1.
Transplant Proc
; 24(1): 208-10, 1992 Feb.
Article
in English
| MEDLINE
| ID: mdl-1531709
Subject(s)
Antigen-Presenting Cells/immunology , Antigens, CD/analysis , T-Lymphocytes/immunology , Transplantation, Heterologous/immunology , Animals , Antibodies, Monoclonal , Antigens, Differentiation, T-Lymphocyte/analysis , CD3 Complex , Humans , Lymphocyte Activation , Lymphocyte Culture Test, Mixed , Receptors, Antigen, T-Cell/analysis
2.
Gene
; 84(1): 73-81, 1989 Dec 07.
Article
in English
| MEDLINE
| ID: mdl-2481606
ABSTRACT
Various regions of the gene encoding the major neutralization antigen, VP7, of human rotavirus have been expressed in Escherichia coli, as N-terminal fusions to beta-galactosidase under the control of the lac promoter. We have determined that the fusion products of two clones containing regions AB (aa 69-158) and ABC (aa 69-319) were antigenic, reacting with antibodies raised against whole virus. When guinea pigs were immunized with fusion protein purified by monoclonal antibody affinity columns, no neutralizing or virus-binding antibodies were detected, but antibodies binding to denatured VP7 were present.