[Comparative study of the immunogenicity of various forms of Naja oxiana cardiotoxin]. / Sravnitel'noe izuchenie immunogennosti razlichnykh form kardiotoksina sredneaziatskoi kobry.

Comparative study of the immune sera from rabbits immunized with both formol and native cardiotoxins, cardiotoxin incorporated into sphingomyelin-phosphatidylcholine-cholesterol liposome stabilized with osmium tetroxide, and a cardiotoxin-succinylated bovine albumin conjugate was performed. The latter produced antibodies identified by immunodiffusion assay. The highest survival was observed in animals treated with antiserum to cardiotoxin-albumin conjugate, followed by antitoxin to combination of formol and native cardiotoxins. Antiserum to cardiotoxin in liposome was ineffective.

[Detection of lipopeptide complexes in lipid fractions of Proteus mirabilis]. / Obnaruzhenie lipopeptidnykh kompleksov v lipidnykh fraktsiiakh Proteus mirabilis.

Covalently bonded complexes of phospholipids with peptides were detected in the lipid fractions of Proteus mirabilis. The lipopeptides were developed with ninhydrin after treating thin-layer chromatograms with H1 or 0.1 N HCl at 100 degrees C for 4 hours. The complexes can be chromatographed in silicic acid unfixed layers using neutral or weakly acidic systems of solvents. The complexes break down in layers fixed with gypsum or treated with diluted alkali solutions. The complexes can contain several phospholipid molecules which make them soluble in organic solvents. The lowest proportion between phospholipids and peptide components was 4:1. Apparently, the complexes contain O-ester and pyrophosphoric bonds.

[Combination of lipid gas and thin-layer chromatography in the diagnosis of functional hepatic failure]. / Sochetannaia gazovaia i tonkosloinaia khromatografiia lipidov v diagnostike funktsional'noi nedostatochnosti pecheni.

Gas chromatography of total lipids from blood serum exhibits about 40% error in diagnosis of liver functional insufficiency. Combination of gas chromatography with quantitative thin-layer chromatography and subsequent gas chromatographic analysis of the lipid fractions obtained enabled to increase by 25% the efficiency of diagnoses not only of icteric but also of non-icteric forms of liver functional insufficiency. Simultaneous gas- thin-layer chromatographic analysis of total lipids will apparently improve the differential diagnosis of diseases accompanied by liver functional insufficiency; it permits also to study pathogenesis of impairments in lipid metabolism under conditions of liver functional insufficiency and of other pathologies.

[A quantitative method of determining lecithinases and lipases in microorganisms]. / Kolichestvennyi metod opredeleniia letsitinaz i lipaz u mikroorganizmov

The paper presents a method for quantitative assay of lecithinases and lipases in microorganisms. The method is based on the assessment of changes in the composition of lipid components of yolk media under the influence of corresponding enzymic systems of bacteria. The technique involved lipid extraction from yolk media after cultivation of the microorganisms tested, chromatographic separation by thin-layer chromatography on silicagel into individual fractions, development in iodine vapours and quantitative measurement of lipid components.

[Lipid composition of wild and histamine-dependent strains of Candida tropicalis D-2]. / Lipidinyi sostav dikogo i gistaminzavisimogo shtammov Candida tropicalis D-2

Lipid composition of prototropic and histidine-dependent strains of Candida tripicalis D-2 was studied by the method of gas and thin layer chromatography. Essential differences are shown in the quantitative composition of neutral lipids and fatty acids of the studied strains.