ABSTRACT
Experiments were performed on cultured slices of rat ventral hippocampus. Using extracellular stimulation and patch clamp recording from pyramidal neurons in the hippocampal CA1 area, we studied characteristics of GABAergic synapse formed on these neurons by cholecystokinin-expressing interneurons. This synapse was characterized by asynchronous release of GABA and depolarization-induced suppression of inhibitory response. It was observed that administration of corticosterone increased the amplitude of evoked inhibitory postsynaptic currents in 5 minutes, but the paired ratio did not significantly change. Obtained data reflect that corticosterone can induce rapid genome-independent effects on inhibitory neurotransmission in one of hippocampal synapses.
Subject(s)
Action Potentials/drug effects , Corticosterone/pharmacology , Evoked Potentials/drug effects , GABAergic Neurons/metabolism , Hippocampus/drug effects , Synaptic Transmission/drug effects , gamma-Aminobutyric Acid/metabolism , Animals , Cholecystokinin/biosynthesis , Electric Stimulation , Hippocampus/physiology , Interneurons/metabolism , Patch-Clamp Techniques , Pyramidal Cells/physiology , Rats , Rats, Wistar , Synapses/drug effectsABSTRACT
Transfection is a method of transforming cells based on the introduction into living cells of plasmids encoding a particular protein or RNA. This review describes the main methods of transfection and considers their advantages and disadvantages. Most attention is paid to lentivirus transduction as one of the most efficient methods for transforming nerve cells. The development of current transfection systems based on lentivirus vectors is described and a brief review of studies performed using in vivo and in vitro lentivirus transfection of nerve cells is presented.
Subject(s)
Genetic Vectors , Lentivirus , Neurons/metabolism , Transfection/methods , Animals , Cells, Cultured , Humans , Plasmids/metabolism , RNA, Small Interfering/metabolismABSTRACT
A review. Transfection is a method of cell transformation based on the introduction of a plasmid encoding a protein or RNA into the living cells. Basic methods of transfection and their strengths and weaknesses are discussed. A large part of the review is focused on the lentiviral transduction as one of the most efficient methods of transformation of nerve cells. The development of modern systems of lentiviral transfection is traced and a number of works performed with the use of in vivo and in vitro lentiviral transfection of the nervous system are reviewed.
