[Immunocorrection with pentaglobin in wound infection and burn disease]. / Immunokorrektsiia pentaglobinom pri ranevoi infektsii i ozhogovoi bolezni.

Patients with wound infections and extended burns were treated with pentaglobin (Biotest-Pharma), a serum preparation containing high concentrations of immunoglobulin M. The use of the preparation at early stages after surgical operations in the patients with wound infections or at the beginning of active surgical treatment of the patients with burns decreased the terms of the body temperature normalization as well as normalization of the immunological, hematological and biochemical indices. The most pronounced efficacy of pentaglobin was observed in the patients with severe microbial toxemia. A scheme for pathogenetic immunocorrection of wound infections and burns was developed. It is based on simultaneous recording of intensity of immunological responses in peripheral blood and intensity of protective reactions in wound tissues.

[Sandoglobulin immunocorrection in trauma]. / Immunokorrektsiia sandoglobulinom pri travme.

Twenty one patients with the long-term compression syndrome (LCS) and 12 patients with burns treated with sandoglobulin in combination with antibacterial therapy were followed up. The control groups included 14 and 18 patients, respectively. All the patients had wound infections. Increased or lowered respiratory burst of peripheral blood neutrophils and lowered contents of active T-lymphocytes were detected in the majority of the patients. The patients had also an increased respiratory burst of tissue homogenate in the primary focus. Sandoglobulin decreased the periods of normalization of the immunological indices, body temperature and leukogram shifts to the right. The most pronounced effect of the drug was recorded before radical operations, i.e. in the presence of acute microbial toxemia or in patients with severe and extended burns. The procedure of immunological monitoring developed by the authors rapidly estimates the indications to the use of sandoglobulin alone or in combination with other immunomodulators.

[Lymphocytes with antigen receptors in drug allergy]. / Limfotsity s retseptorami dlia antigena pri lekarstvennoi allergii.

The data on the possibility of using the rosette-formation tests for the diagnosis of drug allergy are presented. Tests based on changes in the levels of activated T- and B-lymphocytes after their incubation with allergenic drugs have proved to be low informative. The test found to be highly informative is the antigen-specific rosette-formation test based on the detection of lymphocytes, capable of binding allogeneic erythrocytes loaded with antibiotics causing allergy in patients, in the peripheral blood. This test may be of importance not only in diagnosis, but also for prognosis, as it permits the detection of sensitization to a drug before the clinical manifestations of allergy.

[Circulating immune complexes and heterophilic hemolysins in burn injury]. / Tsirkuliruiushchie immunnye kompleksy i geterofil'nye gemoliziny pri ozhogovoi bolezni.

A reverse correlations was disclosed between high level of circulating immune complexes (CIC) and low titers of heterophilic hemolysins (HH) on the one hand and burn disease gravity, on the other one. The decrease in HH activity in the serum of some patients depended not only on the genuine inhibition of lymphocyte stimulation but also on serum anti-complementary characteristics and/or on a low complement level. The devised scheme for determining the CIC and HH titer levels can be used for the evaluation of the efficacy of detoxifying therapy and burn toxemia gravity.

[Oxygen demand of an Actinomyces rimosus culture dependent on the composition of the medium]. / Potrebnost' kul'tury Actinomyces rimosus v kislorode v zavisimosti ot sostava sredy

Consumption of oxygen by Act. rimosus depending on the medium composition was studied. It was shown that the level of dissolved oxygen in the fermentation broth decreased as the level of carbohydrates in the fermentor increased from 6 to 7.5 per cent. Under such conditions oxytetracycline biosynthesis appeared to be limited by oxygen and the maximum level of the antibiotic was not attained. A 2-fold increase in the concentration of the medium components resulted in an almost 2-fold increase of the biomass, while the potency level increased only by 54 per cent. The oxygen deficiency lowered the mycelium productivity with respect to the antibiotic biosynthesis to a greater extent that the rate of the culture growth.