A single sex-linked dominant gene does not fully explain the codling moth's resistance to granulovirus.

BACKGROUND: In 2004, resistance to a commercial formulation of the Cydia pomonella granulovirus (CpGV) was identified in a field population of Cydia pomonella from an organic orchard in southern France. The genetic inheritance of this resistance was analysed in the resistant laboratory strain RGV. This strain was obtained using successive crosses between the resistant field population and a susceptible laboratory strain, SV, with selection for CpGV resistance at each generation. RESULTS: After eight generations of introgression of the resistant trait into SV, the RGV-8 strain exhibited 7000-fold higher resistance than SV. Mass-crossing experiments showed that resistance to CpGV is strongly dominant, sex dependent and under the control of a single major gene. However, the contribution of other genes is required to explain all of the data obtained in this study. These additional genes do not follow the laws of classical Mendelian transmission. CONCLUSION: Transmission of granulovirus resistance in the RGV-8 strain of C. pomonella cannot be fully explained by the effect of a locus located on the Z chromosome. The action of other factors needs to be considered.

Cydia pomonella granulovirus genotypes overcome virus resistance in the codling moth and improve virus efficiency by selection against resistant hosts.

Cydia pomonella granulovirus (CpGV) has been used for 15 years as a bioinsecticide in codling moth (Cydia pomonella) control. In 2004, some insect populations with low susceptibility to the virus were detected for the first time in southeast France. RGV, a laboratory colony of codling moths resistant to the CpGV-M isolate used in the field, was established with collection of resistant insects in the field followed by an introgression of the resistant trait into a susceptible colony (Sv). The resistance level (based on the 50% lethal concentrations [LC(50)s]) of the RGV colony to the CpGV-M isolate, the active ingredient in all commercial virus formulations in Europe, appeared to be over 60,000-fold compared to the Sv colony. The efficiency of CpGV isolates from various other regions was tested on RGV. Among them, two isolates (I12 and NPP-R1) presented an increased pathogenicity on RGV. I12 had already been identified as effective against a resistant C. pomonella colony in Germany and was observed to partially overcome the resistance in the RGV colony. The recently identified isolate NPP-R1 showed an even higher pathogenicity on RGV than other isolates, with an LC(50) of 166 occlusion bodies (OBs)/microl, compared to 1.36 x 10(6) OBs/microl for CpGV-M. Genetic characterization showed that NPP-R1 is a mixture of at least two genotypes, one of which is similar to CpGV-M. The 2016-r4 isolate obtained from four successive passages of NPP-R1 in RGV larvae had a sharply reduced proportion of the CpGV-M-like genotype and an increased pathogenicity against insects from the RGV colony.