ABSTRACT
We compared the most reliable and practical laboratory methods for the quantitative determination of serum C-reactive protein that is polistyrene latex particles together with purified anti-C-reactive protein rabbit globulin, the precipitation test using anti-C-reactive protein serum and finally, radial immunodiffusion on agar-gel plates. We examined 163 serum specimens from ambulatory patients and from various hospitalized patients in Istituti Clinici di Perfezionamento of Milan (Italy). All the methods tested showed a good qualitative discrimination for serum C-reactive protein presence or absence at pathological levels. We also compared the two latex methods. In conclusion, radial immunodiffusion confirmed to be the best method for accurate C-reactive protein dosage during inflammatory and degenerative processes.
Subject(s)
C-Reactive Protein/analysis , Latex Fixation Tests , Chemical Precipitation/methods , Evaluation Studies as Topic , Female , Humans , Immunodiffusion , MaleABSTRACT
The TAS of 211 normo lipemic patients and of 84 hyperlipemic patients has been determined by the tube dilution hemolysis method of Rantz and Randall, the Aso Quantum hemolytic method (I.S.V.T. "Sclavo" S.p.A., Siena) and methods with streptolysin O antigen coated on the surface of biologically inert latex particles. A good correlation has been shown between all the analytical techniques considered and, moreover, the incidence of wrong positives and negatives has been showed in comparison with the hemolytic method by Rantz and Randall, considered test of reference. Finally, the greater influence of cholesterol on the classic hemolytic system, compared with the latex technique in causing an unspecific increase of TAS is proposed; this is evaluated before and after treatment of the hyperlipemic samples with ether.
