Mitigation of acidified salmon rivers - effects of liming on young brown trout Salmo trutta.

In southern Norway, 22 acidified rivers supporting anadromous salmonids were mitigated with lime to improve water quality and restore fish populations. In 13 of these rivers, effects on Salmo trutta and Salmo salar densities were monitored over 10-12 years, grouped into age 0 and age ≥ 1 year fish. These rivers had a mean annual discharge of between 4·9 and 85·5 m3 s-1 , and six of them were regulated for hydro-power production. Salmo salar were lost in six of these rivers prior to liming, and highly reduced in the remaining seven rivers. Post-liming, S. salar became re-established in all six rivers with lost populations, and recovered in the seven other rivers. Salmo trutta occurred in all 13 study rivers prior to liming. Despite the improved water quality, both age 0 and age ≥ 1 year S. trutta densities decreased as S. salar density increased, with an average reduction of >50% after 10 years of liming. For age 0 year S. trutta this effect was less strong in rivers where S. salar were present prior to liming. In contrast, densities of S. trutta increased in unlimed streams above the anadromous stretches in two of the rivers following improved water quality due to natural recovery. Density increases of both age 0 and age ≥ 1 year S. salar showed a positive effect of river discharge. The results suggest that the decline in S. trutta density after liming is related to interspecific resource competition due to the recovery of S. salar. Thus, improved water quality through liming may not only sustain susceptible species, but can have a negative effect on species that are more tolerant prior to the treatment, such as S. trutta.

The effects of sexual selection on life-history traits: an experimental study on guppies.

Sexual selection is often prevented during captive breeding in order to maximize effective population size and retain genetic diversity. However, enforcing monogamy and thereby preventing sexual selection may affect population fitness either negatively by preventing the purging of deleterious mutations or positively by reducing sexual conflicts. To better understand the effect of sexual selection on the fitness of small populations, we compared components of female fitness and the expression of male secondary sexual characters in 19 experimental populations of guppies (Poecilia reticulata) maintained under polygamous or monogamous mating regimes over nine generations. In order to generate treatments that solely differed by their level of sexual selection, the middle-class neighbourhood breeding design was enforced in the monogamous populations, while in the polygamous populations, all females contributed similarly to the next generation with one male and one female offspring. This experimental design allowed potential sexual conflicts to increase in the polygamous populations because selection could not operate on adult-female traits. Clutch size and offspring survival showed a weak decline from generation to generation but did not differ among treatments. Offspring size, however, declined across generations, but more in monogamous than polygamous populations. By generation eight, orange- and black-spot areas were larger in males from the polygamous treatment, but these differences were not statistically significant. Overall, these results suggest that neither sexual conflict nor the purging of deleterious mutation had important effects on the fitness of our experimental populations. However, only few generations of enforced monogamy in a benign environment were sufficient to negatively affect offspring size, a trait potentially crucial for survival in the wild. Sexual selection may therefore, under certain circumstances, be beneficial over enforced monogamy during captive breeding.

Artificial selection on allometry: change in elevation but not slope.

To what extent within-species (static) allometries constitute a constraint on evolution is the subject of a long-standing debate in evolutionary biology. A prerequisite for the constraint hypothesis is that static allometries are hard to change. Several studies have attempted to test this hypothesis with artificial-selection experiments, but their results remain inconclusive due to various methodological issues. Here, we present results from an experiment in which we selected independently on the slope and the elevation of the allometric relationship between caudal-fin size and body size in male guppies (Poecilia reticulata). After three episodes of selection, the allometric elevation (i.e. intercept at constant slope) had diverged markedly between the lines selected to increase or decrease it, and showed a realized heritability of 50%. In contrast, the allometric slope remained unaffected by selection. These results suggest that the allometric elevation is more evolvable than the allometric slope, this latter representing a potential constraint on adaptive trait evolution. To our knowledge, this study is the first artificial-selection experiment that directly tests the evolvability of static allometric slopes.

Geographical variation in allometry in the guppy (Poecilia reticulata).

Variation in static allometry, the power relationship between character size and body size among individuals at similar developmental stages, remains poorly understood. We tested whether predation or other ecological factors could affect static allometry by comparing the allometry between the caudal fin length and the body length in adult male guppies (Poecilia reticulata) among populations from different geographical areas, exposed to different predation pressures. Neither the allometric slopes nor the allometric elevations (intercept at constant slope) changed with predation pressure. However, populations from the Northern Range in Trinidad showed allometry with similar slopes but lower intercepts than populations from the Caroni and the Oropouche drainages. Because most of these populations are exposed to predation by the prawn Macrobrachium crenulatum, we speculated that the specific selection pressures exerted by this predator generated this change in relative caudal fin size, although effects of other environmental factors could not be ruled out. This study further suggests that the allometric elevation is more variable than the allometric slope.

Temporal change in inbreeding depression in life-history traits in captive populations of guppy (Poecilia reticulata): evidence for purging?

Inbreeding depression, which generally affects the fitness of small populations, may be diminished by purging recessive deleterious alleles when inbreeding persists over several generations. Evidence of purging remains rare, especially because of the difficulties of separating the effects of various factors affecting fitness in small populations. We compared the expression of life-history traits in inbred populations of guppy (Poecilia reticulata) with contemporary control populations over 10 generations in captivity. We estimated inbreeding depression as the difference between the two types of populations at each generation. After 10 generations, the inbreeding coefficient reached a maximum value of 0.56 and 0.16 in the inbred and control populations, respectively. Analysing changes in the life-history traits across generations showed that inbreeding depression in clutch size and offspring survival increased during the first four to six generations in the populations from the inbred treatment and subsequently decreased as expected if purging occurred. Inbreeding depression in two other traits was weaker but showed similar changes across generations. The loss of six populations in the inbred treatment indicates that removal of deleterious alleles also occurred by extinction of populations that presumably harboured high genetic load.

The effect of air bubbles on rabbit blood brain barrier.

Several investigators have claimed that the blood brain barrier (BBB) may be broken by circulating bubbles, resulting in brain tissue edema. The aim of this study was to examine the effect of air bubbles on the permeability of BBB. Three groups of 6 rabbits were infused an isoosmotic solution of NaCl w/macrodex and 1% Tween. The solution was saturated with air bubbles and infused at rates of 50-100 ml hr(-1), a total of 1.6, 3.3, or 6.6 ml in each group, respectively. Two groups, each consisting of 6 rabbits, served as controls; one was infused by a degassed isoosmotic NaCl solution and one was sham-operated. All animals were left for 30 min before they were sacrificed. Specific gravity of brain tissue samples was determined using a brombenzene/kerosene gradient column, where a decrease in specific gravity indicates local brain edema. Specific gravity was significantly lower for left (P = 0.037) and right (P = 0.012) hemisphere white matter and left (P = 0.0015) and right (P = 0.002) hemisphere gray matter for the bubble-infused animals compared to the sham-operated ones. Infusion of degassed NaCl solution alone affected white left (P= 0.011) and right (P= 0.013), but not gray matter of both hemispheres. We speculate that insufficient degassing of the fluid may cause the effect of NaCl solution on the BBB of the white matter, indicating that the vessels of the white matter are more sensitive to gas bubbles than gray matter. Increasing the number of infused bubbles had no further impact on the development of cerebral edema, indicating that a threshold value was reached already at the lowest concentration of bubbles.

Anthropometry of norwegian light industry and office workers.

Several of the Directives and Standards developed by CEN, e.g. those dealing with Machine Safety and Personal Protective Equipment, depend on anthropometric information. For Norway, anthropometric data relevant for these standards have either been lacking or are very old. Two hundred males and 199 female employees of light industry and office work, between 20 and 39 years of age were studied. Body mass plus 29 different body dimensions were measured. Mean age of the total male and female populations were 30.0+/-5.6 and 30.0+/-5.4 years, respectively. Mean stature for males was 1796+/-66 mm, while the corresponding value for females was 1661+/-61 mm. Body weights for the two populations averaged 78.2+/-11.1 and 63.4+/-9.3 kg, respectively. There were no significant differences in stature between the two age groups, neither in males nor in females. In body mass, males 30-39 years of age had significantly higher body mass than the 20-29 years old, while this was not the case in females.

Muscle fibre type and dimension in genetically obese and lean Zucker rats.

Skeletal muscle structure and morphology may be altered in obesity. To study this further, muscles from six genetically obese (fa/fa) and six normal male rats were examined at 15 weeks of age. The gluteus medius, vastus lateralis and rectus abdominis muscles were dissected out and stained for histochemical fibre typing. In addition the fibre cross-sectional area was measured on a graphic tablet. The proportion of fast-twitch fibres was larger in the vastus lateralis and rectus abdominis muscles of the obese rats (P < 0.01); no difference was seen for the gluteus medius muscle. For the normal rats the cross-sectional area of the fast-twitch fibres was 2-3 times larger than the area of slow-twitch fibres in the same muscle. The cross-sectional area of the fast-twitch fibres in the obese rats was 40-47% less than in the control animals (P < 0.003), while no difference between the two groups was found for the slow-twitch fibre area. The data thus suggest that in the genetically obese rats the development of fast-twitch fibres was primarily affected. Moreover, in these animals some muscles may be more affected than others.

Effects of water temperature on performance: a practical evaluation of a neutral buoyancy facility.

Manual and cognitive performance of two female and four male divers was evaluated in "cold" and "warm" water in a Neutral Buoyancy Facility (NBF). A test battery of six manual and cognitive tests was applied in a fixed sequence in three separate, 3-h dives: Dive 1) Water temp.: 18-19 degrees C, wet suit 3-5 mm thick; Dive 2) Water temp.: 32-33 degrees C bathing suit and T-shirt; and Dive 3) Water temp.: 18-19 degrees C, tailor made wet suit 6.5 mm thick. No significant differences in performance between the three conditions were recorded. Mean rectal temperatures decreased by 1 degree C in all dive conditions, except in females in Dive 2. Ventilation was significantly higher in Dive 1 than in Dives 2 and 3. Thermal discomfort was reported only after 2 h in Dive 1. We suggest that support divers may work safely, comfortably and effectively for at least 2 h in water of 18-19 degrees C, if dressed in thermal protective wet suits.

[Women and diving]. / Kvinner og dykking.

PIP: In recent years, athletic diving by women has increased substantially, yet little is known regarding the effects on women's bodies. Of special concern are the effects from the rapid depressurization when surfacing. There is much evidence to suggest that because women have a greater ration of fat to body weight than men, they are more likely to suffer from bends, and that it is more difficult to treat them once afflicted with this. Another question asked by women divers regards whether the use of oral contraceptives may increase the danger of getting bends disease. A study in 1981 of 610 women divers, of whom nearly half used oral contraceptives, revealed no correlation with bends, however. A 3rd question asked is whether decompression has any significant effect on the menstruation cycle, hormone patterns or ovulation. A study examining all these aspects found no such effects. The most serious problems from diving are believed to be faced by pregnant women, and especially by their fetuses. Studies in this area have mainly undertook to calculate the relative distribution of air bubbles and the oxygen-partial pressure in the blood of mother and fetus. Animal tests have shown the presence of air bubbles in the blood of fetuses, while the mother's blood remained free of them. One such study was done by subjecting pregnant lambs to a pressure-chamber experiment. Of 13 births, 3 were stillborn. This and other animal studies have suggested that the nearer to the time of birth that depressurization occurs, the greater is the danger to the fetus. Inquiry studies of women divers also seem to indicate some danger. In a study of 136 women who dived during pregnancy, 5.5% had given birth to babies with severe deformities, compared to women in a control group. The Undersea Medical Society, as well as a growing number of researchers, recommend that pregnant women avoid diving, since there is always risk of having to undertake a rapid surfacing and incurring bends symptoms and other complications as consequences.^ieng

Heterogeneity in the adrenomedullary storage of catecholamines, ATP, calcium and releasable dopamine beta-hydroxylase activity.

Bovine adrenomedullary granules were separated into two subfractions by isopycnic density centrifugation. A small subfraction (approximately 10% of the total population) was sedimented into 2.2 M sucrose while the main population (80% of the total) was recovered at the interphase between 1.6 and 2.2 M sucrose. The concentrations of catecholamine (CA) and calcium showed marked seasonal variations for both subfractions, with lowest levels in the spring and highest levels in the winter. Throughout the year the concentrations of CA and calcium were 2-3 times higher in the minor subpopulation which also accounted for an abundance of noradrenaline (NA); on average 68% NA of total CA, 6.6 mumol CA and 225 nmol calcium/mg protein. The two subpopulations stored CA in similar ratios to ATP and calcium; i.e. 30 mol CA: 4 mol ATP: 1 mol Ca2+, indicating storage of CA largely independent of an equivalent amount of ATP, at least during winter when CA storage was 3.3 and 9.9 mumol/mg protein in the major and minor subpopulation respectively. The two subpopulations differed significantly in ratio of releasable dopamine beta-hydroxylase (DBH) activity per mole CA due to insignificant differences in specific activity of releasable DBH (0.4 unit/mg protein). The results show: (1) that the adrenomedullary granules are heterogeneous with respect to releasable activity of DBH per mole CA and subject to considerable seasonal variations; (2) a large portion of the NA-storing granules has a high concentration of releasable constituents; (3) both adrenaline (A)- and NA-storage are closely associated with that of calcium and occur in excess of that balanced by equivalent amounts of ATP.

Energy metabolism in different human skeletal muscles during voluntary isometric contractions.

The energy turnover in contracting skeletal muscle was studied by measuring the rate of temperature rise during voluntary, isometric contractions and circulatory arrest in M. soleus, M. sacrospinalis and M. biceps brachii in 14 males, by thermoelements inserted in the muscles. A linear relationship between rate of temperature rise and force intensity given as per cent of maximal voluntary contraction (MVC) was demonstrated in biceps (r = 0.95), but not so clearly confirmed in soleus (r = 0.73). Muscle biopsies were taken from the same muscles and fibre type distribution was determined histochemically by staining for ATPase. The rate of heat production at MVC showed positive correlation to the percentage of fast twitch (FT) fibres in the muscles (r = 0.90). Linear extrapolation indicates that the maximal energy turnover in human FT fibres is approximately six times that of slow twitch (ST) fibres during voluntary isometric contractions.