ABSTRACT
OBJECTIVE: To report the functional results and morbidity after implantation of bulbar artificial urinary sphincter (AUS) in incontinent men with at least 5 years of follow-up. PATIENTS AND METHODS: The files of patients treated between 1984 and 2003 by setting up a SAU at bulbar location were reviewed. The following data were recorded: age, etiology of incontinence, intra-operative complications. To evaluate the results on continence, patients were seen at 1, 3, 6 and 12 months, and yearly thereafter. RESULTS: Overall, 159 men implanted with an AUS were included. The median age was 68.4 years and the median follow-up was 69 months. The etiology of incontinence was a prostate surgery in 83% of cases. The five-year rates of continence and satisfaction were 74.2% (n=118) and 92% (n=140), respectively. The five-year rates of infections and erosions were 4.4% and 9.4%, respectively. In case of previous radiotherapy, the rate of erosion at 5 years was 22.2%. The rate of atrophy and urethral mechanical failure at 5 years was 7.5% and 23.6%, respectively. The rate of reoperation at 5 years was 41.5%. CONCLUSION: Our results confirm that the AUS is still the standard treatment for male with urinary incontinence due to intrinsic sphincter deficiency. Minimally invasive therapies are being developed that could overcome the mechanical failures and offer an alternative in case of primary or even secondary failure of the AUS.
Subject(s)
Urinary Incontinence/surgery , Urinary Sphincter, Artificial , Aged , Follow-Up Studies , Humans , Male , Treatment OutcomeABSTRACT
El presente trabajo mide el impacto del agregado de flúor al agua potable en la reducción de caries dental en la población escolar de la localidad de Oberá. Misiones (Argentina) y evaluar la asociación entre los índices ceod y CPOD y variables cualitativas tales como el nivel socioeconómico, la higiene bucal y el acceso al agua potable florurada
ABSTRACT
El trabajo analiza las condiciones socioeconómicas considerando que estas no provocan una situación diferencial (estadisticamente significativa) en materia de caries cuando son evaluados al paso del estrato de niveles socieconómico más alto a los más bajos, aumentan los porcentajes de niños sin experiencia odontológica, con caries que sangran y lo que es más importante se incrementan el porcentaje de escolares que presentan una higiene dental calificada como mala y de los que no tienen acceso al agua potable. Estos resultados plantean la necesidad de mejorar la cobertura de agua potable de atención odontológica en los sectores de menores ingresos e insistir en la realización de campaña de concientización que enfaticen la importancia de una buena higiene dental como medio que contribuye a prevenir las caries
ABSTRACT
El trabajo analiza las condiciones socioeconómicas considerando que estas no provocan una situación diferencial (estadisticamente significativa) en materia de caries cuando son evaluados al paso del estrato de niveles socieconómico más alto a los más bajos, aumentan los porcentajes de niños sin experiencia odontológica, con caries que sangran y lo que es más importante se incrementan el porcentaje de escolares que presentan una higiene dental calificada como mala y de los que no tienen acceso al agua potable. Estos resultados plantean la necesidad de mejorar la cobertura de agua potable de atención odontológica en los sectores de menores ingresos e insistir en la realización de campaña de concientización que enfaticen la importancia de una buena higiene dental como medio que contribuye a prevenir las caries
Subject(s)
Dental CariesABSTRACT
El presente trabajo mide el impacto del agregado de flúor al agua potable en la reducción de caries dental en la población escolar de la localidad de Oberá. Misiones (Argentina) y evaluar la asociación entre los índices ceod y CPOD y variables cualitativas tales como el nivel socioeconómico, la higiene bucal y el acceso al agua potable florurada
Subject(s)
Dental Caries , Socioeconomic Factors , Quality of Life , Social ConditionsABSTRACT
Well-characterized pectin samples with a wide range of degrees of esterification (39-74%) were incubated with the solubilized pure alpha and gamma isoforms of pectinmethylesterase, from mung bean hypocotyl (Vigna radiata). Enzyme activity was determined at regular intervals along the deesterification pathway at pH 5.6 and pH 7.6. It has been demonstrated that the distribution of the carboxyl units along the pectin backbone controls the activity of the cell wall pectinmethylesterases to a much greater extent than the methylation degree, with a random distribution leading to the strongest activity. Polygalacturonic acid was shown to be a competitive inhibitor of the alpha isoform activity at pH 5.6 and to inhibit the gamma isoform activity at both pH 5.6 and pH 7.6. Under these conditions, the drop in enzyme activity was shown to be correlated to the formation of deesterified blocks of 19 +/- 1 galacturonic acid residues through simulations of the enzymatic digestion according to the mechanisms established previously (Catoire, L., Pierron, M., Morvan, C., Herve du Penhoat, C., and Goldberg, R. (1998) J. Biol. Chem. 273, 33150-33156). However, even in the absence of inhibition by the reaction product, activity dropped to negligible levels long before the substrate had been totally deesterified. Comparison of alpha and gamma isoform cDNAs suggests that the N-terminal region of catalytic domains might explain their subtle differences in activity revealed in this study. The role of pectinmethylesterase in the cell wall stiffening process along the growth gradient is discussed.
Subject(s)
Carboxylic Ester Hydrolases/metabolism , Fabaceae/enzymology , Isoenzymes/metabolism , Plants, Medicinal , Amino Acid Sequence , Base Sequence , Carboxylic Ester Hydrolases/chemistry , DNA Primers , Esterification , Hydrogen-Ion Concentration , Isoenzymes/chemistry , Kinetics , Molecular Sequence Data , Pectins/chemistry , Pectins/metabolism , Substrate SpecificityABSTRACT
Peptide maps and partial amino acid sequences of the 3 main pectinmethylesterases (PMEs) solubilized from mung bean hypocotyl cell walls demonstrated that these proteins were different isozymes originating from a small multigene family. A cDNA clone encoding the most alkaline PME (PE gamma) have been obtained by PCR using degenerate oligonucleotide primers. Combining the protein and nucleotide sequencing data, the complete amino acid sequence of PE gamma was determined. The nature protein is composed of 318 amino acids with a calculated Mtau of 34 677 and an estimated pI of 9.84 consistent with the values previously obtained by SDS-PAGE and IEF. It shares most of the conserved regions of previously known PMEs. Enzymatic activities of the three isoforms were differently affected by the presence of cations in the incubation medium but, in all cases, infra-optimal cation concentrations induced two opposite effects: a decrease in the Vmax and an increase in the affinity of the enzymes for their substrate. The presence of cations in the assay modulates both the number of enzyme molecules available to the demethylation reaction and the conformation of the pectin and, in turn, the affinity of the PMEs for their substrate.
Subject(s)
Carboxylic Ester Hydrolases/genetics , Cell Wall/enzymology , Fabaceae/genetics , Hypocotyl/enzymology , Isoenzymes/genetics , Plants, Medicinal , Amino Acid Sequence , Base Sequence , Carboxylic Ester Hydrolases/drug effects , Carboxylic Ester Hydrolases/metabolism , Cloning, Molecular , Fabaceae/enzymology , Hydrolysis , Isoenzymes/drug effects , Isoenzymes/metabolism , Kinetics , Molecular Sequence Data , Pectins/metabolism , Peptide Fragments/metabolism , Salts/pharmacology , Sequence Alignment , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
A cDNA clone encoding a pectinacetylesterase (PAE) was isolated from 3-day-old mung bean seedlings using PCR-based techniques. Degenerate oligonucleotide primers were designed according to the N-terminus and internal peptides from the purified PAE. The full-length clone of 1453 bp codes for a signal peptide of 24 amino acids and a mature protein of 375 amino acids. The Mr and the pI of the cDNA-deduced amino acid sequence agree with the values estimated for the purified enzyme. No significant sequence identity between the PAE and any known protein could be found in the databases. Northern analysis revealed developmentally regulated expression of the mRNA in mung been seedlings.
Subject(s)
Esterases/genetics , Fabaceae/enzymology , Pectins/metabolism , Plants, Medicinal , Amino Acid Sequence , Base Sequence , Blotting, Southern , Cloning, Molecular , DNA, Complementary , DNA, Plant , Esterases/metabolism , Gene Expression , Molecular Sequence Data , Plants , Polymerase Chain Reaction , RNA, Messenger , Sequence Homology, Amino AcidABSTRACT
An acetyl esterase was purified from cell walls isolated from mung bean hypocotyls. The purified enzyme had an apparent Mr of 43,300 and an apparent pI > 9. It rapidly deesterified triacetin and p-nitrophenylacetate and slowly released acetate from beet and flax pectins, the deesterification rate being increased by previous demethylation of the pectins. No significant peptide sequence identity between the acetyl esterase and any known protein could be found in protein data bases.
Subject(s)
Acetylesterase/metabolism , Fabaceae/enzymology , Hypocotyl/enzymology , Plant Proteins/metabolism , Plants, Medicinal , Acetylesterase/chemistry , Acetylesterase/isolation & purification , Amino Acid Sequence , Cell Wall/enzymology , Chromatography, High Pressure Liquid , Molecular Sequence Data , Peptide Fragments/chemistry , Peptide Fragments/isolation & purification , Peptide Mapping , Plant Proteins/chemistry , Plant Proteins/isolation & purificationABSTRACT
The nature and the action pattern of apoplastic pectinmethylesterase (PME) isoforms were investigated in mung bean [Vigna radiata (L.) Wilzeck] hypocotyls. Successive extractions of neutral and alkaline PME isoforms present in hypocotyl native cell walls (referred to as PE1, PE2, PE3, PE4, with increasingly basic isoelectric points) revealed that solubilization of PE1, PE2, and PE4 did not induce any significant decrease in the cell-wall-bound PME activity. The in vitro de-esterification occurring when isolated cell walls were incubated with pectin resulted, then, from the activity of PE3. In addition, pH control of PME activity was shown to be much stronger for enzymes bound to cell walls, in their native state or reintroduced after solubilization, than for enzymes in solution. Mature cell walls showed much more activity than young cell walls, and were relatively enriched in two acidic PME isoforms missing in young cell walls. One acidic PME was also detected in the extracellular fluid. The acidic and neutral isoforms that could be easily transferred from their binding sites to their substrate might be those involved in the demethylation process developing along the mung bean hypocotyl.
