ABSTRACT
Lactobacilli have been barely studied in cows. We proposed isolate and characterize lactic acid bacteria from dairy cows as compared to those raised for meat production and elucidate the presence of strains with evident probiotic employment's potential. For this, isolation and quantification of LAB mainly lactobacilli were realized from vaginal cattle samples in MRS medium. Each selected microorganism was then briefly characterized. The MATH method was employed using hexadecane, xilene an toluene as solvent. According to the hydrophobic characteristics, strains were classified into three categories: high (71-100%), medium (36-70%) and low (0-35%). Hydrogen peroxide qualitative production was studies too, lactobacilli were streaked onto an MRS agar plate containing 5 mg of 3,3',5,5'-tetramethylbenzidine and 0.20 mg of horseradish peroxidase. Twenty-one sampled cows (78%) were positive for lactic acid microflora, 12 belonging to the dairy group and 17 of the meat group. Total LAB counting including dairy and meat cows were log 2,41 CFU/ml. Of overall identified strains, an 83% corresponded to lactobacilli. Most strains belonged to the heterofermentative facultative group (75%), with L. plantarum as the most frequent specie. The highest proportion of isolated vaginal strains (69%) had low hydrophobicity, the LAB with highest hydrophobic characteristics (3 strains) were found only in meat cows. In the qualitative evaluation of H(2)O(2) production, a positive reaction was observed in 13 of 29 strains (45%). The role of lactobacilli in vaginal microbiota is limited, and therefore the present work is interesting in incorporate knowledge of normal microflora of progesteronic healthy cows, in this case in production animals. The isolation and characterization data obtained are consistent in consider the study of particular strains with great potential in the development of a probiotic for production cows.
Subject(s)
Cattle/physiology , Estrous Cycle , Lactobacillus/isolation & purification , Vagina/microbiology , Animals , Female , Hydrogen Peroxide/metabolism , Hydrophobic and Hydrophilic Interactions , Lactobacillus/chemistry , Lactobacillus/metabolism , PregnancyABSTRACT
OBJETIVOS: Nos propusimos comprobar si los factores hormonales tienen influencia en la actividad y evolución del Lupus Eritematoso Sistémico (LES), determinando los niveles séricos de estradiol, testosterona, FSH y de la concentración de receptores estrogénicos en células monoclonales de sangre periférico(CMSP). METODOLOGIA: Se seleccionaron 22 pacientes con diagnóstico de LES basados en los criterios de la ARA 1982, del sexo femenino culminando sólo 10 de ellos, la investigación. Se realizó registro clínico, inmunoserológico, niveles séricos de estradiol, testosterona, FSH y concentración de receptores estrogénicos en CMSP tanto para los pacientes como para los controles. El análisis estadístico se realizó mediante la "t" de Student y el índice de correlación lineal de Pearson. RESULTADOS: En el 80 por ciento de los pacientes se observó Anyti-Adn elevados. Factores del Complemento disminuidos en el 90 por ciento y complejos inmunes circulantes elevados en la totalidad de los casos. La creatinina sérica fue normal contrastando con el hallazgo de nefritis lúpica en el 100 por ciento. Los estrógenos séricos elevados significativamente (p<0,01), testosterona disminuida (p<0,01), FSH sin diferencia estadística en ambos grupos (p<0,5). No hubo correlación entre el estradiol, testosterona en relación a la concentración de receptores estrogénicos en CMSP e igualmente no se constató correlación entre la concentración de receptores estrogénicos y los índices de actividad de la enfermedad. CONCLUSION: La determinación de los factores del coplemento viene a ser índices precisos de actividad, superiores a los anticuerpos Anti-Adn bicatenario. En todo paciente lúpico, existe nefropatía. Proponemos que las pacientes con LES, tienen a poseer estradiol y por otra parte, la testosterona disminuida en relación a la población normal. En cuanto a la concentración de receptores de estrógeno en CMSP, no se encontró diferencia entre los grupos. No pareciera relacionarse los niveles elevados de estradiol con la actividad de la enfermedad, ni existir correlación entre la actividad estrogénica y la lúpica.
Subject(s)
Adolescent , Adult , Middle Aged , Humans , Female , Lupus Erythematosus, Systemic/blood , Lupus Erythematosus, Systemic/diagnosis , Receptors, Estrogen/analysisABSTRACT
Forty nine patients with carcinoma of the cervix treated with a combined method of preoperative radiation therapy (telecobalto and intracavitary) followed by extrafascial hysterectomy are presented. The average age was 39.6 years old. All tumors measured more than 4 cm, in diameter. The staging was lb = 40; lla = 6; llb = 3. The hysterectomy was done average 7.3 weeks after radiation therapy. Postreatment complications were seen in 32.4% and long term complications were found in 8.1% of the cases. After three years of follow up 91% of the patients were free of neoplastic disease. The 40% of the cases has more than three years follow up. There was 14% of total mortality which includes 10.2% of mortality by cancer. The size of the tumor (initially more than 7 cm, in diameter) and the finding of the tumor tissue in the surgical specimens were found of poor prognosis. There was significant statistical difference in survival between the groups with and without cancer cells in the uterus after hysterectomy (p < 0.01).
Subject(s)
Uterine Neoplasms/therapy , Adult , Combined Modality Therapy , Female , Follow-Up Studies , Humans , Middle Aged , Recurrence , Survival Rate , Time Factors , Uterine Neoplasms/mortalityABSTRACT
The aim of this work was to study the prevalence of hypertension in rural populations of the VIII region of Chile. Six thousand twenty four non institutionalized subjects. 14 years old or older, coming from eight randomly chosen rural communities were studied. Blood pressure was measured by trained personnel in two occasions within 30 minutes; in those classified as hypertensive, a third measurement was performed within 24 hours, eliminating alcohol intake and medications. Results show prevalence rates in the first, second and third measurement of 38.5, 30.8 and 22.8% respectively. The elimination of alcohol intake and medications decreased the prevalence in 3.9%. All the chosen communities has similar rates (19.8 to 21.2%) except Arauco, that had a significantly higher frequency of 31.9%. Zones of extreme ruralness had higher rates than small towns (23.8 vs 21.7%). There was a secular increase in the prevalence of hypertension from 4.9 in the 15 to 24 years old group to 57.8% among people 75 years old or older. Forty three percent of hypertensives were aware of such condition, 26.1% were receiving treatment and in 8.2% it was successful; all these figures were slightly better among women.
Subject(s)
Hypertension/epidemiology , Rural Health , Adolescent , Adult , Aged , Chile/epidemiology , Female , Health Knowledge, Attitudes, Practice , Humans , Male , Middle Aged , Prevalence , Rural Population , Sampling StudiesABSTRACT
We surveyed 605 households selected by conglomerate random sampling in a typical Chilean city in order to study patterns of utilization of medicinal agents. The critical item investigated drug use in the week preceding the interview. The 95% confidence limits for drug utilization were 24.1 and 26.8% and for medicinal plants 16.7 and 18.7%. Mothers (35.7%) and children (32.1%) used more drugs than adult males. Analgesics and minor tranquilizer were the drugs most frequently used, the main indication being related to respiratory diseases. Self prescription of drugs was about 30%.
