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1.
Neurology ; 72(18): 1595-600, 2009 May 05.
Article in English | MEDLINE | ID: mdl-19414727

ABSTRACT

BACKGROUND: Determination of auditory function is a fundamental part of a complete neurologic examination. Disability from permanent hearing loss is common in the general population. Current bedside auditory tests are unreliable and cumbersome. We evaluated the calibrated finger rub auditory screening test (CALFRAST) as a routine diagnostic tool. METHODS: The sound spectrum and mean peak intensities of standard finger rub were measured, as well as background noise. CALFRAST overlapped the frequency spectrum of normal speech. Patients and companions were recruited from a neurology clinic. With arms extended, two stimulus intensities were presented: strong finger rub (CALFRAST-Strong 70) and the faintest rub that the examiner could hear (CALFRAST-Faint 70). With subjects' eyes closed, each ear's CALFRAST threshold was ascertained and then compared with its audiometric measure. The normal threshold was considered to be 25 dB. Validity, reliability, and discrimination abilities were obtained using standard methods. RESULTS: Two hundred twenty-one subjects (442 ears; 58% women) were examined. Ages ranged from 18 to 88 years, with a mean of 46 years. Eighty-five subjects (39%) had some degree of hearing loss. Both specificity and positive predictive value of CALFRAST-Strong 70 were 100%. Both sensitivity and negative predictive value of CALFRAST-Faint 70 were 99%, with a negative likelihood ratio <0.1. Area under the receiver operating characteristic curve was 0.94, consistent with excellent discrimination ability. Both intrarater and interrater reliability were excellent, both kappa >0.8. Subjects' self-assessment of hearing was unreliable. CONCLUSION: The calibrated finger rub auditory screening test (CALFRAST) is simple, accurate, inexpensive, and reliable. As a routine screening tool, CALFRAST may contribute to more efficient identification of auditory impairment.


Subject(s)
Acoustic Stimulation/methods , Audiometry/methods , Fingers/physiology , Hearing Disorders/diagnosis , Mass Screening/methods , Point-of-Care Systems/trends , Adolescent , Adult , Aged , Aged, 80 and over , Audiometry/economics , Cost-Benefit Analysis , Female , Hearing Disorders/physiopathology , Humans , Male , Middle Aged , Observer Variation , Pitch Discrimination/physiology , Predictive Value of Tests , Reproducibility of Results , Young Adult
2.
Hear Res ; 192(1-2): 75-82, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15157965

ABSTRACT

The Mouse Cochlea Database (MCD) is a collection of resources that include digital images and bibliographic information on the mouse cochlea and is available at: http://mousecochlea.ccgb.umn.edu. The purpose of this communication is to report on the development of one MCD resource: the Digital Cytocochleogram. A cytocochleogram is a graphic representation of the anatomical state of the hair cells along the complete width and length of the organ of Corti. The Digital Cytocochleogram provides Internet users with a complete collection of digital images of one or more surface preparations of the mouse organ of Corti from which morphometric information can be obtained. By moving a mouse driven, screen cursor over a digital image, the location and approximate frequency region of the anatomical structure is displayed. Users can also measure the straight-line distance between any two structures on the image. The Digital Cytocochleogram resource uses two software programs, the Coordinate Finder and Viewer, which are written as CGI scripts. The Coordinate Finder program maps each digital image to an X,Y coordinate system. The total length of the organ of Corti from all tissue segments is computed using an arc-distance approximation formula, with the lateral border of the inner pillar cell headplates serving as a trace line or reference location. After all of the digital images of the tissue segments are mapped, they are placed on the MCD Website where users can use the Viewer program to view and morphometrically assess structures using a web browser. A single, complete surface preparation from a normal mouse is presently available on the MCD website. As the MCD grows, additional images of surface preparations at different magnifications from normal, mutant, and experimentally altered mouse cochleas will become available.


Subject(s)
Cochlea/anatomy & histology , Databases, Factual , Animals , Image Processing, Computer-Assisted , Internet , Mice , Models, Anatomic , Software
4.
Ann N Y Acad Sci ; 942: 493-6, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11710498

ABSTRACT

Identification of genes involved in hereditary vestibular disease is growing at a remarkable pace. Mutant mouse technology can be an important tool for understanding the biological mechanism of human vestibular diseases.


Subject(s)
Vestibular Diseases/genetics , Animals , Dyneins , Eye Movements , Humans , Immunohistochemistry , Mice , Mice, Mutant Strains , Myosin VIIa , Myosins/metabolism , Potassium Channels/genetics , Potassium Channels/physiology , Reflex, Vestibulo-Ocular , Shaker Superfamily of Potassium Channels , Vestibular Diseases/metabolism , Vestibular Diseases/physiopathology
5.
Neurobiol Dis ; 8(1): 69-77, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11162241

ABSTRACT

It is still debated to what extent the vestibular deficits in Usher patients are due to either central vestibulocerebellar or peripheral vestibular problems. Here, we determined the origin of the vestibular symptoms in Usher 1B patients by subjecting them to compensatory eye movement tests and by investigating the shaker-1 mouse model, which is known to have the same mutation in the myosin-VIIa gene as Usher 1B patients. We show that myosin-VIIa is not expressed in the human or mouse cerebellum and that the vestibulocerebellum of both Usher 1B patients and shaker-1 mice is functionally intact in that the gain and phase values of their optokinetic reflex are normal. In addition, Usher 1B patients and shaker-1 mice do not show an angular vestibuloocular reflex even though eye movement responses evoked by electrical stimulation of the vestibular nerve appear intact. Finally, we show histological abnormalities in the vestibular hair cells of shaker-1 mice at the ultrastructural level, while the distribution of the primary vestibular afferents and the vestibular brainstem circuitries are unaffected. We conclude that the vestibular dysfunction of Usher 1B patients and shaker-1 mice is peripheral in origin.


Subject(s)
Blindness/physiopathology , Deafness/physiopathology , Vestibular Diseases/etiology , Animals , Blotting, Western , Cerebellum/metabolism , Dyneins , Electric Stimulation , Eye Movements/physiology , Hair Cells, Auditory/pathology , Hair Cells, Auditory/ultrastructure , Humans , Immunohistochemistry , Mice , Mice, Neurologic Mutants , Microscopy, Electron , Myosin VIIa , Myosins/genetics , Neurons, Afferent/physiology , Neurons, Efferent/physiology , Syndrome , Vestibular Diseases/pathology , Vestibular Diseases/physiopathology
6.
Am J Otol ; 21(4): 505-9, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10912695

ABSTRACT

PURPOSE: To determine if noise damage in the organ of Corti is different in the low- and high-frequency regions of the cochlea. MATERIALS AND METHODS: Chinchillas were exposed for 2 to 432 days to a 0.5 (low-frequency) or 4 kHz (high-frequency) octave band of noise at 47 to 95 dB sound pressure level. Auditory thresholds were determined before, during, and after the noise exposure. The cochleas were examined microscopically as plastic-embedded flat preparations. Missing cells were counted, and the sequence of degeneration was determined as a function of recovery time (0-30 days). RESULTS: With high-frequency noise, primary damage began as small focal losses of outer hair cells in the 4-8 kHz region. With continued exposure, damage progressed to involve loss of an entire segment of the organ of Corti, along with adjacent myelinated nerve fibers. Much of the latter loss is secondary to the intermixing of cochlear fluids through the damaged reticular lamina. With low-frequency noise, primary damage appeared as outer hair cell loss scattered over a broad area in the apex. With continued exposure, additional apical outer hair cells degenerated, while supporting cells, inner hair cells, and nerve fibers remained intact. Continued exposure to low-frequency noise also resulted in focal lesions in the basal cochlea that were indistinguishable from those resulting from exposure to high-frequency noise. CONCLUSIONS: The patterns of cochlear damage and their relation to functional measures of hearing in noise-exposed chinchillas are similar to those seen in noise-exposed humans. Thus, the chinchilla is an excellent model for studying noise effects, with the long-term goal of identifying ways to limit noise-induced hearing loss in humans.


Subject(s)
Cochlea/injuries , Cochlea/pathology , Disease Models, Animal , Hair Cells, Auditory/injuries , Hair Cells, Auditory/pathology , Hearing Loss, Noise-Induced/etiology , Hearing Loss, Noise-Induced/pathology , Noise/adverse effects , Animals , Auditory Threshold , Chinchilla , Disease Progression , Hearing Loss, Noise-Induced/diagnosis , Hearing Loss, Noise-Induced/prevention & control
7.
Hear Res ; 145(1-2): 111-22, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10867283

ABSTRACT

The present study was designed to determine the response to noise of the auditory system of a genetically well-defined laboratory mouse in preparation for examining the effect of noise on mice with specific genetic mutations. The mice were C57BL/CBA F1 hybrids. Eight mice served as non-noise-exposed controls and 39 mice were exposed for 1-24 h to an octave band of noise with a center frequency of 2, 4 or 8 kHz and a sound pressure level of 100-120 dB. Auditory brainstem response thresholds were measured pre-exposure and several times post-exposure (i.e., 0-27 days) to determine the magnitude of the temporary threshold shift (TTS) and permanent threshold shift (PTS). After fixation by cardiac perfusion, the cochleas from each mouse were embedded in plastic, dissected into quarter turns of the cochlear duct and analyzed quantitatively. Immediately post-exposure, all mice had sizable TTSs at the tested frequencies (i.e., 3-50 kHz). At this time, two mice were killed. Thresholds of the other 37 mice recovered somewhat in the first 4 days post-exposure. One mouse fully recovered from its TTS; 10 mice were left with PTSs at all frequencies; 26 mice recovered at some frequencies but not others. Most mice with PTSs for 30-50 kHz had focal losses of inner and outer hair cells in the basal 20% of the organ of Corti, often with degeneration of adjacent myelinated nerve fibers in the osseous spiral lamina. On the other hand, mice with PTSs for the lower frequencies (i.e., 3-20 kHz) had stereocilia disarray without significant hair cell losses in the second and first turns. Considerable variability was found in the magnitude of hair cell losses in those mice that received identical noise exposures, despite their genetic homogeneity.


Subject(s)
Cochlea/injuries , Noise , Wounds and Injuries/physiopathology , Animals , Auditory Threshold , Cilia/pathology , Cochlea/innervation , Cochlea/pathology , Cochlea/physiopathology , Evoked Potentials, Auditory, Brain Stem/physiology , Hair Cells, Auditory/pathology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Nerve Degeneration/pathology , Nerve Fibers, Myelinated/pathology , Organ of Corti/pathology , Recovery of Function , Reference Values , Time Factors , Wounds and Injuries/pathology
8.
Hear Res ; 145(1-2): 123-9, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10867284

ABSTRACT

An anatomically based frequency-place map was created for the mouse using C57BL/CBA F1 hybrids by matching noise-induced lesions in the organ of Corti with permanent hearing losses as determined by auditory brainstem response (ABR) thresholds. Twenty-six mice developed 'notched' ABR threshold shifts after exposure to an octave band of noise with a center frequency of 2 kHz at 120 dB SPL for 24 h, 4 kHz at 110 dB SPL for 4 h or 8 kHz at 100 dB SPL for 1 or 2 h. ABR thresholds were determined at several intervals post-exposure until thresholds stabilized (14-27 days). Once thresholds had stabilized, the mice were killed and their cochleas were prepared for phase-contrast microscopic examination as plastic-embedded flat preparations. Hair cell loss, stereocilia damage, and myelinated nerve fiber degeneration as a function of percentage distance from the cochlear apex were determined. Frequency-position matches could be made for 22 of the 26 mice by correlating areas of hair cell loss/stereocilia damage with permanent changes in ABR thresholds. These frequency-position data were fitted with the equation: % Distance from apex=56.6 log (f(Hz))-179.1; r(2)=0.810. This frequency-place function agrees well with Ehret's (1975) theoretical function based on critical bands and masked auditory thresholds.


Subject(s)
Cochlea/physiology , Animals , Cilia/pathology , Cochlea/innervation , Cochlea/pathology , Differential Threshold , Evoked Potentials, Auditory, Brain Stem , Hair Cells, Auditory/pathology , Hearing Loss, Noise-Induced/pathology , Hearing Loss, Noise-Induced/physiopathology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Microscopy, Phase-Contrast , Models, Biological , Nerve Degeneration/pathology , Nerve Fibers, Myelinated/pathology , Noise , Organ of Corti/pathology
9.
Hear Res ; 139(1-2): 13-30, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10601709

ABSTRACT

The structural changes associated with noise-induced temporary threshold shift (TTS) were compared to the damage associated with permanent threshold shift (PTS). A within-animal paradigm involving survival-fixation was used to minimize problems with data interpretation from interanimal variability in response to noise. Auditory brainstem response thresholds for clicks and tone pips were determined pre- and 1-2 h post-exposure in 11 chinchillas. The animals were exposed for 24 h to an octave band of noise with a center frequency of 4 kHz and a sound pressure level of 86 dB. Three animals (0/0-day) had both cochleas terminal-fixed 2-3 h post-exposure. Two animals (27/27-day) had threshold shifts determined every other day for 1 week, every week thereafter, and underwent terminal-fixation of both cochleas 27 days after exposure. Six animals (0/n-day) had threshold shifts determined in both ears upon removal from the noise; their left cochlea was then survival-fixed 2-3 h post-exposure. Threshold shifts were determined in their right ear every 2-3 days until their hearing either returned to pre-exposure values or stabilized at a reduced level at which time their right cochlea was terminal-fixed (4-13 days post-exposure). All cochleas were prepared as plastic-embedded flat preparations. Missing hair cells were counted and supporting cells and nerve fibers were evaluated throughout the organ of Corti using phase-contrast microscopy. Post-exposure, all animals had moderate TTSs in their left and right ears which averaged 43 dB for 4-12 kHz. In the 0/0-day animals, the only abnormality which correlated with TTS was a buckling of the pillar bodies. In the 0/n-day animals, their left cochlea (survival-fixed 2-3 h post-exposure) had outer hair cell (OHC) stereocilia which were not embedded in the tectorial membrane in the region of the TTS whereas OHC stereocilia were embedded in the tectorial membrane throughout the cochleas of non-noise-exposed, survival-fixed controls. Three of six right cochleas (terminal-fixed 4-13 days post-exposure) from the 0/n-day animals developed a PTS and two of these cochleas had focal losses of inner and outer hair cells and afferent nerve fibers at the corresponding frequency location. The other cochlea with PTS had buckled pillars in the corresponding frequency region. These results suggest that with moderate levels of noise exposure, buckling of the supporting cells results in an uncoupling of the OHC stereocilia from the tectorial membrane which results in a TTS. The mechanisms resulting in TTS appear to be distinct from those that produce permanent hair cell damage and a PTS.


Subject(s)
Auditory Threshold/physiology , Cochlea/injuries , Cochlea/pathology , Hearing Loss, Noise-Induced/pathology , Hearing Loss, Noise-Induced/physiopathology , Noise/adverse effects , Acoustic Stimulation , Animals , Chinchilla , Cochlea/physiopathology , Cochlear Nerve/pathology , Evoked Potentials, Auditory, Brain Stem , Hair Cells, Auditory, Inner/pathology , Hair Cells, Auditory, Outer/pathology , Microscopy, Phase-Contrast , Tectorial Membrane/pathology
10.
Hear Res ; 134(1-2): 163-78, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10452386

ABSTRACT

To minimize problems with data interpretation due to interanimal variation in susceptibility to noise, we developed a survival-fixation paradigm which involves fixing one cochlea of an experimental chinchilla at one post-exposure time and fixing the second cochlea as much as 14-24 days later. This paradigm is analytically effective because there is a high correlation in the magnitude and pattern of damage in the left and right cochleas of binaurally exposed animals. Thus, each experimental animal provides two snapshots in the degeneration and repair continua in which it can be certain that both cochleas sustained equivalent amounts of damage during the exposure. Using this technique, the time course of degeneration of different structures and cells in the organ of Corti can be determined and primary damage can be distinguished from secondary effects. The present paper discusses the issues which had to be addressed to develop this technique and provides preliminary results from chinchillas exposed to a traumatic noise.


Subject(s)
Cochlea/physiology , Tissue Fixation/methods , Animals , Cell Death/physiology , Chinchilla , Cochlea/injuries , Cochlea/pathology , Cochlea/physiopathology , Facial Nerve/physiopathology , Hair Cells, Auditory/pathology , Hair Cells, Auditory/physiology , Microscopy, Electron , Noise , Reference Values , Regeneration/physiology , Time Factors , Tissue Survival , Vestibule, Labyrinth/physiopathology
11.
Mamm Genome ; 10(6): 544-8, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10341082

ABSTRACT

The ability to sense gravity is enhanced by an extracellular structure that overlies the macular sensory epithelium. This complex consists of high density particles, otoconia, embedded within a gelatinous membrane. The tilted mouse specifically lacks otoconia, yet has no other detectable anatomic lesions. Furthermore, the penetrance of the tilted phenotype is nearly 100%. This mouse provides a model to identify genes that are involved in the development and function of vestibular otoconia. Using SSLP markers, we have mapped the tilted (tlt) gene on mouse Chromosome (Chr) 5 between D5Mit421 and D5Mit353/D5Mit128/D5Mit266/D5Mit267 by analysis of the progeny of an intersubspecific F2 intercross. We also mapped the fibroblast growth factor receptor 3 (Fgfr3) gene, a potential candidate for tlt, and the Huntington's disease homolog (Hdh) gene to D5Mit268, approximately 4.3 centiMorgans (cM) from the tilted locus. This study excludes both Fgfr3 and Hdh as candidate genes for tlt and identifies closely linked microsatellite markers that will be useful for the positional cloning of tlt.


Subject(s)
Mice, Mutant Strains/genetics , Otolithic Membrane/abnormalities , Physical Chromosome Mapping/methods , Protein-Tyrosine Kinases , Animals , Centromere , Crosses, Genetic , Genetic Markers , Huntingtin Protein , Meiosis , Mice , Mice, Inbred Strains , Nerve Tissue Proteins/genetics , Nuclear Proteins/genetics , Polymerase Chain Reaction , Receptor, Fibroblast Growth Factor, Type 3 , Receptors, Fibroblast Growth Factor/genetics , Swimming/physiology
13.
Hear Res ; 124(1-2): 132-45, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9822911

ABSTRACT

Eleven chinchillas between 1 and 2.4 years of age had the malleus/incus complex removed from one middle ear and then lived in the Washington University animal facilities for 4 years post-surgery. Each animal had one ear (termed ambient-noise) in which the conductive apparatus was intact; the other ear (termed noise-protected) had a 50-60 dB conductive hearing loss. The background sound level in the animal facility was 59 dBA with periodic brief sounds up to 102 dBA. After the 4-year experimental period, both ears were fixed, embedded in plastic and dissected for microscopic examination as flat preparations. The quantitative and qualitative findings in the noise-protected ears were compared to those in the ambient-noise ears. Both groups of ears sustained losses of sensory and supporting cells throughout the organ of Corti. A variable amount of age pigment was found to have accumulated in the outer hair cells and all supporting cells. In the noise-protected ears, inner hair cell loss ranged from 1.0 to 3.1% and averaged 1.7 +/- 0.8%; outer hair cell loss ranged from 1.8 to 6.4% and averaged 3.6 +/- 1.2%. In the ambient-noise ears, inner hair cell loss ranged from 0.7 to 2.8% and averaged 1.6 +/- 0.7%; outer hair cell loss ranged from 1.3 to 5.4% and averaged 3.6 +/- 1.2%. Within-animal comparison of cell losses in the noise-protected and ambient-noise ears revealed no significant difference between the two groups. It is concluded that long-term exposure to micro-noise does not accelerate the spontaneous loss of sensory cells which occurs with aging. Although not quantified, there was no obvious difference in the amount or cellular distribution of age pigment in the two groups. Thus, it appears that the formation of age pigment in the ear is the result of the cells' basic metabolic processes rather than the wear and tear from sensory transduction.


Subject(s)
Aging/physiology , Ear, Inner/injuries , Ear, Inner/physiopathology , Noise , Animals , Blood Vessels/pathology , Cell Count , Chinchilla , Ear Ossicles/physiology , Ear, Inner/blood supply , Ear, Inner/pathology , Hair Cells, Auditory/pathology , Hearing Loss, Conductive/etiology , Hearing Loss, Conductive/pathology , Hearing Loss, Conductive/physiopathology , Nerve Degeneration/pathology , Nerve Fibers, Myelinated/pathology , Otologic Surgical Procedures , Stria Vascularis/pathology
14.
Hear Res ; 122(1-2): 60-70, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9714575

ABSTRACT

The sense of balance is one of the phylogenetically oldest sensory systems. The vestibular organs, consisting of sensory hair cells and an overlying extracellular membrane, have been conserved throughout vertebrate evolution. To better understand mechanisms regulating vestibular development and mechanisms of vestibular pathophysiology, we have analyzed the mouse mutant, tilted (tlt), which has dysfunction of the gravity receptors. The tilted mouse arose spontaneously and has not been previously analyzed for a developmental or physiological deficit. Here we demonstrate that the tilted mouse, like the head tilt (het) mouse, specifically lacks otoconia and consequently does not sense spatial orientation relative to the force of gravity. Unlike other mouse mutations affecting the vestibular system (such as pallid, mocha and tilted head), the defect in the tilted mouse is highly penetrant, results in the nearly complete absence of otoconia, exhibits no degeneration of the sensory epithelium and has no apparent abnormal phenotype in other organ systems. We further demonstrate that protein expression in the macular sensory epithelium is qualitatively unaltered in tilted mutant mice.


Subject(s)
Auditory Threshold/physiology , Glycoproteins/biosynthesis , Head-Down Tilt , Mice, Mutant Strains/abnormalities , Otolithic Membrane/abnormalities , Animals , Epithelium/physiopathology , Evoked Potentials, Auditory, Brain Stem/genetics , Female , Glycoproteins/analysis , Male , Mice , Mice, Inbred C57BL , Mice, Mutant Strains/genetics , Microscopy, Electron , Mutation/physiology , Organ of Corti/physiopathology , Otolithic Membrane/physiopathology , Otolithic Membrane/ultrastructure , Phenotype , Postural Balance/physiology
15.
Microsc Res Tech ; 41(3): 205-16, 1998 May 01.
Article in English | MEDLINE | ID: mdl-9605338

ABSTRACT

Adult chinchillas were exposed once to an octave-band noise, centered at 4 kHz, and allowed to survive for 16 days or for 1, 2, 4, and 8 months. Axonal degeneration was mapped in the cochlear nucleus, using the Nauta-Rasmussen silver method, and related to hair cell damage and to loss of myelinated nerve fibers in the osseous spiral lamina of the cochlea. Axonal degeneration in the dorsal cochlear nucleus had already reached a peak by 16 days and disappeared after 1 month. Meanwhile, myelinated nerve fiber degeneration in the cochlea extended basally, followed 2 weeks to 2 months later by spread of axonal degeneration into the corresponding high-frequency region of the ventral cochlear nucleus. Axonal degeneration occurred early in the low-frequency region of the ventral cochlear nucleus, followed 2-4 weeks later by spread of myelinated fiber degeneration into more apical regions of the cochlea. New degeneration of axons in the cochlear nerve and in the ventral cochlear nucleus continued to occur for up to 8 months after stimulation. These findings imply that plastic changes in the central auditory pathways could play a role in the long-term effects of cochlear damage and acoustic overstimulation, possibly leading to a chronic neurodegenerative condition in the ear and in the brain.


Subject(s)
Acoustic Stimulation/adverse effects , Cochlear Nerve/pathology , Cochlear Nucleus/pathology , Nerve Degeneration/pathology , Animals , Chinchilla , Hair Cells, Auditory/pathology , Nerve Degeneration/etiology , Silver Staining/methods , Time Factors
16.
Exp Neurol ; 147(2): 256-68, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9344551

ABSTRACT

This study determined the effect of acoustic overstimulation of the adult cochlea on axons in the cochlear nucleus. Chinchillas were exposed to an octave-band noise centered at 4 kHz at 108 dB sound pressure level for 1.75 h. One chinchilla was never exposed to the noise, and several others had one ear protected by an ear plug or prior removal of the malleus and incus. Exposure of unprotected ears caused loss of inner and outer hair cells and myelinated nerve fibers, mostly in the basal half of the cochlea. Cochlear nerve fiber degeneration, ipsilateral to the exposed ears, was traced to regions of the cochlear nucleus representing the damaged parts of the cochlea. In silver impregnations of a deafferented zone in the posteroventral cochlear nucleus, the concentration of axons decreased by 43% after 1 month and by 54% after 2 months. However, by 8 months, the concentration of thinner axons, with diameters of less than 0.46 microm, increased by 46-90% over that at 2 months. The concentration of axons with larger diameters did not change. Between 2 and 8 months small axonal endings appeared next to neuronal cell bodies. This later increase of thinner axons and endings is consistent with a reactive growth of new axons of relatively small diameter. The emergence of small perisomatic boutons suggests that the new axons formed synaptic endings, which might contribute to an abnormal reorganization of the central auditory system and to the pathological changes that accompany acoustic overstimulation.


Subject(s)
Axons/ultrastructure , Cochlear Nucleus/pathology , Hearing Loss, Noise-Induced/pathology , Nerve Regeneration , Animals , Atrophy , Auditory Pathways/pathology , Cell Count , Chinchilla , Denervation , Ear Protective Devices , Hair Cells, Auditory/pathology , Hearing Loss/etiology , Hearing Loss/pathology , Nerve Degeneration , Nerve Fibers/pathology , Organ of Corti/pathology , Synapses/pathology
17.
Hear Res ; 109(1-2): 34-45, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9259234

ABSTRACT

A technique has been developed for preparing the mouse temporal bone for histopathological examination: first, as a whole mount to detect any gross malformations of the bony or membranous labyrinths; second, in dissected segments to localize damage in the different sensory organs and to quantify sensory- and supporting-cell losses; and finally, in semi-thick and thin sections to identify and characterize subcellular pathology. Examples are given of the successful application of this technique to mice with very different inner-ear problems, including those with an abnormally short cochlear spiral, a defective lateral semicircular canal, abnormal otoliths over the saccular macula, an increased susceptibility to noise damage and those which lack fibroblast growth factor receptor 3.


Subject(s)
Cochlea/pathology , Temporal Bone/pathology , Animals , Cochlea/ultrastructure , Hair Cells, Auditory, Inner/pathology , Hair Cells, Auditory, Inner/ultrastructure , Hair Cells, Auditory, Outer/pathology , Hair Cells, Auditory, Outer/ultrastructure , Mice , Microscopy, Electron , Microscopy, Phase-Contrast , Mutation/genetics , Nerve Fibers/ultrastructure , Plastic Embedding , Saccule and Utricle/pathology , Spiral Ganglion/cytology , Spiral Ganglion/pathology , Temporal Bone/ultrastructure , Tissue Fixation
18.
Int J Dev Neurosci ; 15(4-5): 601-17, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9263037

ABSTRACT

The time course of events which are essential for nerve-fiber regeneration in the mammalian cochlea was determined using a group of chinchillas that had been exposed for 3.5 hr to an octave band of noise with a center frequency of 4 kHz and a sound pressure level of 108 dB. The animals recovered from 40 min (0 days) to 100 days at which times their inner ears were fixed and the organs of Corti prepared for phase-contrast and bright-field microscopy as plastic-embedded flat preparations. Selected areas identified in the flat preparations were semi-thick and thin sectioned at radial or tangential angles for examination by bright-field and transmission electron microscopy. The following time-ordered events appeared critical for nerve-fiber regeneration: (1) The area of the basilar membrane in which regeneration had a possibility of occurring showed signs of severe injury. Outer hair cells degenerated first followed by outer pillars, inner pillars, inner hair cells and other supporting cells; (2) Myelinated nerve fibers in the osseous spiral lamina became fragmented, starting at the distal ends of the fibers. This degeneration gradually extended back to Rosenthal's canal; (3) Fibrous processes, originating from Schwann-like cells in the osseous spiral lamina, extended laterally on the basilar membrane; (4) Schwann cells lined up medial to the habenulae perforata in the areas of severest damage, apparently ready to migrate through the habenulae onto the basilar membrane; (5) Schwann-cell nuclei appeared on the basilar membrane beneath the developing layer of squamous epithelium which was in the process of replacing the degenerated portion of the organ of Corti; (6) Regenerated nerve fibers with thin myelin sheaths or a simple investment of Schwann cell cytoplasm appeared in areas of total loss of the organ of Corti; and (7) The myelin sheaths on the regenerated nerve fibers gradually became thicker.


Subject(s)
Cochlea/pathology , Hearing Loss, Noise-Induced/pathology , Nerve Fibers/physiology , Nerve Regeneration/physiology , Animals , Chinchilla , Epithelium/pathology , Epithelium/ultrastructure , Membranes/pathology , Membranes/ultrastructure , Microscopy, Electron , Nerve Degeneration/physiology , Neurons/pathology , Neurons/ultrastructure , Organ of Corti/pathology , Rabbits , Schwann Cells/physiology , Schwann Cells/ultrastructure , Time Factors
19.
Hear Res ; 103(1-2): 151-68, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9007582

ABSTRACT

Terminal axonal degeneration in the brain following cochlear lesions was studied with the Nauta-Rasmussen method. Losses of hair cells and myelinated cochlear fibers were assessed. The cochleotopic map projected, from apex to base, on the ventral-to-dorsal axes of the cochlear nuclei. The cochleotopic correspondence was better for loss of cochlear nerve fibers and inner hair cells, than for outer hair cells. Cochlear fibers were traced to all parts of the cochlear nucleus, including the small-cell shell, also to cell-group Y and the flocculus. Terminal axonal degeneration in nuclei of the superior olivary complex, lateral lemniscus, and inferior colliculus was interpreted as transynaptic, since degenerated axons could not be traced to these locations from the cochlear nerve or trapezoid body. Moreover, biotinylated dextran amine injection in the basal turn of scala media of a normal cochlea labeled cochlear nerve fibers projecting to the high-frequency regions of the cochlear nuclei and to the flocculus, but not to more central auditory nuclei. This is the first detailed account of transynaptic degeneration in the ascending auditory pathway resulting from cochlear damage in an adult mammal. These findings are consistent with a dystrophic process depending on hair-cell loss and/or direct damage to cochlear nerve fibers.


Subject(s)
Chinchilla/anatomy & histology , Cochlea/injuries , Cochlea/innervation , Animals , Auditory Pathways/pathology , Auditory Pathways/physiopathology , Axons/pathology , Cerebellum/pathology , Chinchilla/physiology , Cochlea/pathology , Cochlear Nerve/pathology , Cochlear Nucleus/pathology , Hair Cells, Auditory/pathology , Nerve Degeneration , Nerve Fibers, Myelinated/pathology , Olivary Nucleus/pathology
20.
Hear Res ; 103(1-2): 169-91, 1997 Jan.
Article in English | MEDLINE | ID: mdl-9007583

ABSTRACT

The patterns of axonal degeneration following acoustic overstimulation of the cochlea were traced in the brainstem of adult chinchillas. The Nauta-Rasmussen method for axonal degeneration was used following survivals of 1-32 days after a 105 min exposure to an octave-band noise with a center frequency of 4 kHz and a sound pressure level of 108 dB. Hair-cell and myelinated nerve-fiber loss were assessed in the cochlea. The cochleotopic pattern of terminal degeneration in the ventral cochlear nucleus correlated with the sites of myelinated fiber and inner-hair-cell loss: this correlation was less rigorous with outer-hair-cell loss, especially in the dorsal cochlear nucleus. These results are consistent with a dystrophic process with a slow time course depending on hair-cell loss and/or direct cochlear nerve-fiber damage. However, in a number of cases with no damage in the apical cochlea, fine fiber degeneration occurred with a faster course in low-frequency regions in the dorsal cochlear nucleus and, transynaptically, in a non-cochleotopic pattern in the superior olive and inferior colliculus. These findings suggest that neuronal hyperactivity plays a role in the central degeneration following acoustic overstimulation, possibly by an excitotoxic process.


Subject(s)
Axons/pathology , Brain Stem/pathology , Chinchilla/anatomy & histology , Cochlea/injuries , Cochlea/pathology , Noise/adverse effects , Acoustic Stimulation/adverse effects , Animals , Auditory Pathways/injuries , Auditory Pathways/pathology , Brain Stem/injuries , Chinchilla/physiology , Cochlear Nucleus/pathology , Hair Cells, Auditory, Inner/pathology , Hair Cells, Auditory, Outer/pathology , Inferior Colliculi/pathology , Nerve Degeneration , Nerve Fibers, Myelinated/pathology , Olivary Nucleus/pathology
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