[Biochemical changes in mouth fluid in people with acute emotional stress exposed to natural and increased radiation]. / Izmenenie biokhimicheskikh pokazatelei, kharakternykh dlia ostrogo émotsionalnogo stressa, v rotovoi zhidkosti u liudei v usloviiakh estestvennogo i povyshennogo fonov radioaktivnosti.

Acute emotional stress in people is followed by changes in oral fluid composition (sialic acid, components of proteolytic system, lipid of peroxidation products). The possibility to use these indices for estimation of the psychoemotional stress in people is based.

[A comparative study of the expediency of using different chemical methods for determining protein in allergen preparations]. / Sravnitel'noe izuchenie tselesoobraznosti primeneniia razlichnykh khimicheskikh metodov dlia opredeleniia belka v preparatakh allergenov.

Parallel use of some known chemical procedures for measurements of protein in commercial forms of allergens revealed considerable variations in the results, which was due to the nature of the methods used and to admixtures of different substances in the preparations under study. The conclusion on the necessity of using at least two methods of protein determination for the standardization of allergens was made. Preference was given to Bradford's method. As the standardization of allergens manufactured in the USSR is carried out on the basis of the quantitative determination of protein (in PNU), this problem is of not only theoretical, but also practical importance.

[The immunochemical characteristics of allergens]. / Immunokhimicheskaia kharakteristika allergenov.

Modern approaches to the standardization of allergens require the use of not only biological methods, but also a wide range of physicochemical and immunological ones. To obtain Soviet standard samples, the optimum spectrum of methods for their characterization, evaluation and comparison with international standard samples should be definitely selected. After the preliminary study of a number of allergens by different immunochemical methods a set of such methods, including different kinds of electrophoresis, isoelectrofocusing, immunoblotting, etc., are proposed, these methods being considered obligatory for obtaining Soviet standard samples of allergens.

[Evaluation of the level of antibodies to purified meningococcal antigens]. / Otsenka urovnia antitel k ochishchennym antigenam meningokokka.

Group B meningococcal antigens, such as polysaccharide, lipopolysaccharide, protein preparation, as well as sonicates obtained from meningococcal cells, groups A, B and C, have been isolated. On the basis of these preparations the parameters of an enzyme immunoassay system for the detection of antibodies to individual meningococcal antigens have been established, and the specificity of the system and the possibility of using it for the evaluation of the level of antibodies to meningococci in human sera have been studied.

[Possible serodiagnosis of pertussis infection by an immunoenzyme method]. / O vozmozhnosti serodiagnostiki kokliushnoi infektsii immunofermentnym metodom.

The sera of children and adults with a history of pertussis, as well as the sera of children immunized in three injections, have been studied in the enzyme immunoassay. The levels of antibodies to Bordetella pertussis protein and lipopolysaccharide, and to disintegrated B. pertussis cells have been determined; a serum titer of 1:1,600 and higher is considered as a criterion for the serological diagnosis of pertussis.

[Creation of the library of Bordetella pertussis genome clones and detection of clones containing genes for the synthesis of antigenic determinants of the whooping cough microbe]. / Sozdanie klonoteki genoma Bordetella pertussis i obnaruzhenie v nei klonov, soderzhashchikh geny sinteza antigennykh determinant kokliushnogo mikroba.

Escherichia coli clones containing hybrid phasmids with the inserts of B. pertussis DNA were obtained with the use of a phasmid vector. The total amount of the clones thus obtained was 97,000, which considerably exceeded the volume of the clone library necessary for the detection of individual genes with probability approximating 1. The hybrid plasmids were shown to contain 6-19 kilobases. The screening of the clone library was carried out by means of the enzyme immunoassay (EIA). The assay was aimed at detecting clones containing the genes of the subunits of B. pertussis lymphocytosis-stimulating factor (LSF). The EIA techniques used in this investigation were based on the capacity of LSF for binding with fetuin. Six clones giving positive response were detected. These data suggest the presence and expression of the genes controlling the synthesis of the antigenic determinants of LSF in E. coli cells.

[Immune response to various antigenic preparations of Bordetella pertussis in whooping cough patients and convalescents studied by immunoenzyme analysis]. / Izuchenie immunnogo otveta na razlichnye antigennye preparaty kokliushnogo mikroba u bol'nykh kokliushem i rekonvalestsentov s pomoshch'iu immunofermentnogo analiza.

The content of antibodies to B. pertussis disintegrated cells, protein fraction and lipopolysaccharide in the blood of patients and convalescents has been studied. The study has revealed that in the blood sera of whooping cough patients the titers of antibodies to lipopolysaccharide considerably exceed the titers of antibodies to other antigenic fractions. The titers of IgM to lipopolysaccharide have been shown to exceed the titers of IgM to other fractions 1.5-2 times.

[Antipertussis immunity studied by immunoenzyme analysis]. / Izuchenie protivokokliushnogo immuniteta s pomoshch'iu immunofermentnogo analiza.

The levels of antibodies to disintegrated Bordetella pertussis and its individual fractions (protein and polysaccharide) in children immunized with different batches of adsorbed DPT vaccine have been determined with the use of EIA techniques. The background level of antibodies in the control groups has been determined, and in immunized children the levels of antibodies to disintegrated B. pertussis and its protein fraction have been shown to considerably exceed the levels of antibodies to lipopolysaccharide.