ABSTRACT
The present research is an appraisal of anatomical and ultrastructural alterations in aquatic fern, Ceratopteris pteridoides under cadmium (Cd) exposure. Plants were cultured hydroponically for 12 consecutive days in different Cd treatments: 10 µM L-1 (CDT1), 20 µM L-1 (CDT2), 40 µM L-1 (CDT3) and 60 µM L-1 (CDT4). Anatomical and ultrastructural changes of different vegetative tissues of C. pteridoides were investigated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cd stress significantly (P < 0.05) decreased water content percentage (WC%), relative growth rate (RGR) and root activity in C. pteridoides, especially at highest Cd concentration (treatment CDT4). Significant (P < 0.05) drop of stress tolerance indices (STI) was noticed in C. pteridoides under treatment CDT4. Anatomical study of the Cd-treated C. pteridoides showed stomatal closure of leaves, reduction of diameter in xylem tracheids of stem and root, and decrease of intercellular spaces. Furthermore, ultrastructural alterations of leaf, stem, and root cells were evident with a damaged membrane system of chloroplast and mitochondria, disorganization of chloroplastic components, accumulation of large starch grains and plastoglobules, and formation of multivesicular bodies. The deposition of electron-dense material in the cell wall of root cells can be regarded as an important tolerance mechanism of C. pteridoides under Cd stress. Fourier transform infrared (FTIR) spectroscopy analysis of Cd-treated C. pteridoides biomass illustrated Cd-binding interaction with O-H, N-H, C-H, C≡C, CËO, PËO, -C-OH and CS functional groups of different metabolites.
ABSTRACT
Antimony (Sb) is considered as a priority toxic metalloid in the earth crust having no known biological function. The current study was carried out in a hydroponic experiment to study the accumulation of ecotoxic Sb in subcellular level, and to find out the ultrastructural damage caused by Sb in different vegetative parts of Trapa natans. Sb-induced structural and ultrastructural changes of T. natans were investigated using scanning electron microscope (SEM) and transmission electron microscope (TEM). Experimental plants were exposed to different Sb(III) treatments: SbT1 (1.5 µmol/L), SbT2 (40 µmol/L) and SbT3 (60 µmol/L). Calculated bioconcentration factor (BCF) and translocation factor (TF) showed that at higher concentration (SbT2, SbT3), T. natans is a potent phytoexcluder whereas it can translocate a substantial amount of Sb to the aerial parts at lower concentration (SbT1). SEM analysis revealed Sb-mediated structural changes in the size of stomatal aperture, intercellular spaces and vascular bundles of different vegetative tissues of T. natans. TEM results showed subcellular compartmentalization of Sb in vacuole and cell wall as electron dense deposition. This is considered as a part of strategy of T. natans to detoxify the deleterious effects under Sb stress conditions. Fourier transform infrared spectroscopy (FTIR) study of plant biomass revealed possible metabolites of T. natans which can bind Sb.
ABSTRACT
Hydroponic experiment was conducted to investigate the biochemical responses and accumulation behaviour of cadmium (Cd) in aquatic fern, Ceratopteris pteridoides, under four different levels of exposure. Plants were grown in 10 µM (CdT1), 20 µM (CdT2), 40 µM (CdT3) and 60 µM (CdT4) concentrations of Cd for 12 consecutive days and Cd accumulation in different plant parts, cell levels and growth medium was estimated. In C. pteridoides, Cd removal kinetics was best described by pseudo-second-order kinetic model. Increased accumulation of Cd in the plants was detected in a concentration dependent manner with maximum under 60 µM of Cd (CdT4) exposure (191.38 mg kg-1, 186.19 mg kg-1 and 1316.34 mg kg-1 in leaves, stems and roots, respectively). Cell wall of C. pteridoides is identified as crucial Cd storage site with the highest (28-69%) accumulation followed by organelles (14-44%) and soluble fraction (6-46%). Increased leaf proline, malondialdehyde (MDA) and protein content with significant reduction (P < 0.05) in chlorophyll concentration and upregulation of antioxidant enzymes catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) reveals the presence of Cd resistance mechanism in C. pteridoides. Calculated higher (>1) bioconcentration factor (BCF) and lower (<1) translocation factor (TF) values evinced the suitability of C. pteridoides in Cd phytostabilization rather than phytoextraction.
