ABSTRACT
The efficiency of chemiluminescence immunoassay (CIA) used as a treponemal test in the serological diagnosis of syphilis was evaluated, by applying the serum samples of 219 patients screened for syphilitic infection. The efficiency of CIA was evaluated in two steps: (1) comparison of the results of tests using the IMMULITE 2000 Syphilis Screen and LIAISON Syphilis screen reagent kits; (2) that of the results of tests applying the IMMULITE 2000 Syphilis Screen and Recombi Best antipallidum-summary antibodies kits. A 100% correlation was found when comparing the results of tests employing the IMMULITE 2000 Syphilis Screen and DiaSorin LIAISON Syphilis Screen kits (n=66). Comparison of CIA using the IMMULITE 2000 Syphilis Screen kits versus that applying the Recombi Best antipallidum-summary antibodies kits (n=153) revealed 3 discordant samples, which amounted to 1.96% of the number of results. Evaluation of the impact of related microorganisms on the detection of T. pallidum antibodies (n= 140) with the IMMULITE 2000 Syphilis Screen kit indicated no interference or cross-reactivity.
Subject(s)
Syphilis/diagnosis , Antibodies, Bacterial/blood , Autoanalysis , Cross Reactions , Humans , Immunoenzyme Techniques , Syphilis Serodiagnosis/methods , Treponema pallidum/immunologySubject(s)
Diabetes Mellitus, Type 2/blood , Erythropoiesis , Reticulocytes , Erythrocyte Count , Female , Hematocrit , Humans , Male , Middle Aged , Reticulocytes/pathologyABSTRACT
Anemic syndrome rate in diabetic angiopathy reaching 1/3 in elderly patients, frequency increases after 70 years. The anemic syndrome rate in patients older 60 years suffering from the diabetes mellitus of the 1 type, complicated by "diabetic foot" syndrome and in cases with duration of the disease more than 10 years is elevated. In diabetic angiopathy anemia can be normo- or hypochromic or followed by aniso- or poykilocytosis. Among the causes of anemia in patients with diabetic angiopathies the important place is occupied by mediated by oxidative stress, endotoxicosis and protein glycation prolong hyperglycemia effect on the morphofunctional properties of the red blood cells.
