ABSTRACT
Antioxidant activity of a pharmaceutical substance hypocard was compared with activity of nitromalic acid and well-known agents nicorandil and Mexidol. The ability of these substances to inhibit spontaneous and oxidant-induced LPO process in rat brain homogenate was analyzed. The mechanisms of these effects were studied. The antioxidant properties of hypocard manifested in the inhibition of Fe(II)-induced LPO were significantly more pronounced in comparison with Mexidol and nicorandil.
Subject(s)
Antioxidants/metabolism , Animals , Brain/drug effects , Brain/metabolism , Lipid Peroxidation/drug effects , Male , Nicorandil/pharmacology , Oxidative Stress/drug effects , Oxidative Stress/physiology , Picolines/pharmacology , RatsABSTRACT
It has been showed that the introduction of nitrocompounds (as nitic oxide donors) in to the compositions of cyclophosphamide and hydroxamic acids for curing animals having leukemia P-388 increased duration of life by 290%. Thereby 40% of animals have recovered. The therapeutic dose cyclophosphamide have been reduced by 6 times.
Subject(s)
Antineoplastic Agents/pharmacology , Cyclophosphamide/pharmacology , Hydroxamic Acids/pharmacology , Leukemia P388/drug therapy , Nitrates/pharmacology , Animals , Drug Synergism , Female , Male , Mice , Survival Analysis , Treatment OutcomeABSTRACT
The purpose of the investigation was to study age-related changes in reaction of the hypothalamic-pituitary-adrenal axis and erythrocyte antioxidant enzyme system in response to acute psychoemotional stress in non human primates. Ten young (6-8 years) and ten old (20-26 years) healthy female rhesus monkeys were subjected to acute moderate psycho-emotional stress (two hours squeeze cage restraint) at 15:00 h. Plasma cortisol (F), activities of superoxide dismutase (SOD), glutathione peroxidase, gluthatione reductase (GR), gluthatione-S-transferase, and lipid peroxidation products (TBARS) in erythrocytes were measured before stress and 30, 60, 120, 240 min and 24 h after beginning of the stress. In young monkeys SOD activity decreased in response to the stress while it increased in the half of old monkeys. Young animals also demonstrated essentially higher increase in plasma F level and GR activity in response to the restraint, in comparison with old monkeys. Level of TBARS did not change in response to the stress in young animals and significantly increased in old monkeys. The age-related alterations in F, SOD, and GR stress responsiveness lead to activation of peroxide oxidation of lipids that may be considered as an important factor of aging damage of erythrocyte functioning and reliability of oxygen transport to tissues under stress conditions.
Subject(s)
Aging/physiology , Hypothalamo-Hypophyseal System/physiology , Lipid Peroxidation/physiology , Oxidation-Reduction , Pituitary-Adrenal System/physiology , Stress, Psychological/metabolism , Animals , Antioxidants/metabolism , Erythrocytes/metabolism , Female , Glutathione Peroxidase/blood , Glutathione Reductase/blood , Glutathione Transferase/blood , Hydrocortisone/blood , Macaca mulatta , Superoxide Dismutase/blood , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
Cell transformation by the complete and the incomplete virus SV20 was investigated. To compare the oncogenic properties of human adenoviruses, two types of cell systems--the primary rat embryonic cell culture and the primary rat kidney cell culture--were used. The dependence of DNA concentration on transformation frequency was investigated. With the incomplete virus SV20, the transformation efficiency was more than that with the complete virus (16 and 1-3 percent, resp.). In addition, it has been shown that the primary rat kidney cell culture is more sensitive to transformation than the primary rat embryonic cells culture. The new-born Sirian hamsters being inoculated with virus SV20 DNA, the growth of a tumor being registered in the point of injection 120 days afterwards.
