Morphological examination of young adults related to obesity / Examen morfológico de adultos jóvenes relacionado con la obesidad

SUMMARY: This study presents a cross-board comparison of the morphological characteristic of students of the Hungarian Language teacher training faculty in Subotica, Serbia based on their nutritional status estimate of their nourishment state. The sample was composed by 146 young adults from all four study years of the faculty, ranging from age 19 to 23. The following anthropometric measurements were carried out: body weight, height, skin fold thickness (skin folds measured at the scapula, triceps, biceps, ilium, hip, thigh and abdomen) and circumferences (waist, hip). The authors calculated and analyzed the data, including: the BMI (body mass index) with the formula, skin fold thickness and with 4-Site Skin fold Equation and waist hip ratio. Descriptive statistic was used to describe the morphological characteristics. Independent T-test and ANOVA analysis was performed to compare the students according to sex and age. The main results of the present study are: (1) no significant difference is found between the age groups in the case of either height or body weight, not for the young men or the young women in the study; (2) the differences between height and weight in the case of the male and female as characteristic for this age group can be observed; (3) the female students are more often found to be overweight or obese than the male students; (4) the majority of participant students can be classified into the normal nutritional status' category, which holds true for both the young men and women of the study; (5) the fat percentages calculated based on skin fold values show that in terms of weight, the majority of the female students fall under the 'acceptable' category, while the majority of the young men are classed as 'thin'; (6) in the case of abdominal overweight 20.0 % of men and 19.48 % of women belong to the category 'obese'. This information about the students of the teacher training program is vital, as these young men and women will play an important role as future teachers and thereby, as role models helping to prevent childhood obesity and guiding children throughout their education towards a healthy life style.

RESUMEN: Este estudio presenta una comparación transversal de la característica morfológica de estudiantes de lengua húngara pertenecientes a la Facultad de Formación de Docentes en Subotica, Serbia, en función de su estado nutricional estimado con el estado de alimentación. La muestra estuvo compuesta por 146 adultos jóvenes, cursando los cuatro años en la Facultad, con edades comprendidas entre los 19 y los 23 años. Se realizaron las siguientes mediciones antropométricas: peso corporal, altura, grosor de la piel (pliegues cutáneos medidos en la escápula, tríceps, bíceps, ilion, cadera, muslo y abdomen) y circunferencias (cintura, cadera). Los autores calcularon y analizaron los datos, incluidos: el IMC (índice de masa corporal) con la fórmula, el grosor del pliegue cutáneo y con la ecuación del pliegue cutáneo de 4 sitios y la relación cintura-cadera. La estadística descriptiva se usó para describir las características morfológicas. Se realizaron análisis independientes de T-test y ANOVA para comparar los estudiantes de acuerdo al sexo y la edad. Los principales resultados del presente estudio son: (1) no se encontraron diferencias significativas entre los grupos de edad en el caso de la altura o el peso corporal, para los hombres jóvenes o las mujeres jóvenes; (2) se pudieron observar diferencias entre la altura y el peso en el caso del hombre y la mujer como características para este grupo etario; (3) las estudiantes, tienen con mayor frecuencia sobrepeso u obesidad, en relación a los estudiantes varones; (4) la mayoría de los estudiantes participantes pueden clasificarse en la categoría de un estado nutricional normal, lo cual es válido tanto para los hombres como para las mujeres jóvenes del estudio; (5) los porcentajes de grasa calculados basados en los valores del pliegue de la piel muestran que, en términos de peso, la mayoría de las alumnas se clasifican en la categoría "aceptable", mientras que la mayoría de los hombres jóvenes se clasifican como "delgados"; (6) en el caso del sobrepeso abdominal, el 20,0 % de los hombres y el 19,48 % de las mujeres pertenecen a la categoría 'obeso'. Esta información de los alumnos de la Facultad es vital, ya que estos jóvenes desempeñarán un papel importante como futuros docentes y como un modelo de conducta, ayudando de esta forma, a evitar la obesidad infantil y guiando a los niños a lo largo de su educación hacia un estilo de vida saludable.

Shared developmental roles and transcriptional control of autophagy and apoptosis in Caenorhabditis elegans.

Autophagy is a lysosome-mediated self-degradation process of eukaryotic cells that, depending on the cellular milieu, can either promote survival or act as an alternative mechanism of programmed cell death (PCD) in terminally differentiated cells. Despite the important developmental and medical implications of autophagy and the main form of PCD, apoptosis, orchestration of their regulation remains poorly understood. Here, we show in the nematode Caenorhabditis elegans, that various genetic and pharmacological interventions causing embryonic lethality trigger a massive cell death response that has both autophagic and apoptotic features. The two degradation processes are also redundantly required for normal development and viability in this organism. Furthermore, the CES-2-like basic region leucine-zipper (bZip) transcription factor ATF-2, an upstream modulator of the core apoptotic cell death pathway, is able to directly regulate the expression of at least two key autophagy-related genes, bec-1/ATG6 and lgg-1/ATG8. Thus, the two cell death mechanisms share a common method of transcriptional regulation. Together, these results imply that under certain physiological and pathological conditions, autophagy and apoptosis are co-regulated to ensure the proper morphogenesis and survival of the developing organism. The identification of apoptosis and autophagy as compensatory cellular pathways in C. elegans might help us to understand how dysregulated PCD in humans can lead to diverse pathologies, including cancer, neurodegeneration and diabetes.

Autophagy and apoptosis are redundantly required for C. elegans embryogenesis.

Apoptosis, the main form of regulated (or programmed) cell death, allows the organism to tightly control cell numbers and tissue size, and to protect itself from potentially damaging cells. This type of cellular self-killing has long been assumed to be essential for early development. In the nematode Caenorhabditis elegans, however, the core apoptotic cell death pathway appears to be dispensable for embryogenesis when most developmental cell deaths take place: mutant nematodes defective for apoptosis develop into adulthood, with superficially normal morphology and behavior. Accumulating evidence indicates a similar situation in mammalian systems as well. For example, apoptosis-deficient mice can grow as healthy, fertile adults. These observations raise the possibility that alternative cell death mechanisms may compensate for the lack of apoptotic machinery in developing embryos. Interestingly, C. elegans embryogenesis can also occur without autophagy, an alternative form of cellular self-destruction (also called autophagic cell death). In an upcoming paper we report that simultaneous inactivation of the autophagic and apoptotic gene cascades in C. elegans arrests development at early stages, and the affected embryos exhibit severe morphological defects. Double-mutant nematode embryos deficient in both autophagy and apoptosis are unable to undergo body elongation or to arrange several tissues correctly. This novel function of autophagy genes in morphogenesis indicates a more fundamental role for cellular self-digestion in tissue patterning than previously thought.

TRA-1/GLI controls the expression of the Hox gene lin-39 during C. elegans vulval development.

The vulva of the Caenorhabditis elegans hermaphrodite develops from a subset of six vulval precursor cells (VPCs) by the combined effect of the Ras, Wingless and Notch signaling cascades, and of three redundant synMuv (synthetic Multivulva) pathways grouped into classes A, B and C. Here we show that signaling via the GLI- (Glioma-associated protein) like transcription factor TRA-1, which is the terminal regulator of the C. elegans sex determination cascade, is a newly discovered pathway specifying vulval cell fates. We found that TRA-1 accumulates in, and regulates the fusion process of, cells (including the VPCs and hypodermal cells) involved in vulval patterning. TRA-1 also influenced the expression of the Hox gene lin-39, a central regulator of vulval development. Furthermore, inactivation of tra-1, which transforms animals with hermaphrodite-specific karyotype into males, promoted vulval induction in synMuv A, but not in synMuv B, mutant background. This implies that TRA-1 interacts with the class B synMuv genes, many of which are involved in chromatin-mediated transcriptional repression of cell proliferation. These results may help to understand how compromised GLI activity in humans leads to cancer. Together, we suggest that the GLI protein family involved in several key developmental processes in both invertebrates and vertebrates regulates somatic cell fates through influencing, at least in part, the expression of specific Hox genes.

Longevity pathways converge on autophagy genes to regulate life span in Caenorhabditis elegans.

Aging is a multifactorial process with many mechanisms contributing to the decline. Mutations decreasing insulin/IGF-1 (insulin-like growth factor-1) or TOR (target of rapamycin) kinase-mediated signaling, mitochondrial activity and food intake each extend life span in divergent animal phyla. Understanding how these genetically distinct mechanisms interact to control longevity is a fundamental and fascinating problem in biology. Here we show that mutational inactivation of autophagy genes, which are involved in the degradation of aberrant, damaged cytoplasmic constituents accumulating in all aging cells, accelerates the rate at which the tissues age in the nematode Caenorhabditis elegans. According to our results Drosophila flies deficient in autophagy are also short-lived. We further demonstrate that reduced activity of autophagy genes suppresses life span extension in mutant nematodes with inherent dietary restriction, aberrant insulin/IGF-1 or TOR signaling, and lowered mitochondrial respiration. These findings suggest that the autophagy gene cascade functions downstream of and is inhibited by different longevity pathways in C. elegans, therefore, their effects converge on autophagy genes to slow down aging and lengthen life span. Thus, autophagy may act as a central regulatory mechanism of animal aging.