ABSTRACT
Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs' size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1â¯ppm, within the incubation time of 2â¯h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.
Subject(s)
Aniline Compounds , Anti-Infective Agents , Blood Cells/metabolism , Copper , DNA Damage , Escherichia coli/growth & development , Nanocomposites , Staphylococcus aureus/growth & development , Aniline Compounds/chemistry , Aniline Compounds/pharmacology , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Blood Cells/cytology , Copper/chemistry , Copper/pharmacology , Drug Evaluation, Preclinical , Humans , Nanocomposites/chemistry , Nanocomposites/therapeutic useABSTRACT
The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353nm] and [370-410nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells' surfaces.
Subject(s)
Antifungal Agents/pharmacology , Candida albicans/drug effects , Hyphae/drug effects , Metal Nanoparticles/chemistry , Silver/pharmacology , Spores, Fungal/drug effects , Antifungal Agents/chemistry , Antifungal Agents/metabolism , Candida albicans/growth & development , Candida albicans/metabolism , Candida albicans/ultrastructure , Hyphae/growth & development , Hyphae/metabolism , Hyphae/ultrastructure , Kinetics , Optical Imaging/methods , Silver/chemistry , Silver/metabolism , Spores, Fungal/growth & development , Spores, Fungal/metabolism , Spores, Fungal/ultrastructure , Synchrotrons , Tryptophan/chemistry , Ultraviolet RaysABSTRACT
Gold-polyaniline (Au-PANI) nanocomposite was prepared using a simple interfacial polymerization method, performed in an immiscible water/toluene biphasic system using tetrachloroaurate, AuCl4(-) as an oxidant. The formation of Au nanoparticles (AuNPs) or Au-PANI nanocomposite can be controlled to a certain degree by varying the ratio of initial Au(+) and aniline concentrations. Under optimal condition (HAuCl4/aniline ratio is 1:2), green dispersion of Au-PANI nanocomposite is produced in aqueous phase, whose morphology, structure and physicochemical properties are investigated in details. The nanocomposite shows granular morphology with mostly rodlike AuNPs embedded in polymer. It was found that polyaniline in the composite is in the conducting emeraldine salt form, containing high amount of Au (28.85 wt %). Furthermore, the electrical conductivity of the nanocomposite was found to be four-fold higher than that of the polymer itself. In addition, the nanocomposite powder, isolated from the as-prepared aqueous dispersion, can later be easily redispersed in water and further used for various applications. Moreover, the obtained Au-PANI nanocomposite showed excellent electrocatalytic performance toward the electrochemical oxygen reduction reaction (ORR), with high ORR onset potential and good selectivity. This makes it a promising candidate for a new class of Pt-free ORR catalyst.
ABSTRACT
This study explores different mechanisms of antimicrobial action by designing hybrid nanomaterials that provide a new approach in the fight against resistant microbes. Here, we present a cheap copper-polyaniline (Cu-PANI) nanocomposite material with enhanced antimicrobial properties, prepared by simple in situ polymerization method, when polymer and metal nanoparticles are produced simultaneously. The copper nanoparticles (CuNPs) are uniformly dispersed in the polymer and have a narrow size distribution (dav = 6 nm). We found that CuNPs and PANI act synergistically against three strains, Escherichia coli, Staphylococcus aureus, and Candida albicans, and resulting nanocomposite exhibits higher antimicrobial activity than any component acting alone. Before using the colony counting method to quantify its time and concentration antimicrobial activity, different techniques (UV-visible spectroscopy, transmission electron microscopy, scanning electron microscope, field emission scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectrophotometry, and inductively coupled plasma optical emission spectrometry) were used to identify the optical, structural, and chemical aspects of the formed Cu-PANI nanocomposite. The antimicrobial activity of this nanocomposite shows that the microbial growth has been fully inhibited; moreover, some of the tested microbes were killed. Atomic force microscopy revealed dramatic changes in morphology of tested cells due to disruption of their cell wall integrity after incubation with Cu-PANI nanocomposite.