ABSTRACT
Genomic sequencing of two relapsing fever spirochaetes showed truncation of recA in Borrelia recurrentis, but not in Borrelia duttonii. RecA has an important role among bacteria; we investigated whether this characteristic was representative of B. recurrentis, or an artefact following in vitro cultivation. We sequenced recA directly from samples of patient with louse-borne relapsing fever (B. recurrentis) or tick-borne relapsing fever (B. duttonii). We confirmed the premature stop codon in seven louse-borne relapsing fever samples, and its absence from three tick-borne relapsing fever samples. Furthermore, specific signature polymorphisms were found that could differentiate between these highly similar spirochaetes.
Subject(s)
Borrelia/physiology , Rec A Recombinases/physiology , Relapsing Fever/microbiology , Borrelia/genetics , Borrelia/isolation & purification , Codon, Nonsense , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Ethiopia , Humans , Molecular Sequence Data , Polymorphism, Genetic , Rec A Recombinases/genetics , Sequence Analysis, DNA , TanzaniaABSTRACT
An erupted cystic complex odontoma's case is reported. Histological, radiographic and clinic/fractures were considered. Erupted odontomas are unusual lesions; we did not find similar case reported in the literature.
