ABSTRACT
Changes of electrokinetic properties of cell nuclei and the quantity of granules of heterochromatin located near the nuclear envelope in nuclei of human buccal epithelium cells were studied under the influence of electromagnetic fields in vitro. Irradiation of cells was realized by means of a semiconductor generator of millimeter radiation (wavelength 7.1 mm, frequency 42.2 GHz), the Yav-1 apparatus for extremely high frequency therapy. It was shown that irradiation of cells induced a decrease in electric charge of native human buccal epithelium cell nuclei and an increase in chromatin condensation in nuclei. The observed effects depend on irradiation dose and individual peculiarities of donors.
Subject(s)
Cell Nucleus/radiation effects , Heterochromatin/radiation effects , Microwaves , Mouth Mucosa/radiation effects , Adult , Aged , Female , Humans , Middle Aged , Mouth Mucosa/ultrastructure , SemiconductorsABSTRACT
An original method for the determination of cell membrane permeability has been developed. Human buccal epithelium cells were treated with Indigo Carmine and the percentage of stained cells was determined. Employing this method, the age-related differences in properties of plasma membrane in cells of human buccal epithelium were demonstrated. In the group of aged donors the average level of cell stainability was higher than in the group of younger donors. These results demonstrate a decrease in outer cell membrane integrity with age.
