Inflammatory bowel disease and depressive symptoms: the prevalence and factors associated with depression in patients with inflammatory bowel disease on intravenous biological therapy - single center experience.

OBJECTIVE: Depression is one of the most commonly occurring psychiatric comorbidities in patients suffering from inflammatory bowel disease (IBD). This study aims to determine prevalence and risk factors for the more severe symptoms of depression (DP) in IBD patients on intravenous biological therapy (IBT). PATIENTS AND METHODS: The study consisted of 90 IBD patients who completed a Patient Health Questionnaire-9 (PHQ9) to detect symptoms of depression. Demographic information and disease characteristics were collected as well as medication information for these patients. Univariate and multivariate ordinal logistic regression was done to identify risk factors for the DP. RESULTS: Anti-TNF therapy comprised 58.9% of patients and anti-integrin 41.1%. The prevalence of DP (PHQ9score ≥10) among these patients is 20%. For the univariate logistic regression DP was statistically significantly associated with disease activity (OR 6.656; 95% Cl 2.576-17.19, p<0.001), use of corticosteroids (OR 4.224; 95% Cl 1.658-10.76, p = 0.003) and thiopurine (OR 2.502 95% Cl 1.031-6.069, p = 0.042), as well as relationship status (single, in relationship or married) (OR 0.391; 95% Cl 0.173-0.885, p = 0.024). The multivariate analysis indicated that the risk of developing DP was associated with disease activity (OR 5.708; 95% Cl 2.138-15.23, p = 0.001). CONCLUSIONS: Our study shows that most of severe symptoms of depression were present in 20% of the IBD patients examined who were receiving intravenous biological therapy. Particular attention and efforts should, therefore, be focused on patients who have an active form of the disease.

Variability of essential oils of Satureja montana L. and Satureja kitaibelii wierzb. ex Heuff. from the central part of the balkan peninsula.

The results of the essential oil composition confirm that there are differences between populations within the same subspecies (Satureja montana ssp. montana, between the subspecies (Satureja montana ssp. montana and Satureja montana ssp. pisidica) and the species (Satureja montana and S. kitabelii). The essential oil composition of S. montana ssp. montana is more related to the Satureja montana ssp. pisidica than S. kitaibelii which separates as a whole species.

Bacterial penetration of restored cavities.

The aim of this study was to assess the quality of the marginal seals of 7 restoratives by means of a bacterial penetration test in vitro. Sixty intact premolars and third molars that were scheduled for extraction were used in the test. There were 2 experimental groups of teeth, as follows: (1) A class V conventional cavity and a wedge erosion cavity were prepared on the buccal surface and the lingual surface, respectively, of each tooth. (2) A class V conventional cavity and a wedge erosion cavity were prepared on the buccal surface and the lingual surface, respectively, of each tooth with a completely removed enamel layer. The cavities were then reconstructed with different restorative materials. The quality of the marginal seals was evaluated by submerging the teeth in a bacterial suspension and incubating them in an anaerobic milieu at 37 degrees C for 20 hours. The teeth were subsequently processed for histologic data and bacterial staining. The best marginal sealing in both the wedge erosion and the class V cavities was provided by the Herculite/Optibond system and the Valux Plus/Scotchbond Multipurpose system. Bacterial penetration was slightly greater with the Luxat compomer and the Dyrect compomer, as well as with Vitremer glass ionomer cement and Fuji LC glass ionomer cement. The bacterial penetration test showed that the use of restorative material does not entirely eliminate microleakage.

[Histologic manifestations in the skin in alpha-lactoalbumin intradermal tests in patients with chronic urticaria]. / Histoloske pojave u kozi u toku intradermnog testa alfa-laktoalbuminom kod bolesnika s hronicnom urtikarijom.

Intradermal injection with alpha-lactoalbumine, 2000 PNU/ml was carried out in seven patients (1 male, 6 females, mean age 32.4 years) suffering from milk intolerance. None of the patients had a positive allergen-specific IgE antibodies in the sera (RAST). Skin test was performed on the volar part of the forearms at the same time. The area of skin reaction was estimated and measured within 36 hours on the right arm. Skin biopsy was done with a 4 mm punch after 8 hours from site of antigen injection on the left arm. Various cells infiltrating the site of antigen instillation were present. Leukocytoclastic vasculitis was only found in patients with dual intradermal reaction (immediate and late onset of response; 8 12 hours). These results indicate that local skin reaction to alpha-lactoal-bumine is mediated by diverse immune mechanisms, and that it may be of clinical significance for the diagnosis of cow milk allergy in adults.

[The oral milk provocation test in patients with chronic urticaria]. / Oralni provokacioni test mlekom u bolesnika sa hronicnom urtikarijom.

Histamine and sera complement were followed-up in patients with chronic urticaria during oral provoking test to cow milk. Three patients with a positive history to cow milk sensitisation showed a significant decrease in C3 concentration within 180 minutes of the test. This was followed by increased histamine and skin eruption. According to our results (skin test reactivity and clinical evaluation of the provoking test) several mechanisms were involved in the sensitisation to cow milk in our patients.

[Spontaneous recanalization of tubes after surgical sterilization]. / Spontana rekanalizacija tuba posle operativne sterilizacje.

The authors present their 13 cases of sterilization in which a spontaneous recanalization of tubes occurred. Out of these 13 cases, 11 underwent sterilization after the operative Madlener method, while in the remaining 2 cases a laparoscopic method by thermocoagulation was applied. Recanalization in most cases (7) developed 15 or more months after operative sterilization. An adequate passage of tubes was verified in 12 cases through pregnancy, while only in one case HSG was applied, showing a spontaneous recanalization of the right tube.