and sera of calves from various farms and primary kidney tissue cultures were tested for mycoplasma and acholeplasma contamination. Altogether 24 strains belonging to Mycoplasma arginini and Acholeplasma axanthum were isolated from tissue cultures, kidneys and sera. The same species were detected from lungs and peribronchial lymph nodes of calves, together with A. laidlawii, A. modicum and M. bovirhinis species. There was a close correlation between the mycoplasma content of tissue cultures, sera and kidneys and the clinical picture observed in the farm, as well as between the quality of tissue cultures and the mycoplasma content of organs, sera and tissue cultures.
Subject(s)
Blood/microbiology , Kidney/microbiology , Mycoplasma/isolation & purification , Animals , Cattle , Cells, Cultured , Cytopathogenic Effect, Viral , Lung/microbiology , Lymph Nodes/microbiology , Male , Mycoplasma/classification , Mycoplasma/growth & development , Mycoplasma/immunology , TestisABSTRACT
Acholeplasma axanthum sp. was isolated from the lung of swine with catarrhal pneumonia. Clinical symptoms of respiratory disease, gross and histological lesions of pneumonia, as well as serological response were produced by intranasal inoculation of ;miniature pigs' with the supernatant of lung suspension containing Acholeplasma axanthum and by a 48 hr. broth culture of the strain.A similar picture of disease was observed in animals held in contact with the animals inoculated with untreated lung suspension. Acholeplasma axanthum was isolated from the nasal cavity, lung and peribronchial lymph nodes 7-41 days after inoculation. No lesions were observed after inoculation of pigs with the supernatant of lung suspension pretreated with oxytetracycline or chloroform, and no successful isolation of Acholeplasma axanthum could be achieved after this treatment.
