ABSTRACT
Agonists of glutamate ionotropic (NMDA) and metabotropic receptors (mGluRI and mGluRIII) had the regulatory effect on ADP-ribosyl cyclase/CD38 activity in cerebellar granular cells of newborn rats. Perinatal hypoxic-ischemic brain injury was followed by dysregulation of this mechanism.
Subject(s)
ADP-ribosyl Cyclase/metabolism , Excitatory Amino Acid Agonists/metabolism , Hypoxia-Ischemia, Brain/pathology , Receptors, Ionotropic Glutamate/agonists , Receptors, Metabotropic Glutamate/agonists , Signal Transduction , ADP-ribosyl Cyclase 1/metabolism , Animals , Cerebellum/drug effects , Cerebellum/metabolism , Female , Hypoxia-Ischemia, Brain/metabolism , Male , Membrane Glycoproteins/metabolism , N-Methylaspartate/pharmacology , Neurons/metabolism , RatsABSTRACT
A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. Clones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The genes of one beta-1,4-glucanase and one xylanase are probably located in the same region of A.thermophilum chromosome.
