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1.
Am J Pathol ; 174(3): 797-807, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19164508

ABSTRACT

Angiotensin-converting enzyme (ACE) inhibition induces glomerular repair in the Munich Wistar Frömter (MWF) rat, a model of spontaneous glomerular injury. In this study, we investigated whether this effect is related to changes in glomerular cell number, particularly of podocytes, which are progressively lost with age. MWF rats with advanced nephropathy were studied at both 40 weeks and after 20 weeks of observation either with or without treatment with the ACE inhibitor lisinopril. Forty-week-old Wistar rats were used as controls. In untreated MWF rats, proteinuria, hypertension, glomerulosclerosis, and renal function worsened, while lisinopril induced regression of both functional and structural changes. Despite glomerular hypercellularity in untreated MWF rats, the number of endothelial cells per glomerulus did not change, and podocyte number even decreased. ACE inhibition halted the progressive increase in glomerular cell number and enhanced endothelial cell volume density. Surprisingly, lisinopril not only halted age-related podocyte loss but also increased the number of glomerular podocytes above baseline, which was associated with an increased number of proliferating Wilms tumor 1-positive cells, loss of cyclin-dependent kinase inhibitor p27 expression, and increased number of parietal podocytes. These data indicate that ACE inhibition restructures glomerular capillary, primarily by restoring the podocyte population in this model of glomerular injury. Increased parietal podocyte number in lisinopril-treated MWF rats suggests that the remodeling of Bowman's capsule epithelial cells contributes to this effect.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/toxicity , Kidney Glomerulus/pathology , Lisinopril/therapeutic use , Podocytes/cytology , Animals , Blood Pressure/drug effects , Cell Division , Glomerulosclerosis, Focal Segmental/drug therapy , Glomerulosclerosis, Focal Segmental/prevention & control , Hypertension/drug therapy , Hypertension/prevention & control , Kidney Function Tests , Kidney Glomerulus/drug effects , Male , Proteinuria/drug therapy , Proteinuria/prevention & control , Rats , Rats, Inbred WF , Rats, Wistar
2.
Am J Pathol ; 168(4): 1073-85, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16565484

ABSTRACT

Ameliorating the function of the glomerular barrier to circulating proteins by blocking angiotensin II (Ang II) translates into less risk of progression toward end-stage renal failure in diabetic and nondiabetic nephropathies. However, the mechanisms underlying this barrier protection are not clear. Specialized contacts between adjacent podocytes are major candidate targets, and the actin cytoskeleton is emerging as a regulatory element. Here, we present data demonstrating that Ang II induced reorganization of F-actin fibers and redistribution of zonula occludens-1 (ZO-1) that is physically associated with actin in murine podocytes. These effects were paralleled by increased albumin permeability across podocyte monolayers. The F-actin stabilizer jasplakinolide prevented both ZO-1 redistribution and albumin leakage, suggesting that actin cytoskeleton rearrangement is instrumental to podocyte permselective dysfunction induced by Ang II. Changes in both F-actin and ZO-1 patterns were confirmed in glomeruli of rat isolated perfused kidneys on short infusion of Ang II, leading to increased protein excretion. Podocyte dysfunction was mediated by Ang II type 1 receptor and was partly dependent on Src kinase-phospholipase C activation. These data demonstrate that strategies aimed at stabilizing podocyte-podocyte contacts and targeting the relevant intracellular signal transduction are crucial to renoprotection.


Subject(s)
Angiotensin II/physiology , Intercellular Junctions/physiology , Membrane Proteins/metabolism , Phosphoproteins/metabolism , Podocytes/physiology , Actins/physiology , Angiotensin II/pharmacology , Animals , Cell Differentiation , Cells, Cultured , Cytoskeleton/physiology , Depsipeptides/pharmacology , Enzyme Activation , In Vitro Techniques , Kidney Glomerulus/cytology , Kidney Glomerulus/metabolism , Mice , Permeability , Podocytes/metabolism , Rats , Receptor, Angiotensin, Type 1/physiology , Serum Albumin, Bovine/metabolism , Signal Transduction , Type C Phospholipases/metabolism , Zonula Occludens-1 Protein , src-Family Kinases/metabolism
3.
Am J Pathol ; 168(1): 42-54, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16400008

ABSTRACT

Changes in podocyte number or density have been suggested to play an important role in renal disease progression. Here, we investigated the temporal relationship between glomerular podocyte number and development of proteinuria and glomerulosclerosis in the male Munich Wistar Fromter (MWF) rat. We also assessed whether changes in podocyte number affect podocyte function and focused specifically on the slit diaphragm-associated protein nephrin. Age-matched Wistar rats were used as controls. Estimation of podocyte number per glomerulus was determined by digital morphometry of WT1-positive cells. MWF rats developed moderate hypertension, massive proteinuria, and glomerulosclerosis with age. Glomerular hypertrophy was already observed at 10 weeks of age and progressively increased thereafter. By contrast, mean podocyte number per glomerulus was lower than normal in young animals and further decreased with time. As a consequence, the capillary tuft volume per podocyte was more than threefold increased in older rats. Electron microscopy showed important changes in podocyte structure of MWF rats, with expansion of podocyte bodies surrounding glomerular filtration membrane. Glomerular nephrin expression was markedly altered in MWF rats and inversely correlated with both podocyte loss and proteinuria. Our findings suggest that reduction in podocyte number is an important determinant of podocyte dysfunction and progressive impairment of the glomerular permselectivity that lead to the development of massive proteinuria and ultimately to renal scarring.


Subject(s)
Glomerulosclerosis, Focal Segmental/physiopathology , Membrane Proteins/metabolism , Podocytes/metabolism , Podocytes/ultrastructure , Animals , Blotting, Western , Disease Models, Animal , Glomerulosclerosis, Focal Segmental/metabolism , Glomerulosclerosis, Focal Segmental/pathology , Hypertension/etiology , Immunohistochemistry , Male , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Proteinuria/etiology , RNA, Messenger/analysis , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction
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