ABSTRACT
There is an urgent need to understand severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-host interactions involved in virus spread and pathogenesis, which might contribute to the identification of new therapeutic targets. In this study, we investigated the presence of SARS-CoV-2 in postmortem lung, kidney, and liver samples of patients who died with coronavirus disease (COVID-19) and its relationship with host factors involved in virus spread and pathogenesis, using microscopy-based methods. The cases analyzed showed advanced stages of diffuse acute alveolar damage and fibrosis. We identified the SARS-CoV-2 nucleocapsid (NC) in a variety of cells, colocalizing with mitochondrial proteins, lipid droplets (LDs), and key host proteins that have been implicated in inflammation, tissue repair, and the SARS-CoV-2 life cycle (vimentin, NLRP3, fibronectin, LC3B, DDX3X, and PPARγ), pointing to vimentin and LDs as platforms involved not only in the viral life cycle but also in inflammation and pathogenesis. SARS-CoV-2 isolated from a patient´s nasal swab was grown in cell culture and used to infect hamsters. Target cells identified in human tissue samples included lung epithelial and endothelial cells; lipogenic fibroblast-like cells (FLCs) showing features of lipofibroblasts such as activated PPARγ signaling and LDs; lung FLCs expressing fibronectin and vimentin and macrophages, both with evidence of NLRP3- and IL1ß-induced responses; regulatory cells expressing immune-checkpoint proteins involved in lung repair responses and contributing to inflammatory responses in the lung; CD34+ liver endothelial cells and hepatocytes expressing vimentin; renal interstitial cells; and the juxtaglomerular apparatus. This suggests that SARS-CoV-2 may directly interfere with critical lung, renal, and liver functions involved in COVID-19-pathogenesis.
Subject(s)
COVID-19 , Humans , COVID-19/pathology , Fibronectins , Vimentin , SARS-CoV-2 , Endothelial Cells , NLR Family, Pyrin Domain-Containing 3 Protein , PPAR gamma , Lung , Inflammation/pathology , Kidney , LiverABSTRACT
We herein report, by using confocal immunofluorescence, the colocalization of the SARS-CoV-2 nucleocapsid within neurons, astrocytes, oligodendrocytes and microglia in three deceased COVID-19 cases, of between 78 and 85 years of age at death. The viral nucleocapsid was detected together with its ACE2 cell entry receptor, as well as the NLRP3 inflammasome in cerebral cortical tissues. It is noteworthy that NLRP3 was colocalized with CD68 + macrophages in the brain and lung of the deceased, suggesting the critical role of this type of inflammasome in SARS-CoV-2 lesions of the nervous system/lungs and supporting its potential role as a therapeutic target.
Subject(s)
Brain/virology , COVID-19/virology , Inflammasomes/immunology , Microglia/virology , NLR Family, Pyrin Domain-Containing 3 Protein/immunology , SARS-CoV-2/pathogenicity , Aged , Aged, 80 and over , Astrocytes/virology , Autopsy , Brain/immunology , Brain/pathology , COVID-19/immunology , COVID-19/pathology , Female , Humans , Male , Microglia/immunology , Neurons/virology , Nucleocapsid , Oligodendroglia/virologyABSTRACT
Se informa el caso de una paciente con histiocitosis X tipo Hands-Schuller, que afecta huesos y partes blandas. Corroborándose el diagnóstico mediante el estudio histopatológico de las lesiones observadas en la región perianal y en la vulva (AU)
Subject(s)
Adult , Humans , Female , Histiocytosis, Langerhans-CellABSTRACT
Se plantean la metodología, resultados, discusión y conclusiones del estudio experimental de bagazosis, para precisar la importancia de la microflora y del dióxido de silício en el proceso patológico pulmonar y aclarar las supuestas propiedades alergénicas del polvo de bagazo. Se utilizaron 46 curieles machos, se sometieron al análisis estadístico por método paramétrico y no paramétrico, presentándose las cifras promedios con sus errores estándares(AU)
Subject(s)
PneumoconiosisABSTRACT
Se plantean la metodología, resultados, discusión y conclusiones del estudio experimental de bagazosis, para precisar la importacian de la microflora y del dióxido de silício en el proceso patológico pulmonar, y aclarar las supuestas propiedades alergénicas del polvo de bagazo. Se utilizaron 46 curieles machos. Los resultados se sometieron al análisis estadístico por métodos para métricos y no paramétricos, presentándose las cifras promedios con sus errores estándares(AU)
Subject(s)
Humans , Respiratory Tract Diseases/microbiology , Fungi , Occupational Health , Fungicides, Industrial/analysisABSTRACT
Hemos presentado un caso de amiloidosis primaria o atípica con predominio de macroglosia. El diagnóstico se hizo en vida por biopsia de lengua y encía. No pudimos comprobar lesiones amiloidóticas de otras vísceras.Se descartó la posibilidad de mieloma múltiple(AU)
Subject(s)
Amyloidosis/diagnosis , Amyloidosis/complications , Macroglossia/complicationsABSTRACT
Se presentan cuantro casos de imágenes lacunares redondeadas gástricas, comprobándose en el acto quirúrgico lo siguiente: en el primer caso un pólipo fibroide inflamatorio, en los casos 2 y 3 fitobezoares concomitando con lesión ulcerosa estenosamente y en el caso no. 4 un quiste enterogénico.Se hace una revisión de las causas más frecuentes de estas imágenes, insistiéndose en el diagnóstico diferencial de las mismas es bastante difícil desde el punto de vista radiológico, siendo el estudio anatomopatológico el único definitivo(AU)
