[Optimizing the nursing process by new management instruments. The Northern Germany Nursing Research Network]. / Optimierung des Pflegeprozesses durch neue Steuerungsinstrumente: Der Pflegeforschungsverbund Nord.

The Northern Germany Nursing Research Network (Pflegeforschungsverbund Nord) has dedicated itself to developing management instruments intended to optimize the nursing process. It is coordinated by the administrative office of the Institute of Public Health and Nursing Research (Institut für Public Health und Pflegeforschung IPP, director: Prof. Dr. Stefan Görres), Department of "Interdisziplinäre Alterns- und Pflegeforschung", University of Bremen. Four partial projects are currently being worked on in two groups: Group A--"Deficient areas of nursing action": The projects belonging to this group are "Individual care-patterns of elderly people and their determinants" (University of Bremen) and "Mobility Restrictions in Nursing Homes-Multicentre Observational Study (MORIN)" (University of Hamburg). The purpose of these studies is to examine the relevance of quantitatively significant risk combinations from a nursing science perspective. Group B--"The generation and testing of nursing action management instruments": The projects in this area are "Nursing rounds as instruments of quality assurance in home-based care" (University of Applied Sciences, Bremen) and "Predicting the risk of falls, efficiency of standard scales in opposition to nursing assessment-Cluster-Randomised Controlled Trial (PROFESSION)" (University of Hamburg). These studies concentrate in a unique manner on the generation and testing of management/control instruments suitable for application to nursing action.

[High utilization of health care services by older adults]. / Alte Frauen und Männer mit starker Inanspruchnahme des Gesundheitswesens--Ein Beitrag zur altersspezifischen Versorgungsforschung.

Present discussions on health-care policy focus on fundamental restructuring, which will supposedly enable a more efficient provision of health care to patients with chronic ailments and also to elderly persons. Apart from increasing health care expenditure, the need for reform arises from demographically determined aging of the German population. A total of 54% of the 'GKV's' turnover in medical drugs is attributed to the health insurance agency's enrollees, who are aged 60 years and older, although they make up just 25% of total membership. Elderly men and women are therefore a significant group of health care users. In spite of its enormous political relevance, the provision of health care for the elderly has seen only marginal examination in Germany. This study on the "high utilization of health care services by older men and women" focuses on the examination of health care utilization by people aged 60 years and over. The following article will first introduce the study design. It will then review the literature on aging and health and on health care utilization by the elderly. The article goes on to present interim findings on the characteristics of the examined cohort and on ist consumption of medical drugs. The analysis is based on data regularly recorded by a North German health insurance agency. The examined cohort consists of 54% men and 46% women, whereby the average age is 68.3 years. The data analysis shows that 73,454 of the enrollees aged 60 years and older consumed 1,395,515 prescribed drugs in 2000. Each enrollee received an average of 19 medications annually; 5% of all those men and women examined received more than 58 medicines and were thus responsible for approximately a fifth of the volume in prescriptions. Of the drugs prescribed, 28.4% were for the treatment of cardio-vascular diseases, 13.9% for alimentary and metabolic disorders and 13.1% for the treatment of neurological diseases. Men and women aged 60 years and over are responsible for expenditure on medical drugs of 81,856,139 Deutsche Mark (41,926,298 Euro). On the average each enrollee generates costs of 1,114,00 Deutsche Mark (570 Euro), whereby every second one is responsible for an amount of less than 570 Deutsche Mark (291 Euro). A mere 5% of all enrollees is responsible for one third (32.1%) of the total drug expenditure. In closing the article will outline this research project's further course of action.

Gene expression profiles during the initial phase of salt stress in rice.

Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defense-related functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.

Quantification of active caspase 3 in apoptotic cells.

We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases. Capture on an ELISA plate with an antibody specific for caspase 3 makes the assay specific for caspase 3. Detection is with horseradish peroxidase (HRP)-conjugated streptavidin that binds to the biotinylated inhibitor covalently bound to caspase 3. Using the assay we detected 6.6 ng active caspase 3 per 10(6) apoptotic staurosporine-treated Jurkat cells. Specificity of the assay for caspase 3 was demonstrated by lack of signal with purified caspases 2, 7, 8, and 10 that were modified by a biotinylated inhibitor. Specificity was also demonstrated by lack of signal with apoptotic MCF-7 cells which do not express caspase 3. The ability to discriminate between active and latent caspase 3 was shown by Western blotting with HRP-streptavidin and anti-caspase 3. Although latent caspase 3 was captured it was not covalently modified with the biotinylated inhibitor. The basic principle of using a covalent inhibitor to identify active enzymes and an antibody to differentiate between enzymes with similar activities has potential for quantifying active members of many classes of enzymes.

CSF 5-HIAA, serum cortisol, and age differentially predict vegetative and cognitive symptoms in depression.

Prior studies have shown that both cerebrospinal fluid (CSF) concentrations of 5-hydroxyindolacetic acid (5-HIAA) and serum cortisol levels are related to overall symptom severity in depression. In the present study, 30 unmedicated inpatients meeting Research Diagnostic Criteria (RDC) criteria for depression participated in serum cortisol collection and a lumbar puncture for CSF. A multiple regression evaluated the ability of CSF 5-HIAA, serum cortisol, and age to predict cognitive and vegetative symptom clusters of the Hamilton Rating Scale for Depression. The multiple regression to predict the vegetative symptom cluster was highly significant overall (p = 0.002) and found that age and cortisol but not 5-HIAA predicted vegetative symptoms. The regression to predict the cognitive cluster narrowly missed overall significance (p = 0.06). Both CSF 5-HIAA and serum cortisol predicted cognitive symptoms and 5-HIAA predicted the cognitive cluster more strongly than cortisol. Age did not predict cognitive symptoms. The results suggest a dissociation between serum cortisol levels and CSF 5-HIAA in predicting vegetative and cognitive symptom clusters in depression.