ABSTRACT
Our objective in this study was to develop and assess the diagnostic value of a coagglutination test with making use of peptidoglycane of Staphylococcus aureus in the identification of diseases of staphylococcal etiology. A total of 166 patients with diseases of staphylococcal etiology were examined. A test was elaborated of coagglutination with making use of peptidoglycane of Staphylococcus aureus for a differential identification of antibodies to peptidoglycan in healthy persons and patients with staphylococcosis.
Subject(s)
Agglutination Tests/methods , Focal Infection/diagnosis , Maxillary Sinusitis/diagnosis , Otitis Media, Suppurative/diagnosis , Peptidoglycan , Staphylococcal Infections/diagnosis , Staphylococcus aureus , Tonsillitis/diagnosis , Adolescent , Agglutination Tests/statistics & numerical data , Antibodies, Bacterial/blood , Child , Child, Preschool , Chronic Disease , Humans , Infant , Peptidoglycan/immunology , Recurrence , Staphylococcus aureus/immunologyABSTRACT
Activity of transfer-factor (TF) delayed type hypersensitivity in allogenic and xenogeneic have studied. Guinea pigs was been sensibilized Soluble antigens and whole cells Candida albicans for production TF. Both TF expressed non specific and specific activity in the guinea pig and mouse leucocytes inhibition migration in vitro and delayed type hypersensitivity in mica. Activity of TF to cellular antigen was lower then TF to soluble antigen in xenogeneic systems. However TF activity was some low for whole cells in xenogeneic systems.
Subject(s)
Adjuvants, Immunologic/pharmacology , Antigens, Fungal/immunology , Candida albicans/immunology , Transfer Factor/immunology , Adjuvants, Immunologic/analysis , Animals , Cell Migration Inhibition , Dose-Response Relationship, Immunologic , Guinea Pigs , Hypersensitivity, Delayed/immunology , Immunity, Cellular , Immunization/methods , Mice , Transfer Factor/analysisABSTRACT
Frozen spleen of mice immunized with vaccine from S. sonnei has been homogenized in the isotonic saline solution and centrifuged. That supernatant has been shown to inhibit antibody immune response to thymus-dependent antigens (sheep and rabbit erythrocytes) and hypersensitivity of the delayed type to splenocytes of guinea pig. The supernatant did not inhibit the antibody formation to thymus-independent antigen (bacterial lipopolysaccharide). Immunosuppressive activity of the supernatant disappeared after treatment with anti-immunoglobulin (but not anti-O-antigen) immunosorbent. The spleen of immunized mice is concluded to contain a factor which inhibits T-cell link of the immune system and is of the immunoglobulin nature.
