ABSTRACT
There is growing interest on the impact of diet on depressive disorders. However, there are limited data on the association between dietary patterns and depression symptoms among Iranian adolescents. The aim of this study was to evaluate the association between dietary patterns and depression score among Iranian adolescent girls. Data were obtained from 750 adolescent girls, who were recruited from several schools using a random cluster sampling. Dietary data were collected using a valid and reliable food frequency questionnaire (FFQ). Beck's depression inventory (BDI-II) was used to assess depression severity score. The prevalence of girls diagnosed with depression was 29.1%. Three major dietary patterns were identified in the study based on factor analysis: "Healthy", "Traditional" and "Western". The multivariate-adjusted odds ratio of having depressive symptoms was 0.55 (95% confidence intervals, 0.34-0.89) for the highest versus lowest quartile of the healthy dietary pattern score. However, there were no significant associations between the Traditional and Western dietary patterns and depression symptoms. Our results indicate that adherence to a Healthy dietary pattern characterized by high intakes of fruits, vegetables, fish and dairy products is associated with a lower probability of having depressive symptoms among Iranian adolescent girls.
Subject(s)
Depressive Disorder/epidemiology , Depressive Disorder/psychology , Diet, Healthy/psychology , Adolescent , Child , Cross-Sectional Studies , Depressive Disorder/diet therapy , Diet, Healthy/trends , Diet, Western/adverse effects , Diet, Western/psychology , Female , Humans , Iran/epidemiology , Prevalence , Psychiatric Status Rating Scales , Surveys and QuestionnairesABSTRACT
Here we explored the antitumor-activity of novel-formulated-form of curcumin (phytosomal-encapsulated-curcumin) or in combination with 5-FU in breast cancer. The antiproliferative activity was assessed in 2D and 3-dimensional cell-culture-model. The migratory-behaviors of the cells were determined by migration assay. The expression levels of CyclinD1,GSK3a/b, P-AMPK, MMP9, and E-cadherin were studied by qRT-PCR and/or Western blotting. The anti-inflammatory of nano-curcumin was assessed, while antioxidant activity was evaluated by malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and total thiols (T-SH). To understand dynamic behavior of genes, we reconstructed a Boolean network, while the robustness of this model was evaluated by Hamming distance. phytosomal-curcumin suppressed cell-growth followed by tumor-shrinkage in 3D model through perturbation of AMP-activated protein kinase. Curcumin reduced the invasiveness of MCF-7 through perturbation of E-cadherin. Moreover, phytosomal-curcumin inhibited the tumor growth in xerograph model. Histological staining of tumor tissues revealed vascular disruption and RBC extravasation, necrosis, tumor stroma, and inflammation. Co-treatment of curcumin and 5-FU reduced the lipid-peroxidation and increased MDA/SOD level. Of note, curcumin reduced cyclinD-expression in breast cancer cell treated with thrombin, and activates AMPK in a time-dependent manner. Also suppression of AMPK abrogated inhibitory effect of phytosomal-curcumin on thrombin-induced cyclin D1 over-expression, suggesting that AMPK is essential for anti-proliferative effect of this agent in breast cancer. Our finding demonstrated that phytosomal-curcumin antagonizes cell growth and migration, induced by thrombin through AMP-Kinase in breast cancer, supporting further-investigations on the therapeutic potential of this novel anticancer agent in treatment of breast cancer.
Subject(s)
AMP-Activated Protein Kinases/metabolism , Breast Neoplasms/drug therapy , Cell Movement/drug effects , Cell Proliferation/drug effects , Curcumin/pharmacology , Gene Expression Regulation, Neoplastic/drug effects , Thrombin/adverse effects , Animals , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Breast Neoplasms/chemically induced , Breast Neoplasms/enzymology , Breast Neoplasms/pathology , Drug Compounding , Female , Hemostatics/adverse effects , Humans , Mice , Mice, Inbred BALB C , Mice, Nude , Tumor Cells, Cultured , Xenograft Model Antitumor AssaysABSTRACT
Aberrant activation of the HGF/c-Met signalling pathway is reported to be associated with cell proliferation, progression, and metastasis features of several tumor types, including cervical cancer, suggesting that it may be of potential value as a novel therapeutic target. Furthermore, HPV-positive patients had a higher serum level of HGF or c-Met protein, compared with HPV-negative patients. c-Met or HGF overexpression in lesions of cervical cancer is reported to be related to a poorer prognosis, and hence this may be of value as a prognostic and predictive biomarker. Several approaches have been developed for targeting HGF and/or c-Met. One of these is crizotinib (a dual c-Met/ALK inhibitor). This has been approved by FDA for the treatment of lung-cancer. Further investigations are required to evaluate and optimize the use of c-Met inhibitors in cervical cancer or parallel targeting signalling pathway associated/activated via MET/HGF pathway. The main aim of current review was to give an overview of the potential of the c-Met/HGF pathway as a prognostic, or predictive biomarker in cervical cancer.
