ABSTRACT
We have sequenced on both strands a 40,257 bp fragment located near the left telomere of chromosome X of Saccharomyces cerevisiae. The sequenced segment contains 21 open reading frames (ORFs) at least 100 amino acids long. Five of the ORFs correspond to known amino acid sequences: two hypothetical proteins in the subtelomeric Y' repeat region of 65.4 and 12.8 KDa, the cytochrome B pre-mRNA processing CBP1 protein, the mitochondrial nuclease NUC1 and the CRT1 protein. Of the 16 remaining ORFs, eight show highest homologies with the S. cerevisiae hexose transporters family (two ORFs), the yeast alpha-glucosidase (two ORFs), the yeast PEP1 precursor, the Escherichia coli galactoside O-acetyltransferase, the S. cerevisiae 137.7 KDa protein located in the Y' region and a protein of unknown function of Schizosaccharomyces pombe. Finally, eight of the ORFs exhibit no significant similarity with any amino acid sequences described in data banks. DNA sequence comparison has revealed the presence of different repeated elements characteristic of yeast chromosome ends. Disruption studies have been performed on two ORFs encoding putative proteins of unknown function.
Subject(s)
Chromosomes, Fungal , DNA, Fungal/chemistry , Saccharomyces cerevisiae/genetics , Telomere , Base Sequence , Molecular Sequence Data , Open Reading FramesABSTRACT
The complete DNA sequence of the yeast Saccharomyces cerevisiae chromosome XI has been determined. In addition to a compact arrangement of potential protein coding sequences, the 666,448-base-pair sequence has revealed general chromosome patterns; in particular, alternating regional variations in average base composition correlate with variations in local gene density along the chromosome. Significant discrepancies with the previously published genetic map demonstrate the need for using independent physical mapping criteria.
Subject(s)
Chromosomes, Fungal , DNA, Fungal , Saccharomyces cerevisiae/genetics , Base Sequence , Chromosome Mapping , Fungal Proteins/genetics , Open Reading FramesABSTRACT
A 20.5 kb DNA fragment from the left arm of chromosome XI of Saccharomyces cerevisiae has been sequenced and analysed. Thirteen open reading frames (ORFs) for proteins longer than 100 amino acids were discovered. Among them, two are the known genes MRP49 and TPK3; two others encode proteins which show strong similarity with a yeast putative protein kinase and a yeast choline transport protein; one other shows weaker similarity with a yeast Ca2+/calmodulin-dependent protein kinase. Moreover, two putative proteins encoded by ORFs located in the sequenced fragment are closely similar to non-yeast proteins: the Caenorhabditis elegans elongation factor 2 and a glutamic acid-rich protein of Plasmodium falciparum.
Subject(s)
Chromosomes, Fungal , Genes, Fungal/genetics , Open Reading Frames/genetics , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Base Sequence , Chromosome Mapping , Cyclic AMP-Dependent Protein Kinases/genetics , Mitochondrial Proteins , Molecular Sequence Data , Ribosomal Proteins/genetics , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
We have completely sequenced on both strands a continuous DNA segment of 36.2 kb located on the left arm of Saccharomyces cerevisiae chromosome XI. Sequence analysis reveals the presence of 20 open reading frames (ORFs) at least 100 amino acids long. Five of these ORFs correspond to known genes; five others show homology with known proteins; the ten remaining ORFs identified show no detectable homology with other protein sequences contained in data banks and may represent new biological functions.
Subject(s)
Chromosomes, Fungal , Genes, Fungal/genetics , Open Reading Frames/genetics , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Aminopeptidases/genetics , Chromosome Mapping , Molecular Sequence Data , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
We have determined the nucleotide sequence of a segment from chromosome III of Saccharomyces cerevisiae extending over 7.9 kb between the PGK1 and CRY1 loci. The fragment contains seven open reading frames, YCR241, YCR242, YCR243, YCR244, YCR245, YCR246 and YCR247, of more than 70 codons. The study of the effects of a global disruption of YCR242, YCR243, YCR244, YCR245 and YCR247 shows that they are not essential for growth and division.
Subject(s)
Chromosomes, Fungal , DNA, Fungal/genetics , Open Reading Frames , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Base Sequence , Codon , Fungal Proteins/genetics , Genes, Fungal , Molecular Sequence DataABSTRACT
The YDp plasmids (Yeast Disruption plasmids) are pUC9 vectors bearing a set of yeast gene disruption cassettes, all uniform in structure and differing only in the selectable marker used (HIS3, LEU2, LYS2, TRP1 or URA3). The markers, surrounded by translational termination codons, are embedded in the slightly modified sequence of the pUC9 multiple cloning sites.
