ABSTRACT
The technique MALDI-TOF mass spectrometry was applied using device Microflex with database MALDI Biotyper (Bruckeer Daltonics Inc.) to identify with high level of reliability 8 strains P. fulva from collection of pseudo monads isolated from clinical material in St. Petersburg. When analyzing the same strains applying technique MALDI TOF mass spectrometry using device Vitek MS (bioMerieux) these starins were wrongly identifies as P. putida. The complex of tests of common analysis was approved and proposed for control differentiation of P. fulva and P. putida. The medical significance of P. fulva was approved.
Subject(s)
Pseudomonas Infections/diagnosis , Pseudomonas putida/isolation & purification , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Bacteriological Techniques/methods , Humans , Oxidoreductases/biosynthesis , Oxidoreductases/genetics , Pigments, Biological/biosynthesis , Pigments, Biological/genetics , Pseudomonas Infections/genetics , Pseudomonas Infections/microbiology , Pseudomonas putida/genetics , Pseudomonas putida/pathogenicity , Species SpecificityABSTRACT
AIM: Complex characteristic by phenotype signs and main virulence genes of Yersinia enterocolitica strains circulating in various regions of Russian Federation. MATERIALS AND METHODS: 46 strains of Y. enterocolitica of 2 - 4 biotypes and 401 strains of Y. enterocolitica IA biotype isolated in 15 administrative territories of Russian Federation (Siberian, Far Eastern, Northwestern, Urals Federal Districts) from infected people, rodents, agricultural animals, birds, the environment were studied. Phagotyping was performed in the reference laboratory of the Pasteur Institute (Paris). All the Y. enterocolitica cultures were studied for the presence of ail, ystB and ystA genes by PCR method. Presence of virulence plasmid pYVwas determined by gel electrophoresis by T. Kieser method. RESULTS: 447 strains of Y. enterocolitica biotype 1A and 2 - 4 were studied. Most of the strains belonged to serotypes O:3; O:9; O: 5; O: 6,30; O:6,31; O:7,8. Phagotyping was performed for part of the strains. Phagotypes Xz and Xo were determined in biotype 1A strains. 2 - 4 biotype strains circulating in Siberia and the Far East were characterized by phagotype VIII, X3 that are present in other countries, and phagotype Xz that is spread only in Russia. Phagotypes IXa, IXb, II that are characteristic for strains from Canada, South Africa, Japan were not detected in Russian Federation. All the strains of 2 - 4 biotypes had ail and ystA genes. Most of the recently isolated strains had pYV. The only pathogenicity factor detected in 81.3% of biotype 1A strains including 14 strains from patients was ystB gene. These infections were accompanied by an expressed clinical symptomatology of enteritis and enterocolitis. CONCLUSION: Isolation of 1A biotype strains from patients necessitates execution of diagnostic studies of intestinal yersiniosis in patients with diagnosis "acute intestinal infection of undetermined etiology".
