Subject(s)
Adrenal Cortex Hormones/pharmacology , Thymus Gland/drug effects , Adrenalectomy , Animals , Cell Separation , Cholesterol/biosynthesis , DNA-Directed RNA Polymerases/metabolism , Depression, Chemical , Drug Resistance , Glucocorticoids/pharmacology , Glucose/metabolism , Kinetics , Lipids/biosynthesis , Mice , RNA/biosynthesis , Thymus Gland/cytology , Thymus Gland/metabolismSubject(s)
Glucocorticoids/metabolism , Receptors, Glucocorticoid/metabolism , Receptors, Steroid/metabolism , Thymus Gland/metabolism , Animals , Binding Sites , Cell Membrane/metabolism , Cell Nucleus/metabolism , Chemical Phenomena , Chemistry, Physical , Cytoplasm/metabolism , Dose-Response Relationship, Drug , Drug Interactions , In Vitro Techniques , Kinetics , Phosphorylation , Rats , TemperatureSubject(s)
Enzyme Inhibitors/pharmacology , Inactivation, Metabolic , Animals , Binding Sites/drug effects , Dose-Response Relationship, Drug , Endoplasmic Reticulum/enzymology , Enzyme Activation/drug effects , Enzyme Induction/drug effects , Enzyme Inhibitors/metabolism , Hydroxylation , In Vitro Techniques , Kinetics , Metabolic Diseases/enzymology , Microsomes/enzymology , Microsomes, Liver/enzymology , Oxidation-Reduction/drug effects , Time FactorsABSTRACT
Uro- and cholecystographic contrast media are shown to be capable of changing the rate of oxidative hydroxylation of amidopyrine and steroid hormones. The degree and duration of the diminishing rate of the xenobiotics and steroids metabolism are determined by concentrations of the X-ray contrast media in hepatic cells and the rate of their clearance.
Subject(s)
Aminopyrine/metabolism , Contrast Media/pharmacology , Estradiol/metabolism , Microsomes, Liver/drug effects , Testosterone/metabolism , Animals , Depression, Chemical , Hydroxylation , Iodine , Male , Oxidation-Reduction , RatsABSTRACT
The effect of roentgen-contrast media on the activity of the NADP'N and NAD'N-dependent electron-transport chains of the rats liver microsomes was studied. Bilignost, cardiotrast, triiotrast and triombrin are shown to lower the rate of the NADP'N oxidation by the rats' liver microsomes and have no effect (except for triiotrast) upon the rate of the NAD'N oxidation. It is presumed that the relative resistance of the NAD'N-specific flavoproteid is due to the presence of a hydrophobic layer impervious to the polar molecules of the contrast media.
Subject(s)
Contrast Media/pharmacology , Microsomes, Liver/metabolism , NADP/metabolism , NAD/metabolism , Acetrizoic Acid/pharmacology , Animals , Diatrizoate/pharmacology , Iodipamide/pharmacology , Iodopyracet/pharmacology , Male , Microsomes, Liver/drug effects , Oxidation-Reduction , RatsABSTRACT
A study was made of the immunodepressive effect of cyclophosphamide (CP) on mice of 3 strains (BALB/c, CBA, and DBA/2) immunized with sheep red blood cells (SRBC). With the optimal immunizing dose of the antigen (5 X 10(8) SRBC) the most pronounced immunodepression was noted in DBA/2 mice, and with the high dose (6.2 X 10(9))--in DBA/2 and CBA mice. The CP action proved to depend on the dose of the antigen administered; in BALB/c mice a reduction in the number of the antibody-forming cells was the same with both SRBC doses, in DBA/2 mice an increase of the antigen dose led to reduction of immunode pression, and in CBA mice -- to its enhancement (with sufficiently high CP doses). Determination of the rate of oxidative CP hydroxylation by the liver microsomes of mice showed it to be comparatively low in DBA/2 and CBA mice, and much greater in BALB/c mice. It is supposed that the detected differences in the immunodepressive action of CP could be connected with different sensitivity of the target cells and (or) with the peculiarities of its metabolism in mice belonging to different strains.
Subject(s)
Antibody-Producing Cells/drug effects , Cyclophosphamide/pharmacology , Immunosuppressive Agents/pharmacology , Animals , Biotransformation , Cyclophosphamide/metabolism , Liver/metabolism , Male , Mice , Mice, Inbred BALB C , Mice, Inbred CBA , Mice, Inbred DBA , Species Specificity , Spleen/drug effects , Time FactorsSubject(s)
Cytochrome Reductases/metabolism , Endoplasmic Reticulum/enzymology , NADPH-Ferrihemoprotein Reductase/metabolism , Pharmaceutical Preparations/metabolism , Radiation Effects , Aminopyrine/metabolism , Animals , Biotransformation , Biphenyl Compounds/metabolism , Cytochrome P-450 Enzyme System/analysis , Endoplasmic Reticulum/radiation effects , Hexobarbital/metabolism , Hormones/metabolism , Hydroxylation , Liver/cytology , Male , Meperidine/metabolism , Microsomes/analysis , Microsomes/radiation effects , Models, Biological , NADP/metabolism , Oxygen Consumption , Radiation Dosage , Radiation, Ionizing , Rats , Time FactorsABSTRACT
gamma-irradiation in doses of over 600 r proved to decrease the rate of androgen hydroxylation significantly; the rate of hydroxylation of the estrogens under study altered but little. Under the mentioned irradiation conditions the content of cytochrome P-450 in rat hepatic microsomes diminished. A reduction of the hydroxylation rate of steroid hormones by rat hepatic microsomes under the effect of total gamma-irradiation apparently depended on the qualitative changes of cytochrome P-450.
