[A comparative study of the biological properties of pyelonephritogenic Escherichia coli isolated at different times in the infectious process]. / Sravnitel'noe issledovanie biologicheskikh svoistv pielonefritogennykh Escherichia coli, vydelennykh v razlichnye sroki infektsionnogo protsessa.

The comparative study of the biological properties of E. coli cultures, isolated from the urine of 7 patients two times during the first 11 days from the beginning of clinical manifestations of the exacerbation of chronic pyelonephritis, was conducted. In most cases the strains obtained as the result of the inoculations of the first and second urine samples belonged to the same serological and enzymatic variants. Still bacteria isolated in the second investigation, in contrast to E. coli obtained by the earlier inoculation of urine samples, often had no hemagglutinins and showed low adhesive capacity with respect to uroepithelium. Only in one out of 4 patient E. coli with antigen K1+ could be detected not only after the first inoculation, but also after the second one. In 4 patients E. coli cultures obtained as the result of the second isolation of these bacteria had lower content of sialic acid. Besides, differences in the sensitivity of E. coli strains isolated from the same patients in the course of the infectious process to the action of nonspecific protection factors of the body were established. The results obtained in the course of this study give more precise understanding of the existing conception of the pathogenesis of pyelonephritis.

[Microflora in colonic cancer complicated by an inflammatory process]. / Mikroflora pri rake obodochnoi kishki, oslozhnennom vospalitel'nym protsessom.

The microbiological investigation was performed in 53 patients with carcinoma of the colon, 32 of them having inflammatory complications of the tumor. High incidence of the infected state of the tumor surface, infected regional lymph nodes and regional venous blood was noted especially by enterobacteria. The bacteria isolated were found to be highly resistant to most widely used antibiotics. For the antibiotics to have a bacteriostatic or bactericidal effect, they must be used in great doses which must be often higher than therapeutically admitted ones.

[Effect of plasmids for multiple antibiotic resistance on the change in Salmonella typhimurium phagovar]. / Vliianie plazmid mnozhestvennoi ustoichivosti k antibiotikam na izmenenie fagovara Salmonella typhimurium.

The data on the influence of acquired plasmid resistance to antibiotics on S. typhimurium phagovar. Plasmid R was transferred from S. typhimurium strain, isolated from the focus of hospital salmonellosis and resistant to the lytic action of phages, to Escherichia coli K12 and then to antibiotic-sensitive S. typhimurium strains of different phagovars, isolated from patients with alimentary toxicoinfections. The influence of plasmid R on the phagovar of recipient strains, most pronounced in strains of phagovar I, was revealed. The transconjugates of this phagovar considerably changed sensitivity to phages and in some cases acquired resistance to the lytic action of typing phages, thus becoming identical in this feature to the donor of the plasmid.

[Formation of donor strains of enteropathogenic Escherichia, serogroups O112ac, O129, O143, and Sh. boydii 8 using R-plasmids]. / Formirovanie donorskikh shtammov énteropatogennykh ésherikhii serogrupp O112ac, O129, O143 i Sh. boydii 8 s pomoshch'iu R-plazmid.

The possibility of the formation of enteropathogenic Escherichia and Shigella donor strains by means of R-plasmids RP4 and RPI-2 with thermosensitive replication was studied. The donor strains of E. coli, serogroups O112ac, O129, O143, and Sh. boydii 8 were thus obtained; these strains had the above-mentioned plasmids integrated into their chromosome, and in the process of conjugation they transferred plasmid and chromosomal genes to E. coli, K12 and O129, with the effectiveness 10(5)--10(8). Such donor strains may probably be used for studying the mechanisms of the formation of new enteropathogenic Escherichia and Shigella biovars and for their genetic analysis.

[Phage conversion of the antigens in Escherichiae and Shigellae. 2. Conversion of the somatic antigen in Shigella flexneri with the moderate E. coli 0129 phage]. / Fagovaia konversiia antigenov u Esherikhii i Shigell. Soobshchenie II. Konversiia somaticheskogo antigena u Sh. flexneri umerennym fagom E. coli 0129.

The authors studied the converting activity of the moderate EF5 phage isolated from the lysogenic E. coli 0129 strain. It was shown that this phage converted the O-antigen with the detection of the type antigen V in the strains of Sh. flexneri of the serological type la and y-variant. The converted cultures contained the type antigen V and were identical by the antigenic properties to one another and the Sh. flexneri of the serological type 5 and E. coli 0129. A conclusion was drawn that phages converting the antigens of Sh. flexneri could be encountered in escherichia and could modify the antigens in Sh. flexneri and escherichia possessing the antigenic factor 3,4.

[Phage conversion of escherichia and shigella antigens. I. Phage conversion of type I antigen of Sh. flexneri in entrophatogenic E. coli O129]. / Fagovaia konversiia antigenovu esherikhii i shigell. Soobshchenie I. Fagovaia konversiia tipovogo

The authors realized conversion of type I Sh. flexneri in enteropathogenic E. coli O129 with converting moderate phage phi I Sh. flexneri. Phage phi I lysogenized 7.3--42.7% of the cells of antigenic E. coli variant O129 which lost type V antigen; conversion of the type I antigen of Sh. flexneri was revealed in the agglutination and adsorption of agglutinins tests. As a result, E. coli strain was obtained with the O-antigen identical to the O-antigen of Sh. flexneri Ia.

[Independent integration of genes controlling the invasive properties and streptomycin resistance of enteropathogenic escherichiae 0124]. / Nezavisimaia integratsiia genov, kontroliruiushchikh invazivnye svoistva i ustoichivost' k streptomitsinu u énteropatogennykh ésherikhii 0124

Recombinations which lost their invasive properties were obtained by crossing E. coli K12Hfr AB313 with entropathogenic escherichia belonging to serological group 0124. The loss of invasive properties by these recombinations was not connected with the acquisition of genes controlling the streptomycin resistance.

[Phagocytosis of enteropathogenic escherichiae of serogroup 0124:K72(B17)]. / Fagotsitoz énteropatogennykh ésherikhii serogruppy 0124:K72(B17)

Experiments with phagocytosis of E. coli O124 in the macrophage tissue culture demonstrated a different phagocytability of the S- and R-forms of these bacteria determined by their concentration in the medium. The ingestive and digestive capacity depended on the bacterial virulence. Since R-forms of E. coli O124 lost their virulent properties and macrophage resistance it can be considered that the structure of their lipopolysaccharides served as the chemical substrate responsible for their different phagocytability.

[Biochemical and genetic characteristics of enteropathogenic Escherichia coli and shigellae. IV. An electron microscopic study of pathogenic escherichia of serologic group 0124 and their recombinants]. / Biokhimicheskaia i geneticheskaia kharakteristika énteropatogennykh kishechnykh palochek i shigell. Soobshchenie IV. Elektronno-mikroskopicheskoe issledovanie patogennykh ésherikhii serologicheskoi gruppy 0124 i ikh rekombinantov

Electron microscopic study of parental strains E. coli K12, E. coli 0124 : K72 (B17 and of recombinations demonstrated that their populations were nonhomogeneous and, along with the common ones, contained maxi- and mini-cells. By the character of flagellae and fimbria inheritance recombinations were distributed into two groups: similar to the donor and similar to the recipient. The data of electron microscopic determination of the flagellae and fimbria failed to coincide with the data on mobility and capacity to agglutinate erythrocytes in all the strains under study; this pointed to the nonhomogeneity of bacterial population in respect to these signs. There were revealed recombinations of E. coli O124 in which the majority of the population cells had flagellae, but proved to be immobile; this indicated a possibility of independent transfer of genes controlling the morphogenesis and the function of the flagellae to the E. coli O124 recombinations.