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1.
Anal Biochem ; 340(2): 231-44, 2005 May 15.
Article in English | MEDLINE | ID: mdl-15840496

ABSTRACT

In previous articles [Anal. Biochem. 284 (2000) 201; J. Lipid Res. 43 (2002) 794], we reported that the GC/MS identification and quantification of nearly all constituents of glycolipids could be obtained on the same sample in a single GC/MS analysis as heptafluorobutyrate derivatives of the products liberated using acid-catalyzed methanolysis. The same type of data could be obtained on glycoproteins and proteoglycans [Biochemistry 42 (2003) 8342]. These experiments were performed on material from higher organisms, and there was no evidence that bacteria-specific constituents could also be identified and quantified. The current article reports that the GC/MS analysis of compounds liberated by acid-catalyzed methanolysis as heptafluorobutyrate derivatives allows the simultaneous qualitative and quantitative determinations of pentoses, deoxyhexoses, hexoses, hexosamines, uronic acids, Kdo, Mur, heptose, Kdn, and neuraminic acid as well as of most fatty acids (including hydroxylated fatty acids). This approach provides a way of obtaining fingerprints of bacterial constituents and quantification of the overall effect of gene inactivation or of culture conditions.


Subject(s)
Bacteria/chemistry , Fluorocarbons/chemistry , Glycoconjugates/isolation & purification , Lipids/isolation & purification , Fatty Acids/isolation & purification , Gas Chromatography-Mass Spectrometry , Hydroxy Acids/analysis , Lipids/analysis , Methanol/chemistry , Monosaccharides/isolation & purification , Muramic Acids/analysis , Polyamines/isolation & purification , Sensitivity and Specificity
2.
Anal Biochem ; 329(2): 199-206, 2004 Jun 15.
Article in English | MEDLINE | ID: mdl-15158478

ABSTRACT

C-mannosylation of Trp residue is one of the most recently discovered types of glycosylation, but the identification of these mannosylated residues in proteins is rather tedious. In a previous paper, it was reported that the complete analysis of all constituents of glycoproteins (sialic acids, monosaccharides, and amino acids) could be determined on the same sample in three different steps of gas chromatography/mass spectrometry of heptafluorobutyrate derivatives. It was observed that during the acid-catalyzed methanolysis step used for liberation of monosaccharide from classical O- and N-glycans, Trp and His were quantitatively transformed by the addition of a methanol molecule on their indole and imidazole groups, respectively. These derivatives were stable to acid hydrolysis used for the liberation of amino acids. Since monosaccharide derivatives were also stabilized as heptafluorobutyrate derivatives of O-methyl-glycosides, it was suggested that C-mannosides of Trp residues could quantitatively be recovered. Based on the analyses of standard compounds, peptides and RNase 2 from human urine, we report that C((2))-mannosylated Trp could be quantitatively recovered and identified during the step of amino acid analysis. Analyses of different samples indicated that this type of glycosylation is absent in bacteria and yeasts.


Subject(s)
Chemistry Techniques, Analytical/methods , Glycoproteins/chemistry , Tryptophan/analogs & derivatives , Tryptophan/chemistry , Bacteria/chemistry , Candida albicans/chemistry , Chromatography, Gas/methods , Spectrometry, Mass, Electrospray Ionization/methods
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