ABSTRACT
Studies were performed on epithelial cells obtained from uterine cavity in 49 women. All cases were subdivided into four groups; unaltered endometrium-9, glandular endometrial hyperplasia-10, atypical hyperplasia-4 and endometrial adenocarcinoma-30. For fluorimetric evaluation of single- and double-strand DNA specimens were dyed with acridine orange, which is known to produce red fluorescence in combination with double-strand acids. Values of coefficients alpha for single and double-strand DNA after acid hydrolysis were much higher than those before acid treatment. This study provides a deeper insight into the relation between DNA lesions and malignancy.
Subject(s)
Adenocarcinoma/ultrastructure , DNA, Neoplasm/ultrastructure , DNA, Single-Stranded/ultrastructure , DNA/ultrastructure , Endometrial Hyperplasia/pathology , Endometrial Neoplasms/ultrastructure , Cell Transformation, Neoplastic/ultrastructure , Drug Stability , Endometrium/ultrastructure , Epithelium/ultrastructure , Female , Flow Cytometry/instrumentation , Humans , Microscopy, Fluorescence/instrumentationABSTRACT
A clinico-genealogic investigation was carried out in 216 patients with endometrial cancer. Familial accumulation of endometrial and other cancer incidence was established. The segregation rates appeared to be lower than those expected from simple Mendelian models (2-11%). A multifactorial nature of endometrial cancer in overall susceptibility to the disease was found to be at 61%. A genetic correlation analysis showed endometrial cancer to share common genes with breast and gastric cancer in females. Tables of recurrent risk of the disease for relatives were prepared to be used in medico-genetic counseling.
Subject(s)
Endometrial Neoplasms/genetics , Genetics, Population , Adult , Endometrial Neoplasms/epidemiology , Female , Genotype , Humans , Incidence , Male , Middle Aged , Neoplasms/epidemiology , Neoplasms/genetics , Phenotype , Risk Factors , Ukraine/epidemiologyABSTRACT
Literature on application of DNA acid hydrolysis curves to cytochemical studies of tumours and invariable homologous tissues is reviewed. It is shown that multiapex DNA acid hydrolysis curves typical of tumours reflect physical and chemical characteristics of DNP malignant cells.