How high salt shock affects performance and membrane fouling characteristics of a halophilic membrane bioreactor used for treating hypersaline wastewater.

In the present study, the effect of short-term salt shocks (13% and 20%) on the performance of a halophilic MBR bioreactor used to treat a hypersaline (5% salt) synthetic wastewater was considered. 13% and 20% salt shocks resulted in a transient and permanent decrease in chemical oxygen demand removal efficiency, respectively which could be correlated with soluble microbial products (SMP) concentration and specific oxygen uptake rate values of the halophilic population. DNA leakage tests suggested that both 13% and 20% short-term salt shocks resulted in some cell structural damage. During both 13% and 20% salt shocks mixed liquor SMP, extracellular polymeric substances (EPS), zeta potential and endogenous respiration increased while relative hydrophobicity, EPSp/EPSc and exogenous respiration decreased; in both cases, however, the pre-shock values for these parameters were restored after the removal of the salt shock. 13% salt shock resulted in a transient increase in the membrane fouling rate and a permanent rise in total membrane resistance (Rt). On the other hand, both membrane fouling rate and Rt increased during 20% salt shock. Membrane fouling rate initially reduced after the 20% salt shock removal but after 5 days a "TMP jump" occurred. The latter was caused by the higher steady state SMPc and SMPp concentrations after removal of 20% salt shock compared to pre-shock values. This might have either resulted in a decrease in critical flux or an increase in local flux above critical flux in some parts of the membrane. The contribution of cake layer resistance to overall membrane resistance increased after the 13% and 20% salt shocks. The findings of the present study reveal the robustness of halophilic MBRs against salt shocks in the treatment of hypersaline wastewater. However, in cases of very high salt shocks, appropriate membrane fouling reduction strategies should be carried out during its operation.

Integrating electrocoagulation process with up-flow anaerobic sludge blanket for in-situ biomethanation and performance improvement.

In this study, the integration of the electrocoagulation (EC) process with anaerobic digestion as a novel in-situ biomethanation approach was considered for the first time. As a result of this integration (iron electrodes, current density of 1.5 mA/cm2 and an exposure mode of 10-min-ON/ 30-min-OFF), the carbon dioxide content of biogas reached below 2%. Also, the methane production rate improved by 18.0 ± 0.4%, whereas the removal efficiencies of chemical oxygen demand, turbidity, phosphate, and sulfate increased by 12.0 ± 1.5%, 30.7 ± 1.7%, > 99%, and 75.7%, respectively. Anaerobic granular sludge characteristics were also improved. Moreover, the EC process stimulated growth and quantity of functional microorganisms, especially Acinetobacter in bacterial and Methanobacterium in archaeal community. Methane concentration, however decreased due to possible excess hydrogen production. The application of the biogas as bio-hythane, and the optimization of the hybrid bioreactor to decrease hydrogen production, are possible avenues for further research.

The effect of temperature and styrene concentration on biogas production and degradation characteristics during anaerobic removal of styrene from wastewater.

In the current study, styrene was removed anaerobically from wastewaters at temperatures of 35 ℃, 25 ℃, and 15 ℃ and concentration range of 20-150 ppm in the presence of ethanol as a co-substrate and co-solvent. Maximum styrene removal of 93% was achieved at 35 ℃. The volatilization of styrene was negligible at about 2% at all experimented temperatures. The average special methane yield (SMY) at 35 ℃ was 4.14- and 225-times higher than that of at T = 25 ℃ and T = 15 ℃, respectively, but no methane was produced in the absence of ethanol. The proteins content of the soluble microbial product (SMP) and extracellular polymeric substance (EPS) was much higher than the carbohydrate content. At styrene concentration > 80 ppm, SMY, SMP, and EPS dropped sharply. The results confirmed the well performance of anaerobic microorganisms in removing styrene from wastewater and biogas production at mesophilic condition.

Considering a membrane bioreactor for the treatment of vegetable oil refinery wastewaters at industrially relevant organic loading rates.

The present study aims to shed more light on the use of membrane bioreactors (MBRs) for the treatment of vegetable oil refinery wastewaters (VORWs). A MBR was operated for 157 days in which it was fed with real VORW of varying composition at a range of organic loading rates (0.20 ± 0.05-3.79 ± 0.29 kg COD m-3 day-1). The hitherto unconsidered fate of VORW constituents through the biological process was followed using gas chromatography/mass spectrometry analysis. This analysis revealed that only 19% of the identified feed constituents remained in the MBR effluent whereas ten new compounds were formed. Linear correlation analysis attributed the effluent residual COD to soluble microbial products (SMP) and non-readily biodegradable recalcitrant oily compounds. Trend of change of MLSS, mixed liquor viscosity and SMP with increasing OLR suggested that when MBR is operated under industrial conditions for the VORW treatment, the mixed liquor fouling propensity potentially increases with increasing OLR in the range studied.

Gaining deeper insights into the bioflocculation process occurring in a high loaded membrane bioreactor used for the treatment of synthetic greywater.

In the present study, a high loaded membrane bioreactor (HL-MBR) operated at a hydraulic retention time (HRT) of 1.5 h, and three different sludge retention times (SRTs) in the range of 0.5-2 days, was used for the treatment of synthetic greywater. The chemical oxygen demand (COD) removal efficiency of the system was in the range 87-89% at all SRTs. Bioflocculation efficiency (defined as the percentage of suspended COD in the concentrate stream), COD bio-oxidation, total extracellular polymeric substances (EPS), tightly bound (TB) EPS and the ratio of EPS protein (EPSp) to carbohydrate (EPSc) increased when SRT was increased from 0.5 to 2 days. Sludge supernatant soluble microbial products (SMP) increased with increase in SRT from 0.5 to 2 days, while the effluent SMP was negligible. Particle size distribution analyses revealed a bimodal distribution at an SRT of 0.5 days, and normal distributions at other SRTs. Furthermore, depending on the value of the F/M ratio, different SRTs in the range of 0.5-2 days had either positive or negative effects on the mean particle size. Linear correlation analyses were performed using the data obtained during both transient and steady-state operations of the HL-MBR system. TB-EPS and EPSp showed strong correlations with the biofloccultaion efficiency, whereas loosely bound (LB) EPS correlated with soluble COD removal. TB-EPS and EPSc had negative correlations with the energy recovery potential of the system. The trend of change of parameters affecting membrane fouling intensity with SRT suggested that, in the range studied, the lowest rate of membrane fouling would be expected at SRT of 0.5 days.

Quantifying the organic content of saline wastewaters: Is chemical oxygen demand always an achievable parameter?

The study presented in this paper takes a comprehensive approach to the measurement of the COD of saline industrial wastewaters taking into account both their widely varying salinity levels and the substantial interference of chloride with the conventional method of COD measurement. To this end, three approaches for combating the chloride interference associated with the measurement of COD using the conventional method were considered. The dilution of saline samples prior to analysis yielded reasonably accurate COD results as long as the COD after dilution was 40 mg L-1 or above. In the second approach, the previously reported modifications of the standard method were stretched to their practical limits (increasing HgSO4 to 130 g L-1 and decreasing K2Cr2O7 to 1.022 g L-1) accompanied by prior addition of HgSO4:Cl- at a ratio of 20:1 combined with chloride interference error estimation. This brought about an increase in chloride interference threshold of the standard method to 42.5 g L-1, which is considerably higher than previous reports. Since some raw or treated saline industrial wastewaters have a combination of chloride and COD concentration which makes the first two approaches inapplicable, the approach of chloride removal from the sample via a modification of DIN 38409-H41-2 and subsequent measurement of COD using a slight variation of the closed reflux standard method was also considered. Fairly accurate COD determinations for samples with chloride concentrations up to 148.6 and 182 g L-1 for COD contents of 50 and 900 mg L-1, respectively were achieved. However, excessive precipitation of the desalination reaction products made the method inapplicable to samples with chloride concentrations above 182 g L-1.

Identification of dust storm origin in South -West of Iran.

BACKGROUND: Deserts are the main sources of emitted dust, and are highly responsive to wind erosion. Low content of soil moisture and lack of vegetation cover lead to fine particle's release. One of the semi-arid bare lands in Iran, located in the South-West of Iran in Khoozestan province, was selected to investigate Sand and Dust storm potential. METHODS: This paper focused on the metrological parameters of the sampling site, their changes and the relationship between these changes and dust storm occurrence, estimation of Reconaissance Drought Index, the Atterberg limits of soil samples and their relation with soil erosion ability, the chemical composition, size distribution of soil and airborne dust samples, and estimation of vertical mass flux by COMSALT through considering the effect of saffman force and interparticle cohesion forces during warm period (April-September) in 2010. The chemical compositions are measured with X-ray fluorescence, Atomic absorption spectrophotometer and X-ray diffraction. The particle size distribution analysis was conducted by using Laser particle size and sieve techniques. RESULTS: There was a strong negative correlation between dust storm occurrence and annual and seasonal rainfall and relative humidity. Positive strong correlation between annual and seasonal maximum temperature and dust storm frequency was seen. Estimation of RDIst in the studied period showed an extremely dry condition. Using the results of particle size distribution and soil consistency, the weak structure of soil was represented. X-ray diffraction analyses of soil and dust samples showed that soil mineralogy was dominated mainly by Quartz and calcite. X-ray fluorescence analyses of samples indicated that the most important major oxide compositions of the soil and airborne dust samples were SiO2, Al2O3, CaO, MgO, Na2O, and Fe2O3, demonstrating similar percentages for soil and dust samples. Estimation of Enrichment Factors for all studied trace elements in soil samples showed Br, Cl, Mo, S, Zn, and Hg with EF values higher than 10. CONCLUSION: The findings, showed the possible correlation between the degree of anthropogenic soil pollutants, and the remains of Iraq-Iran war. The results expressed sand and dust storm emission potential in this area, was illustrated with measured vertical mass fluxes by COMSALT.

Sequential anaerobic-aerobic biological treatment of colored wastewaters: case study of a textile dyeing factory wastewater.

In the present study the feasibility of the use of a bacterial batch sequential anaerobic-aerobic process, in which activated sludge was used in both parts of the process, for pretreatment of wastewater generated by a textile dyeing factory has been considered. Activated sludge used in the process was obtained from a municipal wastewater treatment plant and adapted to real dyeing wastewater using either an anaerobic-only or an anaerobic-aerobic process over a period of 90 days. The use of activated sludge adapted using the anaerobic-aerobic process resulted in a higher overall decolorization efficiency compared to that achieved with activated sludge adapted using the anaerobic-only cycles. Anaerobic and aerobic periods of around 34 and 22 hours respectively resulted in an effluent with chemical oxygen demand (COD) and color content which met the standards for discharge into the centralized wastewater treatment plant of the industrial estate in which the dyeing factory was situated. Neutralization of the real dyeing wastewater and addition of carbon source to it, both of which results in significant increase in the cost of the bacterial treatment process, was not found to be necessary to achieve the required discharge standards.

The Influence of Sugar Cane Bagasse Type and Its Particle Size on Xylose Production and Xylose-to-Xylitol Bioconversion with the Yeast Debaryomyces hansenii.

In the present study, the effect of the type of sugar cane bagasse (non-depithed or depithed) and its particle size on the production of xylose and its subsequent fermentation to xylitol by Debaryomyces hansenii CBS767 was investigated using a full factorial experimental design. It was found that the particle size range and whether bagasse was depithed or not had a significant effect on the concentration and yield of xylose in the resulting hemicellulose hydrolysate. Depithed bagasse resulted in higher xylose concentrations compared to non-depithed bagasse. The corresponding detoxified hemicellulose hydrolysates were used as fermentation media for the production of xylitol. The hemicellulose hydrolysate prepared from depithed bagasse also yielded meaningfully higher xylitol fermentation rates compared to non-depithed bagasse. However, in the case of non-depithed bagasse, the hemicellulose hydrolysate prepared from larger particle size range resulted in higher xylitol fermentation rates, whereas the effect in the case of non-depithed bagasse was not pronounced. Therefore, depithing of bagasse is an advantageous pretreatment when it is to be employed in bioconversion processes.

Treatment of hypersaline produced water employing a moderately halophilic bacterial consortium in a membrane bioreactor: effect of salt concentration on organic removal performance, mixed liquor characteristics and membrane fouling.

In this study the organic pollutant removal performance and the mixed liquor characteristics of a membrane bioreactor (MBR), employing a moderately halophilic bacterial consortium, for the treatment of hypersaline synthetic produced water containing 100-250 g L(-1) NaCl were considered. The COD and oil and grease (O&G) removal efficiencies in the range 81.6-94.6% and 84.8-94.0% respectively and MBR effluent turbidity lower than 2NTU were achieved. There was no pronounced membrane fouling at any salt concentration. O&G accumulation (less than 11% of the influent O&G) occurred in the mixed liquor at all salt concentrations, but biodegradation was identified as the major organic removal mechanism. With increasing salt concentration, initially increase in SVI and later formation of oil/biomass bodies took place but due to the presence of the membrane biomass washout did not occur. The mixed liquor was pseudoplastic and the apparent viscosity and flow behavior index generally increased with salt concentration.

The study of organic removal efficiency and halophilic bacterial mixed liquor characteristics in a membrane bioreactor treating hypersaline produced water at varying organic loading rates.

In this study the organic pollutant removal performance and the mixed liquor characteristics of a membrane bioreactor (MBR), employing a halophilic bacterial consortium, for the treatment of hypersaline synthetic produced water - at varying organic loading rates (OLR) from 0.3 to 2.6 kg CODm(-3)d(-1) - were considered. The oil and grease (O&G) and COD removal efficiency were 95-99% and 83-93%, respectively with only transient O&G (mainly polycyclic aromatic hydrocarbons) and soluble microbial products accumulation being observed. With increasing OLR, in the range 0.9-2.6 kg COD m(-3)d(-1), as a result of change in both extracellular polymeric substances (EPS) and zeta potential, bioflocculating ability improved but the compressibility of the flocs decreased resulting in the occurrence of EPS bulking at the highest OLR studied. The latter resulted in a change in the rheology of the mixed liquor from Newtonian to non-Newtonian and the occurrence of significant membrane fouling.

The effect of hydraulic retention time on the performance and fouling characteristics of membrane sequencing batch reactors used for the treatment of synthetic petroleum refinery wastewater.

The use of membrane sequencing batch reactors, operated at HRT of 8, 16 and 24h, was considered for the treatment of a synthetic petroleum wastewater. Increase in HRT resulted in statistically significant decrease in MLSS. Removal efficiencies higher than 97% were found for the three model hydrocarbon pollutants at all HRTs, with air stripping making a small contribution to overall removal. Particle size distribution (PSD) and microscopic analysis showed reduction in the protozoan populations in the activated sludge with decreasing HRT. PSD analysis also showed a higher proportion of larger and smaller sized particles at the lowest HRT. The rate of membrane fouling was found to increase with decreasing HRT; SMP, especially carbohydrate SMP, and mixed liquor apparent viscosity also showed a pronounced increase with decreasing HRT, whereas the concentration of EPS and its components decreased. FTIR analysis identified organic compounds as the main component of membrane pore fouling.

Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions.

The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.