Cue reduction or general cue masking do not underlie generalized chemical camouflage in pirate perch.

Avoiding detection is perhaps the ultimate weapon for both predators and prey. Chemosensory detection of predators via waterborne or airborne cues (predator-released kairomones) is a key prey adaptation in aquatic ecosystems. Pirate perch, Aphredoderus sayanus, a largely insectivorous mesopredatory fish, are considered to be chemically camouflaged because they are unavoided by all colonizing organisms tested, including treefrogs and aquatic insects, despite stronger predatory effects on target taxa than several avoided fish. To address the mechanism behind camouflage we used aquatic insect colonization as a bioassay to test (1) whether increasing pirate perch density/biomass leads to increased avoidance, and (2) whether pirate perch mask heterospecific fish kairomones. Insect abundances, species richness, and community structure showed no response to pirate perch density. Last, pirate perch did not mask the kairomones of heterospecific predatory fish. Results support the idea that fish kairomones are species-specific, and chemical camouflage is driven by a unique chemical signature that is either undetectable or has no negative associations for colonists.

Predator-specific responses and emergent multi-predator effects on oviposition site choice in grey treefrogs, Hyla chrysoscelis.

Predators affect prey through both consumptive and non-consumptive effects (NCEs), and prey typically face threats from multiple simultaneous predators. While different predators have a variety of NCEs on prey, little is known regarding effects of simultaneous multiple predators on demographic habitat selection. Demographic habitat selection is unique among NCEs, especially in discrete habitat patches; decisions directly affect both distribution and abundance of species across habitat patches, rather than simply abundance and performance within patches. Our goal was to determine strength of avoidance responses to multiple species/species combinations of predatory fish, and responses to predator richness. We assessed responses of ovipositing grey treefrogs (Hyla chrysoscelis) to three predatory fish species and substitutive combination of species. In single-species treatments, treefrogs avoided only one species, Notemigonus crysoleucas. All two-species combinations, and the three-species combination, were avoided, including the Fundulus chrysotus × Noturus phaeus combination, of which neither were avoided alone. This suggests emergent properties of multiple predators, with potential interactive effects among cues themselves or in the perception of cues by treefrogs. Our results indicate effects of multiple predators are not predictable based on individual effects, and illustrate the importance and complexity of effects of demographic habitat selection on distribution and abundance.

Exposure to glucocorticoids alters life history strategies in a facultatively paedomorphic salamander.

Polyphenisms, where two or more alternative, environmentally-cued phenotypes are produced from the same genotype, arise through variability in the developmental rate and timing of phenotypic traits. Many of these developmental processes are controlled or influenced by endogenous hormones, such as glucocorticoids, which are known to regulate a wide array of vertebrate ontogenetic transitions. Using the mole salamander, Ambystoma talpoideum, as a model, we investigated the role of glucocorticoids in regulating facultative paedomorphosis, an ontogenetic polyphenism where individuals may delay metamorphosis into terrestrial adults. Instead, individuals reproduce as aquatic paedomorphic adults. Paedomorphosis often occurs when aquatic conditions remain favorable, while metamorphosis typically occurs in response to deteriorating or "stressful" aquatic conditions. Since glucocorticoids are central to the vertebrate stress response and are known to play a central role in regulating obligate metamorphosis in amphibians, we hypothesized that they are key regulators of paedomorphic life history strategies. To test this hypothesis, we compared development of larvae in outdoor mesocosms exposed to Low, Medium, and High exogenous doses of corticosterone (CORT). Results revealed that body size and the proportion of paedomorphs were both inversely proportional to exogenous CORT doses and whole-body CORT content. Consistent with known effects of CORT on obligate metamorphosis in amphibians, our results link glucocorticoids to ontogenetic transitions in facultatively paedomorphic salamanders. We discuss our results in the context of theoretical models and the suite of environmental cues known to influence facultative paedomorphosis.

Multiple urban stressors drive fish-based ecological networks in streams of Columbus, Ohio, USA.

Integrating a network perspective into multiple-stressor research can reveal indirect stressor effects and simultaneously estimate both taxonomic and functional community characteristics, thus representing a novel approach to stressor paradigms in rivers. Using six years of data from twelve streams of Columbus, Ohio, USA, the effects of nutrients (N:P), impervious surface (%IS), and sedimentation on network properties were quantified. Variability in the strength and distribution of trophic interactions was assessed by incorporating biomass into networks. All stressors impacted some properties of network topology - linkage density (average number of links per species), connectance (fraction of all possible links realized in a network), and compartmentalization (degree to which networks contain discrete sub-webs), including synergistic interactive effects between sedimentation and stream size. We also found support for antagonistic effects between (1) sedimentation and %IS and between %IS and N:P on the weighted index mean link weight, which represents the magnitude of trophic interactions among species in a network, and (2) %IS and stream size on strength standard deviation, a measure of the distribution of total magnitude of all trophic interactions per species in a network. Overall, our results point to the potential for urban stressors such as impervious surfaces and sedimentation - alone and as interactions - to decrease network complexity, compartmentalization, and stability, likely through homogenizing habitat and limiting food resources. The observation that larger streams often buffered the negative effects of these stressors suggests that restoration and other management approaches might be most beneficial in smaller headwater streams of urban catchments.

Patch Size as a Niche Dimension: Aquatic Insects Behaviorally Partition Enemy-Free Space across Gradients of Patch Size.

Positive correlation of species richness with area is ubiquitous in nature, but the processes driving that relationship, as well as those constraining typical patterns, remain elusive. Patch size variation is pervasive in natural systems, and it is thus critical to understand how variation in patch size, as well as its potential interaction with factors like predation and isolation, affects community assembly. We crossed patch quality (fish presence/absence) with patch size to the examine effects of quality, size, and their interaction on colonization by aquatic insects. Overall, beetles favored small, fishless patches, but individual species sorted across patch size while hemipterans aggregated into large, fishless patches, producing sorting between Coleoptera and Hemiptera. Both patch size and predation risk generated significant variation in community structure and diversity. Patch size preferences for the 14 most abundant species and preeminence of species turnover in patterns of ß-diversity reinforce patch size as a driver of regional species sorting via habitat selection. Species sorting at the immigration stage plays a critical role in community assembly. Identifying patch size as a component of perceived quality establishes patch size as a critical niche dimension and alters our view of its role in assembly dynamics and the maintenance of local and regional diversity.

Predation risk and patch size jointly determine perceived patch quality in ovipositing treefrogs, Hyla chrysoscelis.

Two of the most important factors determining community structure and diversity within and among habitat patches are patch size and patch quality. Despite the importance of patch size in existing paradigms in island biogeography, metapopulation biology, landscape ecology, and metacommunity ecology, and growing conservation concerns with habitat fragmentation, there has been little investigation into how patch size interacts with patch quality. We crossed three levels of patch size (1.13 m2 , 2.54 m2 and 5.73 m2 ) with two levels of patch quality (fish presence/absence, green sunfish [Lepomis cyanellus] and golden shiners [Notemigonus crysoleucus]) in six replicate experimental landscapes (3 × 2 × 6 = 36 patches). Both fish predators have been previously shown to elicit avoidance in ovipositing treefrogs. We examined how patch size and patch quality, as well as the interaction between size and quality, affected female oviposition preference and male calling site choice in a natural population of treefrogs (Hyla chrysoscelis). Females almost exclusively oviposited in the largest fishless patches, indicating that females use both risk, in the form of fish predators, and size itself, as components of patch quality. Females routinely use much smaller natural and experimental patches, suggesting that the responses to patch size are highly context dependent. Responses to fish were unaffected by patch size. Male responses largely mimicked those of females, but did not drive female oviposition. We suggest that patch size itself functions as another aspect of patch quality for H. chrysoscelis, and serves as another niche dimension across which species may behaviorally sort in natural systems. Because of strong, shared avoidance of fish (as well as other predators), among many colonizing taxa, patch size may be a critical factor in species sorting and processes of community assembly in freshwater habitats, allowing species to behaviorally segregate along gradients of patch size in fishless ponds. Conversely, lack of variation in patch size may concentrate colonization activity, leading to intensification of species interactions and/or increased use of lesser quality patches.