Comparison of Measurements of External Load between Professional Soccer Players.

Background: The excessive and rapid increases in training load (TL) may be responsible for most non-contact injuries in soccer. This study's aims were to describe, week(w)-by-week, the acute (AW), chronic (CW), acute:chronic workload ratio (wACWR), total distance (wTD), duration training (wDT), sprint total distance (wSTD), repeat sprint (wRS), and maximum speed (wMS) between starter and non-starter professional soccer players based on different periods (i.e., pre-, early-, mid-, and end-season) of a full-season (Persian Gulf Pro League, 2019−2020). Methods: Nineteen players were divided according to their starting status: starters (n = 10) or non-starters (n = 9). External workload was monitored for 43 weeks: pre- from w1−w4; early- from w5−w17; mid- from w18−w30, and end-season from w31−w43. Results: In starters, AW, CW, and wACWR were greater than non-starters (p < 0.05) throughout the periods of early- (CW, p ≤ 0.0001), mid- (AW, p = 0.008; CW, p ≤ 0.0001; wACWR, p = 0.043), or end-season (AW, p = 0.035; CW, p = 0.017; wACWR, p = 0.010). Starters had a greater wTD (p ≤ 0.0001), wSTD (p ≤ 0.0001 to 0.003), wDT (p ≤ 0.0001 to 0.023), wRS (p ≤ 0.0001 to 0.018), and wMS (p ≤ 0.0001) than non-starters during early-, mid-, and end-season. Conclusion: Starters experienced more CW and AW during the season than non-starters, which underlines the need to design tailored training programs accounting for the differences between playing status.

Endurance Training Regulates Expression of Some Angiogenesis-Related Genes in Cardiac Tissue of Experimentally Induced Diabetic Rats.

Exercise can ameliorate cardiovascular dysfunctions in the diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of the present study was to determine the impact of endurance training on expression of angiogenesis-related genes in cardiac tissue of diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N = 10) including diabetic training (DT), sedentary diabetes (SD), and sedentary healthy (SH), in which diabetes was induced by a single dose of streptozotocin (50 mg/kg). Endurance training (ET) with moderate-intensity was performed on a motorized treadmill for six weeks. Training duration and treadmill speed were increased during five weeks, but they were kept constant at the final week, and slope was zero at all stages. Real-time polymerase chain reaction (RT-PCR) analysis was used to measure the expression of myocyte enhancer factor-2C (MEF2C), histone deacetylase-4 (HDAC4) and Calmodulin-dependent protein kinase II (CaMKII) in cardiac tissues of the rats. Our results demonstrated that six weeks of ET increased gene expression of MEF2C significantly (p < 0.05), and caused a significant reduction in HDAC4 and CaMKII gene expression in the DT rats compared to the SD rats (p < 0.05). We concluded that moderate-intensity ET could play a critical role in ameliorating cardiovascular dysfunction in a diabetes condition by regulating the expression of some angiogenesis-related genes in cardiac tissues.

Role of melatonin in the angiogenesis potential; highlights on the cardiovascular disease.

Melatonin possesses multi-organ and pleiotropic effects with potency to control angiogenesis at both molecular and cellular levels. To date, many efforts have been made to control and regulate the dynamic of angiogenesis modulators in a different milieu. The term angiogenesis or neovascularization refers to the development of de novo vascular buds from the pre-existing blood vessels. This phenomenon is tightly dependent on the balance between the pro- and anti-angiogenesis factors which alters the functional behavior of vascular cells. The promotion of angiogenesis is thought to be an effective strategy to accelerate the healing process of ischemic changes such as infarcted myocardium. Of note, most of the previous studies have focused on the anti-angiogenesis capacity of melatonin in the tumor niche. To the best of our knowledge, few experiments highlighted the melatonin angiogenesis potential and specific regulatory mechanisms in the cardiovascular system. Here, we aimed to summarize some previous experiments related to the application of melatonin in cardiovascular diseases such as ischemic injury and hypertension by focusing on the regulatory mechanisms.

Melatonin and prolonged physical activity attenuated the detrimental effects of diabetic condition on murine cardiac tissue.

In this study, the combined effects of four-week swimming training and melatonin were examined on the oxidative response, inflammation, apoptosis, and angiogenesis capacity of cardiac tissue in the mouse model of diabetes. The mice were randomly allocated into five groups (n = 10 per group) as follows: Control; Diabetic group; Diabetic + Melatonin group; Diabetic + Exercise group; and Diabetic + Exercise + Melatonin group. 50 mg/kg streptozotocin was intraperitoneally administrated. In melatonin-treated groups, melatonin was injected intraperitoneally at 3 mg/kg body weight for four weeks and twice weekly. Swimming exercises were performed for four weeks. We measured cardiac superoxide dismutase, glutathione peroxidase enzymes, malondialdehyde, and total antioxidant capacity. The expression of tumor necrosis factor-α, Caspase­3, Sirtuin1, and Connexin-43 was measured using real-time PCR analysis. The vascular density was analyzed by immunohistochemistry using CD31 and α-smooth muscle actin antibodies. The combination of melatonin and exercise elevated cardiac superoxide dismutase, glutathione peroxidase coincided with the reduction of malondialdehyde and increase of total antioxidant capacity as compared to the diabetic mice (p < 0.05). In Diabetic + Exercise + Melatonin mice, tumor necrosis factor-α, Caspase­3 was significantly down-regulated compared to the Diabetic group (p < 0.05). Melatonin and exercise suppressed the expression of Connexin-43 and Sirtuin1 in diabetic mice in comparison with the control mice (p < 0.05). H & E staining showed necrosis and focal hyperemia reduction in the Diabetic + Exercise + Melatonin group compared to the Diabetic group. Data showed a decrease of CD31+ and α-smooth muscle actin+ vessels in the Diabetic group as compared to the normal samples (p < 0.05). The number of CD31+ vessels, but not α-smooth muscle actin+ type, increased in the Diabetic + Exercise + Melatonin group compared to the Diabetic mice. These data demonstrated that exercise along with melatonin administration could diminish the detrimental effects of diabetes on cardiac tissue via using different mechanisms.

Is the cardiorespiratory fitness affected by height of young girls?

The purpose of the present study was to examine the effect of height in the predicted VO2max by the Queens Step test among short and tall young girls. A sample of 38 individuals was selected in two stages from a total of 500 individuals and was assigned to two groups of short (n = 20) and tall (n = 18). In order to examine the effect of height in the predicted VO2max, the Queens step protocol and the incremental treadmill speed test were used. Respiratory exchange was measured continuously throughout the test by an automated open-circuit gas analysis system. The study results showed that tall girls revealed a higher VO2max on the Queen's step and treadmill tests than short girls (Queen's: 44.09+/-2.66 vs. 38.96+/-1.65; Treadmill: 34.03+/-7.26 vs. 28.15+/-5.09 mL/kg/min). Based on the obtained findings it can be concluded that the higher VO2max seen in tall girls on the both protocols, may be due to their physiological and physical properties; therefore, it seems that designing of the adjustable steps to the height of subjects for optimizing the estimation of VO2max is not necessary and other physiological factors may be involved, which require further investigation.