Analysis of uncoated LPGs written in B-Ge doped fiber under proton irradiation for sensing applications at CERN.

In this contribution, a complete dissertation concerning the behavior of a Long Period Grating (LPG) inscribed in a B-Ge co-doped optical fiber by means of an excimer laser and exposed to proton irradiation during a recent extensive campaign performed at the European Organization for Nuclear Research (CERN) with a fluence of 4.4·1015 p∙cm-2 is provided. The experimental results have been thus combined for the first time to the best of our knowledge with numerical simulations in order to estimate the variations of the major parameters affecting the grating response during the ultra-high dose proton exposure. From the correlation between experimental and numerical analysis, the irradiation exposure was found to induce a maximal variation of the core effective refractive index of ~1.61·10-4, responsible of a resonance wavelength red shift of ~44 nm in correspondence of the highest absorbed radiation dose of 1.16 MGy. At the same time, a relevant decrease close to ~0.93·10-4 in the refractive index modulation pertaining to the grating was estimated, leading to a reduction of the resonant dip visibility of ~12 dB. The effect of the proton beam on the spectral response of the LPG device and on the optical fiber parameters was assessed during the relaxation phases, showing a partial recovery only of the wavelength shift without any relevant change in the dip visibility revealing thus a partial recovery only in the refractive index of the core while the reduction of the refractive index modulation observed during the irradiation remained unchanged.

Long period fiber grating nano-optrode for cancer biomarker detection.

We report an innovative fiber optic nano-optrode based on Long Period Gratings (LPGs) working in reflection mode for the detection of human Thyroglobulin (TG), a protein marker of differentiated thyroid cancer. The reflection-type LPG (RT-LPG) biosensor, coated with a single layer of atactic polystyrene (aPS) onto which a specific, high affinity anti-Tg antibody was adsorbed, allowed the label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot tests, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels. The system is characterized by a very high sensitivity and specificity allowing the ex-vivo detection of sub ng/ml concentrations of human Tg from needle washouts of fine-needle aspiration biopsies of thyroid nodule from different patients.