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1.
Mol Neurobiol ; 55(10): 8124-8153, 2018 Oct.
Article in English | MEDLINE | ID: mdl-29508283

ABSTRACT

The NH2tau 26-44 aa (i.e., NH2htau) is the minimal biologically active moiety of longer 20-22-kDa NH2-truncated form of human tau-a neurotoxic fragment mapping between 26 and 230 amino acids of full-length protein (htau40)-which is detectable in presynaptic terminals and peripheral CSF from patients suffering from AD and other non-AD neurodegenerative diseases. Nevertheless, whether its exogenous administration in healthy nontransgenic mice is able to elicit a neuropathological phenotype resembling human tauopathies has not been yet investigated. We explored the in vivo effects evoked by subchronic intracerebroventricular (i.c.v.) infusion of NH2htau or its reverse counterpart into two lines of young (2-month-old) wild-type mice (C57BL/6 and B6SJL). Six days after its accumulation into hippocampal parenchyma, significant impairment in memory/learning performance was detected in NH2htau-treated group in association with reduced synaptic connectivity and neuroinflammatory response. Compromised short-term plasticity in paired-pulse facilitation paradigm (PPF) was detected in the CA3/CA1 synapses from NH2htau-impaired animals along with downregulation in calcineurin (CaN)-stimulated pCREB/c-Fos pathway(s). Importantly, these behavioral, synaptotoxic, and neuropathological effects were independent from the genetic background, occurred prior to frank neuronal loss, and were specific because no alterations were detected in the control group infused with its reverse counterpart. Finally, a 2.0-kDa peptide which biochemically and immunologically resembles the injected NH2htau was endogenously detected in vivo, being present in hippocampal synaptosomal preparations from AD subjects. Given that the identification of the neurotoxic tau species is mandatory to develop a more effective tau-based immunological approach, our evidence can have important translational implications for cure of human tauopathies.


Subject(s)
Alzheimer Disease/metabolism , Alzheimer Disease/pathology , Immunotherapy , tau Proteins/chemistry , tau Proteins/metabolism , Alzheimer Disease/complications , Alzheimer Disease/physiopathology , Animals , Behavior, Animal , Cognition , Cyclic AMP Response Element-Binding Protein/metabolism , Gliosis/complications , Gliosis/pathology , Gliosis/physiopathology , Hippocampus/pathology , Hippocampus/physiopathology , Humans , Inflammation/pathology , Male , Memory , Memory Consolidation , Mice, Inbred C57BL , Neuronal Plasticity , Neuropathology , Neurotransmitter Agents/metabolism , Peptides/metabolism , Proto-Oncogene Proteins c-fos/metabolism , Solubility , Synapses/metabolism , Synaptosomes/metabolism , Task Performance and Analysis
2.
Hum Mol Genet ; 24(11): 3058-81, 2015 Jun 01.
Article in English | MEDLINE | ID: mdl-25687137

ABSTRACT

Disarrangement in functions and quality control of mitochondria at synapses are early events in Alzheimer's disease (AD) pathobiology. We reported that a 20-22 kDa NH2-tau fragment mapping between 26 and 230 amino acids of the longest human tau isoform (aka NH2htau): (i) is detectable in cellular and animal AD models, as well in synaptic mitochondria and cerebrospinal fluids (CSF) from human AD subjects; (ii) is neurotoxic in primary hippocampal neurons; (iii) compromises the mitochondrial biology both directly, by inhibiting the ANT-1-dependent ADP/ATP exchange, and indirectly, by impairing their selective autophagic clearance (mitophagy). Here, we show that the extensive Parkin-dependent turnover of mitochondria occurring in NH2htau-expressing post-mitotic neurons plays a pro-death role and that UCHL-1, the cytosolic Ubiquitin-C-terminal hydrolase L1 which directs the physiological remodeling of synapses by controlling ubiquitin homeostasis, critically contributes to mitochondrial and synaptic failure in this in vitro AD model. Pharmacological or genetic suppression of improper mitophagy, either by inhibition of mitochondrial targeting to autophagosomes or by shRNA-mediated silencing of Parkin or UCHL-1 gene expression, restores synaptic and mitochondrial content providing partial but significant protection against the NH2htau-induced neuronal death. Moreover, in mitochondria from human AD synapses, the endogenous NH2htau is stably associated with Parkin and with UCHL-1. Taken together, our studies show a causative link between the excessive mitochondrial turnover and the NH2htau-induced in vitro neuronal death, suggesting that pathogenetic tau truncation may contribute to synaptic deterioration in AD by aberrant recruitment of Parkin and UCHL-1 to mitochondria making them more prone to detrimental autophagic clearance.


Subject(s)
Alzheimer Disease/genetics , Neurons/metabolism , Ubiquitin Thiolesterase/metabolism , Ubiquitin-Protein Ligases/metabolism , tau Proteins/genetics , Alzheimer Disease/metabolism , Alzheimer Disease/pathology , Animals , HeLa Cells , Humans , Mice, Inbred C57BL , Mice, Transgenic , Mitochondrial Proteins/metabolism , Mitophagy , Neurons/physiology , Protein Transport , Rats, Wistar , tau Proteins/physiology
3.
Eur J Neurol ; 16(1): 121-6, 2009 Jan.
Article in English | MEDLINE | ID: mdl-19087158

ABSTRACT

BACKGROUND AND PURPOSE: Autosomal Recessive Hereditary Spastic Paraplegia with Thin Corpus Callosum (AR-HSPTCC) is a clinically and genetically heterogeneous complicated form of spastic paraplegia. Two AR-HSPTCC loci have been assigned to chromosome 15q13-15 (SPG11) and chromosome 8p12-p11.21 respectively. Mutations in the SPG11 gene, encoding the spatacsin protein, have been found in the majority of SPG11 families. In this study, involvement of the SPG11 or 8p12-p11.21 loci was investigated in five Italian families, of which four consanguineous. METHODS: Families were tested for linkage to the SPG11 or 8p12-p11.21 loci and the SPG11 gene was screened in all the affected individuals. RESULTS: Linkage was excluded in the four consanguineous families. In the only SPG11-linked family the same homozygous haplotype 4.2 cM across the SPG11 locus was shared by all the three affected siblings. A novel c.2608A>G mutation predicted to affect the splicing was found in exon 14 of the SPG11 gene. DISCUSSION: This collection of families contributes to highlight the intra and inter locus heterogeneity in AR-HSPTCC, already remarked in previous reports. In particular, it confirms heterogeneity amongst Italian families and reports a new mutation predicted to affect splicing in the spatacsin gene.


Subject(s)
Agenesis of Corpus Callosum/genetics , Genetic Predisposition to Disease/genetics , Mutation/genetics , Nervous System Malformations/genetics , Proteins/genetics , Spastic Paraplegia, Hereditary/genetics , Adolescent , Adult , Agenesis of Corpus Callosum/metabolism , Agenesis of Corpus Callosum/physiopathology , Female , Humans , Male , Nervous System Malformations/metabolism , Nervous System Malformations/physiopathology , Pedigree , Proteins/metabolism , Spastic Paraplegia, Hereditary/metabolism , Spastic Paraplegia, Hereditary/physiopathology , Young Adult
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